THE COMPARATIVE EVALUATION OF THE BIOTECHNOLOGICAL PROCESSES OF STERILIZATION AND PRESERVATION, TO BE USED IN THE PRODUCTION OF THE CHOLERA BIVALENT CHEMICAL TABLETTE VACCINE

The method of preservation, using the merthyolate of the high-immunogenous fractions, included in the composition of the cholera vaccine, has been offered. The antigenic composition of the preparation components, obtained by the improved technology, has been studied. The harmfulness of the given method of preservation of the microorganism has been shown. The biotechnological ways of the mass receiving the vaccine with use of the optimum exposition and minimum preserving dose of merthyolate have been developed. The experimentally-production series of the cholera chemical bivalent tabletted vaccine, obtained with use of the merthyolate as the preservant, was permitted by the GISKAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

Nutritional and energy value of muscle tissue of Limousine bulls of different genotypes

The article presents the results of a comparative study of the nutritional and energy value of beef obtained from bull calves of different genotypes. The aim of the study was to evaluate the nutritional and energy value of beef obtained from Limousin bulls of different genotypes. The research objects were half carcasses obtained as a result of control slaughter of Limousin breed bull calves, descendants of mothers of different origin, bred in the Kuyurgazinsky district of the Republic of Bashkortostan in the "Meat Union of Bashkir producers" Ltd. The bull calves were raised according to the cow-calf system, weaning from mothers was carried out at the age of 8 months before stabling, then growing up, the first and second periods of fattening were carried out. Control slaughter of bull calves was carried out when they reached the required meat condition at the age of 18 months. In the course of the studies of dry matter, protein, extracted fat and energy value of carcass muscle tissue, a significant increase in the dry matter, protein, extracted fat, and energy content was observed (p≤0.05) 8.8kg higher performance of bull calves with mothers of French origin compared to carcasses of bull calves from mothers of Australian origin (11.8%), at 5.3kg (12.8%), at 3.1 kg (10.1%) and at 248.6 MJ (12.8%) accordingly. Based on the data obtained, it can be assumed that the breeding of animals for a long period of time under conditions of different housing and feeding technologies influences the chemical composition characteristics and the energy value of the meat of the representatives of the Limousin cattle breed studied

Visual outcomes of small-incision lenticule extraction (SMILE) in thin corneas

We aimed to find out whether thin (≤500 μm) or normal (>500 μm, control) corneal thickness would impact efficacy and safety outcomes of small-incision lenticule extraction (SMILE). We retrospectively analyzed medical records of adult patients who had undergone SMILE. A total of 57 eyes were included in the "thin corneas" group and 180 eyes in the "control" group. At one month after surgery, rates of patients with uncorrected distance visual activity (UDVA) ≥ 0.8 were significantly higher in patients from the control group compared to the "thin corneas" group (87 vs. 71%, respectively p < 0.01), though rates were comparable at 3 months (87 vs. 76%, respectively, p > 0.05). SMILE had comparable safety in patients with thin and normal corneas. Procedure result predictability was comparable between groups. Regression analysis demonstrated that cap thickness impacted posterior corneal biomechanics, and the volume of removed tissue had a higher influence in patients with thin corneas. Moreover, an increase in cap thickness was associated with better final BCVA. Further study is needed for the evaluation of the impact of thin corneas on SMILE outcomes and planning. Our study also indicates that patients with thin corneas might require a different approach to nomogram calculation

Thrombin generation test for evaluation of antiplatelet treatment in patients with coronary artery disease after percutaneous coronary intervention

To study the possibility of using thrombin generation tests in platelet-rich and platelet-poor plasma for evaluation of dual antiplatelet therapy efficacy in patients with coronary artery disease (CAD), following percutaneous coronary intervention. Venous blood was analyzed from CAD patients aged 53–75 years who had undergone percutaneous coronary intervention with stenting within one year and had been receiving standard doses of clopidogrel and aspirin (75 and 75–100 mg per day, respectively). The control group comprised age- and sex-matched subjects without clinical signs of CAD who were not receiving these drugs. Thrombin generation tests were performed in platelet-rich and platelet-poor plasma. Intravascular platelet activation, induced platelet aggregation, and routine coagulation were evaluated. Antiplatelet treatment did not influence results of routine coagulation tests or intravascular platelet activation. The dual antiplatelet therapy affects collagen-induced platelet aggregation (44 ± 2.5 vs. 7.9 ± 2.6%, р = 10−7) and leads to decreases in endogenous thrombin potential (1900 ± 85 vs. 1740 ± 95 nM∙min, p = 0.0045), maximum thrombin concentration (134 ± 9.5 vs. 106 ± 6.5 nM, p = 4∙10−6), and increases in time to peak thrombin (27 ± 1.5 vs. 31 ± 2 min, p = 0.0012). Decreases in thrombin generation rate showed the highest statistical significance (13 ± 2 vs. 7.9 ± 0.8 nM/min, p = 10−8). Antiplatelet treatment did not alter thrombogram parameters for platelet-poor plasma