Status of the ANAIS Dark Matter Project at the Canfranc Underground Laboratory

The ANAIS experiment aims at the confirmation of the DAMA/LIBRA signal. A detailed analysis of two NaI(Tl) crystals of 12.5 kg each grown by Alpha Spectra will be shown: effective threshold at 1 keVee is at reach thanks to outstanding light collection and robust PMT noise filtering protocols and the measured background is well understood down to 3 keVee, having quantified K, U and Th content and cosmogenic activation in the crystals. A new detector was installed in Canfranc in March 2015 together with the two previous modules and preliminary characterization results will be presented. Finally, the status and expected sensitivity of the full experiment with 112 kg will be reviewed.Comment: Contributed to the 11th Patras Workshop on Axions, WIMPs and WISPs, Zaragoza, June 22 to 26, 201

Background model of NaI(Tl) detectors for the ANAIS Dark Matter Project

A thorough understanding of the background sources is mandatory in any experiment searching for rare events. The ANAIS (Annual Modulation with NaI(Tl) Scintillators) experiment aims at the confirmation of the DAMA/LIBRA signal at the Canfranc Underground Laboratory (LSC). Two NaI(Tl) crystals of 12.5 kg each produced by Alpha Spectra have been taking data since December 2012. The complete background model of these detectors and more precisely in the region of interest will be described. Preliminary background analysis of a new 12.5 kg crystal received at Canfranc in March 2015 will be presented too. Finally, the power of anticoincidence rejection in the region of interest has been analyzed in a 4x 5 12.5 kg detector matrix.Comment: Contributed to the 11th Patras Workshop on Axions, WIMPs and WISPs, Zaragoza, June 22 to 26, 201

Analysis of backgrounds for the ANAIS-112 dark matter experiment

The ANAIS (Annual modulation with NaI(Tl) Scintillators) experiment aims at the confirmation or refutation of theDAMA/LIBRA positive annual modulation signal in the low energy detection rate, using the same target and technique, at the Canfranc Underground Laboratory (LSC) in Spain. ANAIS-112, consisting of nine 12.5 kg NaI(Tl) modules produced by Alpha Spectra Inc. in a 3x3matrix configuration, is taking data smoothly in "dark matter search" mode since August, 2017, after a commissioning phase and operation of the first detectors during the last years in various setups. A large effort has been carried out withinANAIS to characterize the background of sodium iodide detectors, before unblinding the data and performing the first annual modulation analysis. Here, the background models developed for all the nine ANAIS-112 detectors are presented. Measured spectra from threshold to high energy in different conditions are well described by the models based on quantified activities independently estimated following several approaches. In the region from 1 to 6 keVee the measured, efficiency corrected background level is 3.58+-0.02 keV-1 kg-1 day-1; NaI crystal bulk contamination is the dominant background source being 210Pb, 40K, 22Na and 3H contributions the most relevant ones. This background level, added to the achieved 1 keVee analysis threshold (thanks to the outstanding light collection and robust filtering procedures developed), allow ANAIS-112 to be sensitive to the modulation amplitude measured by DAMA/LIBRA, and able to explore at three sigma level in 5 years the WIMP parameter region singled out by this experiment.Comment: Final version for publicatio

Production and relevance of cosmogenic radionuclides in NaI(Tl) crystals

The cosmogenic production of long-lived radioactive isotopes in materials is an hazard for experiments demanding ultra-low background conditions. Although NaI(Tl) scintillators have been used in this context for a long time, very few activation data were available. We present results from two 12.5 kg NaI(Tl) detectors, developed within the ANAIS project and installed at the Canfranc Underground Laboratory. The prompt data taking starting made possible a reliable quantification of production of some I, Te and Na isotopes with half-lives larger than ten days. Initial activities underground were measured and then production rates at sea level were estimated following the history of detectors; a comparison of these rates with calculations using typical cosmic neutron flux at sea level and a selected description of excitation functions was also carried out. After including the contribution from the identified cosmogenic products in the detector background model, we found that the presence of 3H in the crystal bulk would help to fit much better our background model and experimental data. We have analyzed the cosmogenic production of 3H in NaI, and although precise quantification has not been attempted, we can conclude that it could imply a very relevant contribution to the total background below 15 keV in NaI detectors.Comment: Proceedings of the Low Radioactivity Techniques 2015 workshop, March 2015, Seattle (US

Study of scintillation in natural and synthetic quartz and methacrylate

Samples from different materials typically used as optical windows or light guides in scintillation detectors were studied in a very low background environment, at the Canfranc Underground Laboratory, searching for scintillation. A positive result can be confirmed for natural quartz: two distinct scintillation components have been identified, not being excited by an external gamma source. Although similar effect has not been observed neither for synthetic quartz nor for methacrylate, a fast light emission excited by intense gamma flux is evidenced for all the samples in our measurements. These results could affect the use of these materials in low energy applications of scintillation detectors requiring low radioactive background conditions, as they entail a source of background.Comment: Accepted for publication in Optical Material

Functional Effect of the p22phox -930A/G Polymorphism on p22phox Expression and NADPH Oxidase Activity in Hypertension

Oxidative stress induced by superoxide is implicated in hypertension. NADPH oxidase is the main source of superoxide in phagocytic and vascular cells, and the p22phox subunit is involved in NADPH oxidase activation. Recently we reported an association of 930A/G polymorphism in the human p22phox gene promoter with hypertension. This study was designed to investigate the functional role of this polymorphism in hypertension. We thus investigated the relationships between the 930A/G polymorphism and p22phox expression and NADPH oxidase–mediated superoxide production in phagocytic cells from 70 patients with essential hypertension and 70 normotensive controls. Genotyping of the polymorphism was performed by restriction fragment length polymorphism. NADPH oxidase activity was determined by chemiluminescence assays, and p22phox mRNA and protein expression was measured by Northern and Western blotting, respectively. Compared with hypertensive subjects with the AA/AG genotype, hypertensive subjects with the GG genotype exhibited increased (P 0.05) phagocytic p22phox mRNA (1.26 0.06 arbitrary unit [AU] versus 0.99 0.03 AU) and protein levels (0.58 0.05 AU versus 0.34 0.04 AU) and enhanced NADPH oxidase activity (1998 181 counts/s versus 1322 112 counts/s). No differences in these parameters were observed among genotypes in normotensive cells. Transfection experiments on vascular smooth muscle cells showed that the A-to-G substitution of this polymorphism produced an increased reporter gene expression in hypertensive cells. Nitric oxide production, as assessed by measurement of serum nitric oxide metabolites, was lower in GG hypertensive subjects than in AA/AG hypertensive subjects. In conclusion, these results suggest that hypertensive subjects carrying the GG genotype of the p22phox 930A/G polymorphism are highly exposed to NADPH oxidase-mediated oxidative stress

Association of increased phagocytic NADPH oxidasedependent superoxide production with diminished nitric oxide generation in essential hypertension

Objective: Oxidative stress has been implicated in the pathogenesis of hypertension and its complications through alterations in nitric oxide (NO) metabolism. This study was designed to investigate whether a relationship exists between phagocytic nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-dependent superoxide anion (•O2-) production and NO generation in patients with essential hypertension. Methods: Superoxide production was assayed by chemiluminescence under baseline and stimulated conditions on mononuclear cells obtained from hypertensives (n = 51) and normotensives (n = 43). NO production was evaluated by determining serum NO metabolites, nitrate plus nitrite (NOx). Results: Although there were no differences in baseline •O2- production between normotensives and hypertensives, the •O2- production in phorbol myristate acetate (PMA)-stimulated mononuclear cells was increased (P < 0.05) in hypertensives compared with normotensives. The PMA-induced •O2- production was completely abolished by apocynin, a specific inhibitor of NADPH oxidase. Moreover, stimulation of •O2- production by angiotensin II and endothelin-1 was higher (P < 0.05) in cells from hypertensives than in cells from normotensives. In addition, diminished (P < 0.001) serum NOx was detected in hypertensives compared with normotensives. Interestingly, an inverse correlation (r = 0.493, P < 0.01) was found between •O2- production and NOx in hypertensives. Conclusions: Generation of •O2- mainly dependent on NADPH oxidase is abnormally enhanced in stimulated mononuclear cells from hypertensives. It is suggested that this alteration could be involved in the diminished NO production observed in these patients