Особенности представления малой родины в материалах духовной периодики Курской губернии конца XIX — начала XX века

Creative works of amateur authors are significant in the reflection of ethnic culture in the provincial periodicals. In the publications of “Kurskie eparhialinie vedomosti” (“Kursk diocesan Gazette”) the fact of existence and dynamic development of the clergy subculture in the Kursk province was fixed. The study of peculiarities concerning the concept of “a Small Motherland” in the poetic experience of provincial authors will allow to reconstruct the important parts of the cultural space in pre-1917 Russian province and also to promote the deep understanding of provinces’ role in literary process at the end of the XIX — the beginning of the XX century.Творчество непрофессиональных авторов занимает существенное место как отражение культуры этноса в провинциальной периодике. «Курские епархиальные ведомости» в своих публикациях зафиксировали факт существования и динамичного развития субкультуры духовенства Курской губернии. Изучение особенностей концепта «малой родины» в поэтических опытах местных авторов позволит реконструировать значимые области культурного пространства Российской провинции дооктябрьской поры, а также способствовать глубокому пониманию роли провинции в литературном процессе XIX — начала XX века

Love as a Fictitious Commodity: Gift-for-Sex Barters as Contractual Carriers of Intimacy

Abstract Gift-for-sex (GFS) barters are a niche practice potentially representing the commodification of everyday dating practices. We inquire how GFS exchanges are practiced and understood in contemporary Russia. Second, we situate these in relation to contemporary economic culture. Our project provides answers in two steps based on online content. First, we identify GFS exchange practices within a major dating website. Next, we take the signals exchanged in those dating profiles and display their intersubjective meanings in Russia based on blogs and discussion fora. Our analysis focuses on gender roles and inter-gender conflicts, the use of economic jargon, the link between luxury consumption and sexuality, and understandings of gift-giving and generosity, in order to show how GFS barters, despite being contractual, carry emotional and romantic content. As such, love is under a constant conversion process, through the medium of the contractual gift, into the fictitious commodity form

Mechanical overstimulation causes acute injury and synapse loss followed by fast recovery in lateral-line neuromasts of larval zebrafish

Excess noise damages sensory hair cells, resulting in loss of synaptic connections with auditory nerves and, in some cases, hair-cell death. The cellular mechanisms underlying mechanically induced hair-cell damage and subsequent repair are not completely understood. Hair cells in neuromasts of larval zebrafish are structurally and functionally comparable to mammalian hair cells but undergo robust regeneration following ototoxic damage. We therefore developed a model for mechanically induced hair-cell damage in this highly tractable system. Free swimming larvae exposed to strong water wave stimulus for 2 hr displayed mechanical injury to neuromasts, including afferent neurite retraction, damaged hair bundles, and reduced mechanotransduction. Synapse loss was observed in apparently intact exposed neuromasts, and this loss was exacerbated by inhibiting glutamate uptake. Mechanical damage also elicited an inflammatory response and macrophage recruitment. Remarkably, neuromast hair-cell morphology and mechanotransduction recovered within hours following exposure, suggesting severely damaged neuromasts undergo repair. Our results indicate functional changes and synapse loss in mechanically damaged lateral-line neuromasts that share key features of damage observed in noise-exposed mammalian ear. Yet, unlike the mammalian ear, mechanical damage to neuromasts is rapidly reversible

Na,K-ATPase Acts as a Beta-Amyloid Receptor Triggering Src Kinase Activation

Beta-amyloid (Aβ) has a dual role, both as an important factor in the pathology of Alzheimer’s disease and as a regulator in brain physiology. The inhibitory effect of Aβ42 oligomers on Na,K-ATPase contributes to neuronal dysfunction in Alzheimer’s disease. Still, the physiological role of the monomeric form of Aβ42 interaction with Na,K-ATPase remains unclear. We report that Na,K-ATPase serves as a receptor for Aβ42 monomer, triggering Src kinase activation. The co-localization of Aβ42 with α1- and β1-subunits of Na,K-ATPase, and Na,K-ATPase with Src kinase in SH-SY5Y neuroblastoma cells, was observed. Treatment of cells with 100 nM Aβ42 causes Src kinase activation, but does not alter Na,K-ATPase transport activity. The interaction of Aβ42 with α1β1 Na,K-ATPase isozyme leads to activation of Src kinase associated with the enzyme. Notably, prevention of Na,K-ATPase:Src kinase interaction by a specific inhibitor pNaKtide disrupts the Aβ-induced Src kinase activation. Stimulatory effect of Aβ42 on Src kinase was lost under hypoxic conditions, which was similar to the effect of specific Na,K-ATPase ligands, the cardiotonic steroids. Our findings identify Na,K-ATPase as a Aβ42 receptor, thus opening a prospect on exploring the physiological and pathological Src kinase activation caused by Aβ42 in the nervous system

Solid state synthesis of carbon-encapsulated iron carbide nanoparticles and their interaction with living cells †

Superparamagnetic carbon-encapsulated iron carbide nanoparticles (NPs), Fe 7 C 3 @C, with unique properties, were produced from pure ferrocene by high pressure-high temperature synthesis. These NPs combine the merits of nanodiamonds and SPIONs but lack their shortcomings which limit their use for biomedical applications. Investigation of these NPs by X-ray diffraction, electron microscopy techniques, X-ray spectroscopic and magnetic measurement methods has demonstrated that this method of synthesis yields NPs with perfectly controllable physical properties. Using magnetic and subsequent fractional separation of magnetic NPs from residual carbon, the aqueous suspensions of Fe 7 C 3 @C NPs with an average particle size of $25 nm were prepared. The suspensions were used for in vitro studies of the interaction of Fe 7 C 3 @C NPs with cultured mammalian cells. The dynamics of interaction of the living cells with Fe 7 C 3 @C was studied by optical microscopy using time-lapse video recording and also by transmission electron microscopy. Using novel highly sensitive cytotoxicity tests based on the cell proliferation assay and long-term live cell observations it was shown that the internalization of Fe 7 C 3 @C NPs has no cytotoxic effect on cultured cells and does not interfere with the process of their mitotic division, a fundamental property that ensures the existence of living organisms. The influence of NPs on the proliferative activity of cultured cells was not detected as well. These results indicate that the carbon capsules of Fe 7 C 3 @C NPs are air-tight which could offer great opportunities for future use of these superparamagnetic NPs in biology and medicine

PUBLICISTIC METHODS PROVINCIAL SPIRITUAL PERIODICALS (ILLUSTRATED EDITION "KURSK DIOCESAN GAZETTE," THE 80-S OF THE XIX CENTURY)

Материал статьи основан на анализе специфики материалов провинци- альной духовной периодики 80-х годов XIX века, особое внимание уделено публици- стическим приёмам, демонстрирующим самобытность духовной журналистики дооктябрьского периода. Изучение региональной духовной периодики будет спо- собствовать построению целостной системы представлений о духовной жизни народа и всестороннему осмыслению такого явления культурной жизни России, как духовная литературная традиция.The material is based on an analysis of specific materials provincial spiritual periodicals 80-s of the XIX century, it focuses on a publicist techniques, demonstrating the originality of the spiritual journalism pre-October period. The study of Regional Spiritual journalism will contribute to building a coherent system of ideas about the spiritual life of Russian province and comprehensive understanding the phenomenon of cultural life in Russia as spiritual literary tradition

Foreign Teaching Experience Addressing Literacy Deficiency in the ICT Era

The last decade witnessed a decline in the level of reading literacy among Russian students according to the result of the PISA International study. World social changes of 2021 stipulate new approaches to functional literacy, require the modernization of both pedagogical activities and the development of a long-term educational strategy. International pedagogical experience demonstrates a significant role of non-profit literacy organizations that provide online resources for students and schoolteachers. The purpose of the study is to reveal specific features and functions of literacy organizations in the UK, the USA, and Australia. In this paper, the authors explore the possibility of subsequent implementation of relevant foreign experience in Russia to increase the level of students’ functional literacy. The study is based on the data obtained in foreign methodological and pedagogical sources. The leading approaches include systematic and comparative analysis, methods of definition and classification. The results of this study can be further used for the development of a non-profit literacy organization with the purpose of functional literacy improvement of school students in Russia. The materials of the article will be useful for researchers of foreign pedagogics, educators and schoolteachers, extra-curriculum courses developers

Love as a Fictitious Commodity: Gift-for-Sex Barters as Contractual Carriers of Intimacy

Gift-for-sex (GFS) barters are a niche practice potentially representing the commodification of everyday dating practices. We inquire how GFS exchanges are practiced and understood in contemporary Russia. Second, we situate these in relation to contemporary economic culture. Our project provides answers in two steps based on online content. First, we identify GFS exchange practices within a major dating website. Next, we take the signals exchanged in those dating profiles and display their intersubjective meanings in Russia based on blogs and discussion fora. Our analysis focuses on gender roles and inter-gender conflicts, the use of economic jargon, the link between luxury consumption and sexuality, and under- standings of gift-giving and generosity, in order to show how GFS barters, despite being contractual, carry emotional and romantic content. As such, love is under a constant conversion process, through the medium of the contractual gift, into the fictitious commodity form

Osteoclasts Differentiation from Murine RAW 264.7 Cells Stimulated by RANKL: Timing and Behavior

The development of multi-nucleated cells is critical for osteoclasts (OCs) maturation and function. Our objective was to extend knowledge on osteoclastogenesis, focusing on pre-OC fusion timing and behavior. RAW 264.7 cells, which is a murine monocyte-macrophage cell line, provide a valuable and widely used tool for in vitro studies on osteoclastogenesis mechanisms. Cells were treated with the receptor activator of nuclear factor κ-B ligand (RANKL) for 1–4 days and effects on cell morphology, cytoskeletal organization, protein distribution, and OC-specific gene expression examined by TEM, immunofluorescence, and qPCR. Multinucleated cells began to appear at two days of Receptor Activator of Nuclear factor κ-B Ligand (RANKL) stimulation, increasing in number and size in the following days, associated with morphological and cytoskeletal organization changes. Interesting cellular extensions were observed in three days within cells labeled with wheat germ agglutinin (WGA)-Fluorescein isothiocyanate (FITC). The membrane, cytoplasmic, or nuclear distribution of RANK, TRAF6, p-p38, pERK1/2, and NFATc1, respectively, was related to OCs maturation timing. The gene expression for transcription factors regulating osteoclastogenesis (NFATc1, c-fos, RelA, MITF), molecules involved in RANKL-signaling transduction (TRAF6), cytoskeleton regulation (RhoA), fusion (DC-STAMP), migration (MMP9), and OC-specific enzymes (TRAP, CtsK), showed different trends related to OC differentiation timing. Our findings provide an integrated view on the morphological and molecular changes occurring during RANKL stimulation of RAW 264.7 cells, which are important to better understand the OCs’ maturation processes

Osteoclasts Differentiation from Murine RAW 264.7 Cells Stimulated by RANKL: Timing and Behavior

The development of multi-nucleated cells is critical for osteoclasts (OCs) maturation and function. Our objective was to extend knowledge on osteoclastogenesis, focusing on pre-OC fusion timing and behavior. RAW 264.7 cells, which is a murine monocyte-macrophage cell line, provide a valuable and widely used tool for in vitro studies on osteoclastogenesis mechanisms. Cells were treated with the receptor activator of nuclear factor κ-B ligand (RANKL) for 1–4 days and effects on cell morphology, cytoskeletal organization, protein distribution, and OC-specific gene expression examined by TEM, immunofluorescence, and qPCR. Multinucleated cells began to appear at two days of Receptor Activator of Nuclear factor κ-B Ligand (RANKL) stimulation, increasing in number and size in the following days, associated with morphological and cytoskeletal organization changes. Interesting cellular extensions were observed in three days within cells labeled with wheat germ agglutinin (WGA)-Fluorescein isothiocyanate (FITC). The membrane, cytoplasmic, or nuclear distribution of RANK, TRAF6, p-p38, pERK1/2, and NFATc1, respectively, was related to OCs maturation timing. The gene expression for transcription factors regulating osteoclastogenesis (NFATc1, c-fos, RelA, MITF), molecules involved in RANKL-signaling transduction (TRAF6), cytoskeleton regulation (RhoA), fusion (DC-STAMP), migration (MMP9), and OC-specific enzymes (TRAP, CtsK), showed different trends related to OC differentiation timing. Our findings provide an integrated view on the morphological and molecular changes occurring during RANKL stimulation of RAW 264.7 cells, which are important to better understand the OCs’ maturation processes