2 research outputs found
Effect of platelet-rich fibrin matrix in complex with artificial material Nubiplant on expression of chondrogenic marker genes and morphogenesis of the nucleus pulposus cells of intervertebral discs in rats
The purpose was to study the barrier and biological properties of platelet-rich fibrin matrix (PRFM), an artificial biopolymer Nubiplant, and a mixture of PRFM / Nubiplant by assessing the viability and morphological characteristics of nucleus pulposus (NP) cells in rats, as well as the expression level of chondrogenic marker genes during cell cultivation in the presence of these matrices.Materials and methods. PRFM was obtained from platelet-rich plasma using a SiO2 coagulation activator. A suspension of nucleus pulposus cells was obtained from the caudal spine of rats. Cultivation was carried out in the presence of one of three matrices β PRFM, Nubiplant, or their mixture for 3, 7, and 14 days under standard culture conditions in an EC-160 incubator (NΓΌve, Turkey). Observation of the living culture was carried out in the area bordering with the matrix within one field of view using an inverted microscope (Nicon TS100, Japan). The expression of chondrogenic marker genes in the cell culture of the NP was determined by the method of PCR with reverse transcription.Results. The study of the viability and morphological characteristics of NP cells during their cultivation for 3, 7, and 14 days in the presence of PRFM, PRFM / Nubiplant, or Nubiplant showed a decrease in the content of living cells in control samples; in cultures with PRFM and PRFM / Nubiplant, the number of living cells significantly exceeded the control values, aggregation of cells was observed in the area bordering with the matrices from the side of the application. None of the experimental samples showed the outflow of cells to the opposite side of the matrix after 14 days of cultivation; thus, PRFM, Nubiplant, and their mixture can perform barrier functions to keep the cell population in a certain location. Expression of the COL II, ACAN, GPC3, ANXA3, PTN, MGP, and VIM genes by the NP cells during cultivation for 3 and 7 days in the presence of PRFM and PRFM / Nubiplant increased as compared to the control samples.Conclusions. The use of PRFM, Nubiplant, or a mixture of PRFM / Nubiplant during the cultivation of NP cells demonstrated the absence of cell outflow to the opposite side of the studied matrices during the study period (14 days). The use of PRFM, Nubiplant, or a mixture of PRFM / Nubiplant promoted the formation of cell colonies with chondrocyte-like morphology in the zone bordering with the matrices and maintained cell viability throughout the study period. PRFM and PRFM / Nubiplant contributed to the maintenance of the expression of chondrogenic genes in the NP cells in the zone bordering the matrices. The results obtained indicate the positive effect of the matrix based on platelet-rich fibrin on the NP cells and its barrier functions, which is promising for the use of PRMF for preventing the formation of cicatricial adhesion
ΠΠΏΠ»ΠΈΠ² Π·Π±Π°Π³Π°ΡΠ΅Π½ΠΎΠ³ΠΎ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΠΌΠΈ ΡΡΠ±ΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ°ΡΡΠΈΠΊΡΡ Π² ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡ Π·Ρ ΡΡΡΡΠ½ΠΈΠΌ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»ΠΎΠΌ Nubiplant Π½Π° Π΅ΠΊΡΠΏΡΠ΅ΡΡΡ Ρ ΠΎΠ½Π΄ΡΠΎΠ³Π΅Π½Π½ΠΈΡ ΠΌΠ°ΡΠΊΠ΅ΡΠ½ΠΈΡ Π³Π΅Π½ΡΠ² ΡΠ° ΠΌΠΎΡΡΠΎΠ³Π΅Π½Π΅Π· ΠΊΠ»ΡΡΠΈΠ½ ΠΏΡΠ»ΡΠΏΠΎΠ·Π½ΠΎΠ³ΠΎ ΡΠ΄ΡΠ° ΠΌΡΠΆΡ ΡΠ΅Π±ΡΠ΅Π²ΠΈΡ Π΄ΠΈΡΠΊΡΠ² ΡΡΡΡΠ²
The purpose was to study the barrier and biological properties of platelet-rich fibrin matrix (PRFM), an artificial biopolymer Nubiplant, and a mixture of PRFM / Nubiplant by assessing the viability and morphological characteristics of nucleus pulposus (NP) cells in rats, as well as the expression level of chondrogenic marker genes during cell cultivation in the presence of these matrices.Materials and methods. PRFM was obtained from platelet-rich plasma using a SiO2 coagulation activator. A suspension of nucleus pulposus cells was obtained from the caudal spine of rats. Cultivation was carried out in the presence of one of three matrices β PRFM, Nubiplant, or their mixture for 3, 7, and 14 days under standard culture conditions in an EC-160 incubator (NΓΌve, Turkey). Observation of the living culture was carried out in the area bordering with the matrix within one field of view using an inverted microscope (Nicon TS100, Japan). The expression of chondrogenic marker genes in the cell culture of the NP was determined by the method of PCR with reverse transcription.Results. The study of the viability and morphological characteristics of NP cells during their cultivation for 3, 7, and 14 days in the presence of PRFM, PRFM / Nubiplant, or Nubiplant showed a decrease in the content of living cells in control samples; in cultures with PRFM and PRFM / Nubiplant, the number of living cells significantly exceeded the control values, aggregation of cells was observed in the area bordering with the matrices from the side of the application. None of the experimental samples showed the outflow of cells to the opposite side of the matrix after 14 days of cultivation; thus, PRFM, Nubiplant, and their mixture can perform barrier functions to keep the cell population in a certain location. Expression of the COL II, ACAN, GPC3, ANXA3, PTN, MGP, and VIM genes by the NP cells during cultivation for 3 and 7 days in the presence of PRFM and PRFM / Nubiplant increased as compared to the control samples.Conclusions. The use of PRFM, Nubiplant, or a mixture of PRFM / Nubiplant during the cultivation of NP cells demonstrated the absence of cell outflow to the opposite side of the studied matrices during the study period (14 days). The use of PRFM, Nubiplant, or a mixture of PRFM / Nubiplant promoted the formation of cell colonies with chondrocyte-like morphology in the zone bordering with the matrices and maintained cell viability throughout the study period. PRFM and PRFM / Nubiplant contributed to the maintenance of the expression of chondrogenic genes in the NP cells in the zone bordering the matrices. The results obtained indicate the positive effect of the matrix based on platelet-rich fibrin on the NP cells and its barrier functions, which is promising for the use of PRMF for preventing the formation of cicatricial adhesion.Π¦Π΅Π»Ρ: ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ Π±Π°ΡΡΠ΅ΡΠ½ΡΠ΅ ΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΠΌΠΈ ΡΠΈΠ±ΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ°ΡΡΠΈΠΊΡΠ° (ΠΠ’Π€Π), ΠΈΡΠΊΡΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π±ΠΈΠΎΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ° Nubiplant ΠΈ ΡΠΌΠ΅ΡΠΈ ΠΠ’Π€Π ΠΈ Nubiplant ΠΏΡΡΠ΅ΠΌ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΈ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΡΠ»ΡΠΏΠΎΠ·Π½ΠΎΠ³ΠΎ ΡΠ΄ΡΠ° (ΠΠ―) ΠΌΠ΅ΠΆΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΡΡ
Π΄ΠΈΡΠΊΠΎΠ² ΠΊΡΡΡ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΠΎΠ²Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Ρ
ΠΎΠ½Π΄ΡΠΎΠ³Π΅Π½Π½ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠ½ΡΡ
Π³Π΅Π½ΠΎΠ² ΠΏΡΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΡΠΈΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΡΠΈ Π½Π°Π»ΠΈΡΠΈΠΈ ΡΠΊΠ°Π·Π°Π½Π½ΡΡ
ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠ².ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ’Π€Π ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΠΈΠ· ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½Π½ΠΎΠΉ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΠΌΠΈ ΠΏΠ»Π°Π·ΠΌΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°ΠΊΡΠΈΠ²Π°ΡΠΎΡΠ° ΡΠ²Π΅ΡΡΡΠ²Π°Π½ΠΈΡ SiO2, ΡΡΡΠΏΠ΅Π½Π·ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΠ― β ΠΈΠ· Ρ
Π²ΠΎΡΡΠΎΠ²ΠΎΠ³ΠΎ ΠΎΡΠ΄Π΅Π»Π° ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΠΈΠΊΠ° ΠΊΡΡΡ. ΠΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ ΠΏΡΠΈ Π½Π°Π»ΠΈΡΠΈΠΈ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΈΠ· ΡΡΠ΅Ρ
ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠ² β ΠΠ’Π€Π, Nubiplant ΠΈΠ»ΠΈ ΠΈΡ
ΡΠΌΠ΅ΡΠΈ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 3, 7 ΠΈ 14 ΡΡΡΠΎΠΊ ΠΏΡΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΡΡ
ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΡΡ
Π² ΠΈΠ½ΠΊΡΠ±Π°ΡΠΎΡΠ΅ ΠΠ‘-160 (NΓΌve, Π’ΡΡΡΠΈΡ). ΠΠ°Π±Π»ΡΠ΄Π΅Π½ΠΈΠ΅ Π·Π° ΠΆΠΈΠ²ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΠΎΠΉ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π² ΠΏΠΎΠ³ΡΠ°Π½ΠΈΡΠ½ΠΎΠΉ Ρ ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠΌ Π·ΠΎΠ½Π΅ Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ»Ρ Π·ΡΠ΅Π½ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΈΠ½Π²Π΅ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠ° (Nicon TS100, Π―ΠΏΠΎΠ½ΠΈΡ). ΠΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Ρ
ΠΎΠ½Π΄ΡΠΎΠ³Π΅Π½Π½ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠ½ΡΡ
Π³Π΅Π½ΠΎΠ² Π² ΠΊΡΠ»ΡΡΡΡΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΠ― ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ Ρ ΠΎΠ±ΡΠ°ΡΠ½ΠΎΠΉ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠ΅ΠΉ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΈ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΠ― ΠΏΡΠΈ ΠΈΡ
ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π»ΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΆΠΈΠ²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
. Π ΠΊΡΠ»ΡΡΡΡΠ°Ρ
Ρ ΠΠ’Π€Π ΠΈ ΡΠΌΠ΅ΡΡΡ ΠΠ’Π€Π ΠΈ Nubiplant ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΆΠΈΠ²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎ ΠΏΡΠ΅Π²ΡΡΠ°Π»ΠΎ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ. ΠΠ°Π±Π»ΡΠ΄Π°Π»ΠΈ Π°Π³ΡΠ΅Π³Π°ΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΠΏΠΎΠ³ΡΠ°Π½ΠΈΡΠ½ΠΎΠΉ Ρ ΠΌΠ°ΡΡΠΈΠΊΡΠ°ΠΌΠΈ Π·ΠΎΠ½Π΅ ΡΠΎ ΡΡΠΎΡΠΎΠ½Ρ Π½Π°Π½Π΅ΡΠ΅Π½ΠΈΡ. ΠΡΡΠ΅Π»Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π½Π° ΠΏΡΠΎΡΠΈΠ²ΠΎΠΏΠΎΠ»ΠΎΠΆΠ½ΡΡ ΡΡΠΎΡΠΎΠ½Ρ ΠΌΠ°ΡΡΠΈΠΊΡΠ° Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 14 ΡΡΡΠΎΠΊ Π½ΠΈ Π² ΠΎΠ΄Π½ΠΎΠΌ ΠΈΠ· ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Π½Π΅ ΠΎΡΠΌΠ΅ΡΠ΅Π½ΠΎ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΠΠ’Π€Π, Nubiplant ΠΈ ΠΈΡ
ΡΠΌΠ΅ΡΡ ΠΌΠΎΠ³ΡΡ Π²ΡΠΏΠΎΠ»Π½ΡΡΡ Π±Π°ΡΡΠ΅ΡΠ½ΡΠ΅ ΡΡΠ½ΠΊΡΠΈΠΈ ΠΏΠΎ ΡΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΈ Π² ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΠΎΠΌ ΡΡΠ°ΡΡΠΊΠ΅. ΠΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π³Π΅Π½ΠΎΠ² COL II, ACAN, GPC3, ANXA3, PTN, MGP ΠΈ VIM ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΠΠ― ΠΏΡΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 3 ΠΈ 7 ΡΡΡΠΎΠΊ ΠΏΡΠΈ Π½Π°Π»ΠΈΡΠΈΠΈ ΠΠ’Π€Π ΠΈ ΡΠΌΠ΅ΡΠΈ ΠΠ’Π€Π ΠΈ Nubiplant Π²ΠΎΠ·ΡΠ°ΡΡΠ°Π»Π° ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΠΌΠΈ ΠΎΠ±ΡΠ°Π·ΡΠ°ΠΌΠΈ.ΠΡΠ²ΠΎΠ΄Ρ. ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΠ’Π€Π, Nubiplant ΠΈΠ»ΠΈ ΡΠΌΠ΅ΡΠΈ ΠΠ’Π€Π ΠΈ Nubiplant ΠΏΡΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΠ― ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π»ΠΎ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ Π²ΡΡΠ΅Π»Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π½Π° ΠΏΡΠΎΡΠΈΠ²ΠΎΠΏΠΎΠ»ΠΎΠΆΠ½ΡΡ ΡΡΠΎΡΠΎΠ½Ρ ΡΠΏΠΎΠΌΡΠ½ΡΡΡΡ
ΠΌΠ°ΡΡΠΈΠΊΡΠΎΠ² Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ Π²ΡΠ΅Π³ΠΎ ΠΏΠ΅ΡΠΈΠΎΠ΄Π° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ (14 Π΄Π½Π΅ΠΉ). ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠ’Π€Π, Nubiplant ΠΈΠ»ΠΈ ΡΠΌΠ΅ΡΠΈ ΠΠ’Π€Π ΠΈ Nubiplant ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΠΎΠ²Π°Π»ΠΎ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠΉ ΠΊΠ»Π΅ΡΠΎΠΊ Ρ
ΠΎΠ½Π΄ΡΠΎΡΠΈΡΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠΉ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Π² ΠΏΠΎΠ³ΡΠ°Π½ΠΈΡΠ½ΠΎΠΉ Ρ ΠΌΠ°ΡΡΠΈΠΊΡΠ°ΠΌΠΈ Π·ΠΎΠ½Π΅ ΠΈ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΈΠ²Π°Π»ΠΎ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ Π²ΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠ³ΠΎ ΠΏΠ΅ΡΠΈΠΎΠ΄Π°. ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΠ’Π€Π ΠΈ ΠΠ’Π€Π ΠΈ Nubiplant ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ»ΠΎ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΈΠ²Π°ΡΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Ρ
ΠΎΠ½Π΄ΡΠΎΠ³Π΅Π½Π½ΡΡ
Π³Π΅Π½ΠΎΠ² ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΠΠ― Π² ΠΏΠΎΠ³ΡΠ°Π½ΠΈΡΠ½ΠΎΠΉ Ρ ΠΌΠ°ΡΡΠΈΠΊΡΠ°ΠΌΠΈ Π·ΠΎΠ½Π΅. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ Π΄Π΅ΠΉΡΡΠ²ΠΈΠΈ ΠΌΠ°ΡΡΠΈΠΊΡΠ° Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΠΌΠΈ ΡΠΈΠ±ΡΠΈΠ½Π° Π½Π° ΠΊΠ»Π΅ΡΠΊΠΈ ΠΠ― ΠΈ Π΅Π³ΠΎ Π±Π°ΡΡΠ΅ΡΠ½ΡΠ΅ ΡΡΠ½ΠΊΡΠΈΠΈ, ΡΡΠΎ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ Π΄Π»Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΠ’Π€Π Ρ ΡΠ΅Π»ΡΡ ΠΏΡΠ΅Π΄ΠΎΡΠ²ΡΠ°ΡΠ΅Π½ΠΈΡ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ±ΡΠΎΠ²ΠΎ-ΡΠΏΠ°Π΅ΡΠ½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ².ΠΠ΅ΡΠ°: Π΄ΠΎΡΠ»ΡΠ΄ΠΈΡΠΈ Π±Π°ΡβΡΡΠ½Ρ ΡΠ° Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½Ρ Π²Π»Π°ΡΡΠΈΠ²ΠΎΡΡΡ Π·Π±Π°Π³Π°ΡΠ΅Π½ΠΎΠ³ΠΎ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΠΌΠΈ ΡΡΠ±ΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ°ΡΡΠΈΠΊΡΡ (ΠΠ’Π€Π), ΡΡΡΡΠ½ΠΎΠ³ΠΎ Π±ΡΠΎΠΏΠΎΠ»ΡΠΌΠ΅ΡΡ Nubiplant ΡΠ° ΡΡ
ΡΡΠΌΡΡΡ ΡΠ»ΡΡ
ΠΎΠΌ ΠΎΡΡΠ½ΠΊΠΈ ΠΆΠΈΡΡΡΠ·Π΄Π°ΡΠ½ΠΎΡΡΡ ΡΠ° ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΈΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΠΊΠ»ΡΡΠΈΠ½ ΠΏΡΠ»ΡΠΏΠΎΠ·Π½ΠΎΠ³ΠΎ ΡΠ΄ΡΠ° (ΠΠ―) ΠΌΡΠΆΡ
ΡΠ΅Π±ΡΠ΅Π²ΠΈΡ
Π΄ΠΈΡΠΊΡΠ² ΡΡΡΡΠ², Π° ΡΠ°ΠΊΠΎΠΆ ΡΡΠ²Π½Ρ Π΅ΠΊΡΠΏΡΠ΅ΡΡΡ Ρ
ΠΎΠ½Π΄ΡΠΎΠ³Π΅Π½Π½ΠΈΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠ½ΠΈΡ
Π³Π΅Π½ΡΠ² ΠΏΡΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΡΠ²Π°Π½Π½Ρ ΡΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ Π·Π° Π½Π°ΡΠ²Π½ΠΎΡΡΡ Π·Π°Π·Π½Π°ΡΠ΅Π½ΠΈΡ
ΠΌΠ°ΡΡΠΈΠΊΡΡΠ².ΠΠ°ΡΠ΅ΡΡΠ°Π»ΠΈ Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈ. ΠΠ’Π€Π ΠΎΡΡΠΈΠΌΡΠ²Π°Π»ΠΈ Π·Ρ Π·Π±Π°Π³Π°ΡΠ΅Π½ΠΎΡ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΠΌΠΈ ΠΏΠ»Π°Π·ΠΌΠΈ Π· Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½ΡΠΌ Π°ΠΊΡΠΈΠ²Π°ΡΠΎΡΠ° Π·Π³ΠΎΡΡΠ°Π½Π½Ρ SiO2, ΡΡΡΠΏΠ΅Π½Π·ΡΡ ΠΊΠ»ΡΡΠΈΠ½ ΠΠ― β ΡΠ· Ρ
Π²ΠΎΡΡΠΎΠ²ΠΎΠ³ΠΎ Π²ΡΠ΄Π΄ΡΠ»Ρ Ρ
ΡΠ΅Π±ΡΠ° ΡΡΡΡΠ². ΠΡΠ»ΡΡΠΈΠ²ΡΠ²Π°Π½Π½Ρ Π·Π΄ΡΠΉΡΠ½ΡΠ²Π°Π»ΠΈ Π·Π° Π½Π°ΡΠ²Π½ΠΎΡΡΡ ΠΎΠ΄Π½ΠΎΠ³ΠΎ Π· ΡΡΡΠΎΡ
ΠΌΠ°ΡΡΠΈΠΊΡΡΠ² β ΠΠ’Π€Π, Nubiplan Π°Π±ΠΎ ΡΡ
ΡΡΠΌΡΡΡ ΠΏΡΠΎΡΡΠ³ΠΎΠΌ 3, 7 ΡΠ° 14 Π΄ΡΠ± Π·Π° ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΈΡ
ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΈΡ
ΡΠΌΠΎΠ² Π² ΡΠ½ΠΊΡΠ±Π°ΡΠΎΡΡ ΠΠ‘-160 (NΓΌve, Π’ΡΡΡΡΡ). Π‘ΠΏΠΎΡΡΠ΅ΡΠ΅ΠΆΠ΅Π½Π½Ρ Π·Π° ΠΆΠΈΠ²ΠΎΡ ΠΊΡΠ»ΡΡΡΡΠΎΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π² ΠΏΠΎΠ³ΡΠ°Π½ΠΈΡΠ½ΡΠΉ Π· ΠΌΠ°ΡΡΠΈΠΊΡΠ°ΠΌΠΈ Π·ΠΎΠ½Ρ Π² ΠΌΠ΅ΠΆΠ°Ρ
ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ»Ρ Π·ΠΎΡΡ Π· Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½ΡΠΌ ΡΠ½Π²Π΅ΡΡΠΎΠ²Π°Π½ΠΎΠ³ΠΎ ΠΌΡΠΊΡΠΎΡΠΊΠΎΠΏΠ° (Nicon TS100, Π―ΠΏΠΎΠ½ΡΡ). ΠΠΊΡΠΏΡΠ΅ΡΡΡ Ρ
ΠΎΠ½Π΄ΡΠΎΠ³Π΅Π½Π½ΠΈΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠ½ΠΈΡ
Π³Π΅Π½ΡΠ² Ρ ΠΊΡΠ»ΡΡΡΡΡ ΠΊΠ»ΡΡΠΈΠ½ ΠΠ― Π²ΠΈΠ·Π½Π°ΡΠ°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ»ΡΠΌΠ΅ΡΠ°Π·Π½ΠΎΡ Π»Π°Π½ΡΡΠ³ΠΎΠ²ΠΎΡ ΡΠ΅Π°ΠΊΡΡΡ Π·Ρ Π·Π²ΠΎΡΠΎΡΠ½ΠΎΡ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΡΡΡ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΠΈ. ΠΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ ΠΆΠΈΡΡΡΠ·Π΄Π°ΡΠ½ΠΎΡΡΡ ΡΠ° ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΈΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΠΊΠ»ΡΡΠΈΠ½ ΠΠ― ΠΏΡΠΈ ΡΡ
ΠΊΡΠ»ΡΡΠΈΠ²ΡΠ²Π°Π½Π½Ρ ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΡΠ²Π°Π»ΠΎ Π·Π½ΠΈΠΆΠ΅Π½Π½Ρ Π²ΠΌΡΡΡΡ ΠΆΠΈΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΈΡ
Π·ΡΠ°Π·ΠΊΠ°Ρ
. Π£ ΠΊΡΠ»ΡΡΡΡΠ°Ρ
ΡΠ· ΠΠ’Π€Π ΡΠ° ΡΡΠΌΡΡΡΡ ΠΠ’Π€Π Ρ Nubiplant ΠΊΡΠ»ΡΠΊΡΡΡΡ ΠΆΠΈΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ½ΠΎ Π·Π½Π°ΡΡΡΠΎ ΠΏΠ΅ΡΠ΅Π²ΠΈΡΡΠ²Π°Π»Π° ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Ρ ΠΏΠΎΠΊΠ°Π·Π½ΠΈΠΊΠΈ. Π‘ΠΏΠΎΡΡΠ΅ΡΡΠ³Π°Π»ΠΈ Π°Π³ΡΠ΅Π³Π°ΡΡΡ ΠΊΠ»ΡΡΠΈΠ½ Ρ Π·ΠΎΠ½Ρ Π±Π΅Π·ΠΏΠΎΡΠ΅ΡΠ΅Π΄Π½ΡΠΎΠ³ΠΎ ΠΌΠ΅ΠΆΡΠ²Π°Π½Π½Ρ Π· ΠΌΠ°ΡΡΠΈΠΊΡΠ°ΠΌΠΈ Π· Π±ΠΎΠΊΡ Π½Π°Π½Π΅ΡΠ΅Π½Π½Ρ. ΠΠΈΡΠ΅Π»Π΅Π½Π½Ρ ΠΊΠ»ΡΡΠΈΠ½ Π½Π° ΠΏΡΠΎΡΠΈΠ»Π΅ΠΆΠ½ΠΈΠΉ Π±ΡΠΊ ΠΌΠ°ΡΡΠΈΠΊΡΡ ΠΏΡΠΎΡΡΠ³ΠΎΠΌ 14 Π΄ΡΠ± Ρ ΠΆΠΎΠ΄Π½ΠΎΠΌΡ Π· Π΅ΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΈΡ
Π·ΡΠ°Π·ΠΊΡΠ² Π½Π΅ Π²ΡΠ΄Π·Π½Π°ΡΠ΅Π½ΠΎ. Π’Π°ΠΊΠΈΠΌ ΡΠΈΠ½ΠΎΠΌ, ΠΠ’Π€Π, Nubiplant ΡΠ° ΡΡ
ΡΡΠΌΡΡ ΠΌΠΎΠΆΡΡΡ Π²ΠΈΠΊΠΎΠ½ΡΠ²Π°ΡΠΈ Π±Π°ΡβΡΡΠ½Ρ ΡΡΠ½ΠΊΡΡΡ ΡΠΎΠ΄ΠΎ ΡΡΡΠΈΠΌΠ°Π½Π½Ρ ΠΊΠ»ΡΡΠΈΠ½Π½ΠΎΡ ΠΏΠΎΠΏΡΠ»ΡΡΡΡ Π² ΠΏΠ΅Π²Π½ΡΠΉ Π΄ΡΠ»ΡΠ½ΡΡ. ΠΠΊΡΠΏΡΠ΅ΡΡΡ Π³Π΅Π½ΡΠ² COL II, ACAN, GPC3, ANXA3, PTN, MGP ΡΠ° VIM ΠΊΠ»ΡΡΠΈΠ½Π°ΠΌΠΈ ΠΠ― ΠΏΡΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΡΠ²Π°Π½Π½Ρ ΠΏΡΠΎΡΡΠ³ΠΎΠΌ 3 ΡΠ° 7 Π΄ΡΠ± Π·Π° Π½Π°ΡΠ²Π½ΠΎΡΡΡ ΠΠ’Π€Π Ρ ΡΡΠΌΡΡΡ ΠΠ’Π€Π ΡΠ° Nubiplant Π·ΡΠΎΡΡΠ°Π»Π° ΠΏΠΎΡΡΠ²Π½ΡΠ½ΠΎ Π· ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΈΠΌΠΈ Π·ΡΠ°Π·ΠΊΠ°ΠΌΠΈ.ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. ΠΠ°ΡΡΠΎΡΡΠ²Π°Π½Π½Ρ ΠΠ’Π€Π, Nubiplant Π°Π±ΠΎ ΡΡΠΌΡΡΡ ΠΠ’Π€Π Ρ Nubiplant ΠΏΡΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΡΠ²Π°Π½Π½Ρ ΠΊΠ»ΡΡΠΈΠ½ ΠΠ― ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΡΠ²Π°Π»ΠΎ Π²ΡΠ΄ΡΡΡΠ½ΡΡΡΡ Π²ΠΈΡΠ΅Π»Π΅Π½Π½Ρ ΠΊΠ»ΡΡΠΈΠ½ Π½Π° ΠΏΡΠΎΡΠΈΠ»Π΅ΠΆΠ½ΠΈΠΉ Π±ΡΠΊ Π·Π°Π·Π½Π°ΡΠ΅Π½ΠΈΡ
ΠΌΠ°ΡΡΠΈΠΊΡΡΠ² ΠΏΡΠΎΡΡΠ³ΠΎΠΌ ΡΡΡΠΎΠ³ΠΎ ΠΏΠ΅ΡΡΠΎΠ΄Ρ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ (14 Π΄ΡΠ±). ΠΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½Ρ ΠΠ’Π€Π, Nubiplant Π°Π±ΠΎ ΡΡΠΌΡΡΡ ΠΠ’Π€Π Ρ Nubiplant ΡΠΏΡΠΈΡΠ»ΠΎ ΡΠΎΡΠΌΡΠ²Π°Π½Π½Ρ ΠΊΠΎΠ»ΠΎΠ½ΡΠΉ ΠΊΠ»ΡΡΠΈΠ½ Ρ
ΠΎΠ½Π΄ΡΠΎΡΠΈΡΠΎΠΏΠΎΠ΄ΡΠ±Π½ΠΎΡ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΡΡ Ρ Π·ΠΎΠ½Ρ ΠΌΠ΅ΠΆΡΠ²Π°Π½Π½Ρ Π· ΠΌΠ°ΡΡΠΈΠΊΡΠ°ΠΌΠΈ ΡΠ° ΠΏΡΠ΄ΡΡΠΈΠΌΡΠ²Π°Π»ΠΎ ΠΆΠΈΡΡΡΠ·Π΄Π°ΡΠ½ΡΡΡΡ ΠΊΠ»ΡΡΠΈΠ½ ΠΏΡΠΎΡΡΠ³ΠΎΠΌ ΡΡΡΠΎΠ³ΠΎ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π½ΠΎΠ³ΠΎ ΠΏΠ΅ΡΡΠΎΠ΄Ρ. ΠΠ°ΡΡΠΎΡΡΠ²Π°Π½Π½Ρ ΠΠ’Π€Π Ρ ΡΡΠΌΡΡΡ ΠΠ’Π€Π ΡΠ° Nubiplant Π΄Π°Π²Π°Π»ΠΎ Π·ΠΌΠΎΠ³Ρ ΠΏΡΠ΄ΡΡΠΈΠΌΡΠ²Π°ΡΠΈ Π΅ΠΊΡΠΏΡΠ΅ΡΡΡ Ρ
ΠΎΠ½Π΄ΡΠΎΠ³Π΅Π½Π½ΠΈΡ
Π³Π΅Π½ΡΠ² ΠΊΠ»ΡΡΠΈΠ½Π°ΠΌΠΈ ΠΠ― Π² ΠΏΠΎΠ³ΡΠ°Π½ΠΈΡΠ½ΡΠΉ Π· ΠΌΠ°ΡΡΠΈΠΊΡΠ°ΠΌΠΈ Π·ΠΎΠ½Ρ. ΠΡΡΠΈΠΌΠ°Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΈ ΡΠ²ΡΠ΄ΡΠ°ΡΡ ΠΏΡΠΎ ΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½Ρ Π΄ΡΡ ΠΌΠ°ΡΡΠΈΠΊΡΡΠ² Π½Π° ΠΎΡΠ½ΠΎΠ²Ρ Π·Π±Π°Π³Π°ΡΠ΅Π½ΠΎΠ³ΠΎ ΡΡΠΎΠΌΠ±ΠΎΡΠΈΡΠ°ΠΌΠΈ ΡΡΠ±ΡΠΈΠ½Ρ Π½Π° ΠΊΠ»ΡΡΠΈΠ½ΠΈ ΠΠ― ΡΠ° ΠΉΠΎΠ³ΠΎ Π±Π°ΡβΡΡΠ½Ρ ΡΡΠ½ΠΊΡΡΡ, ΡΠΎ Ρ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΈΠΌ Π΄Π»Ρ Π·Π°ΡΡΠΎΡΡΠ²Π°Π½Π½Ρ ΠΠ’Π€Π Π· ΠΌΠ΅ΡΠΎΡ Π·Π°ΠΏΠΎΠ±ΡΠ³Π°Π½Π½Ρ ΡΠΎΡΠΌΡΠ²Π°Π½Π½Ρ ΡΡΠ±ΡΠ΅Π²ΠΎ-ΡΠΏΠ°ΠΉΠΊΠΎΠ²ΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠ²