Snapshot volumetric imaging with engineered point-spread functions

The biological world involves intracellular and intercellular interactions that occur at high speed, at multiple scales and in three dimensions. Acquiring 3D images, however, typically requires a compromise in either spatial or temporal resolution compared to 2D imaging. Conventional 2D fluorescence imaging provides high spatial resolution but requires plane-by-plane imaging of volumes. Conversely, snapshot methods such as light-field microscopy allow video-rate imaging, but at the cost of spatial resolution. Here we introduce 3D engineered point-spread function microscopy (3D-EPM), enabling snapshot imaging of real-world 3D extended biological structures while retaining the native resolution of the microscope in space and time. Our new computational recovery strategy is the key to volumetrically reconstructing arbitrary 3D structures from the information encapsulated in 2D raw EPM images. We validate our technique on both point-like and extended samples, and demonstrate its power by imaging the intracellular motion of chloroplasts undergoing cyclosis in a sample of Egeria densa. Our technique represents a generalised computational methodology for 3D image recovery which is readily adapted to a diverse range of existing microscopy platforms and engineered point-spread functions. We therefore expect it to find broad applicability in the study of rapid biological dynamics in 3D

From bulletins to bullets to blogs and beyond: The Karen’s ongoing communication war

Geff Green focuses on the communication approaches taken by the Karen communities displaced from Burma and who live in diaspora. Apparent control and empowerment provided by new technologies may be illusive. When using media for warfare or perhaps for more innocuous public relations purposes, activists may actually create ‘ammunition’ for opponents. Targeted attacks on specific communities or ‘audiences’ have a high impact by reifying discourse in a devastating way by connecting to lived experience in the victim

Determination Of The Absolute Configuration Of Chiral Secondary Alcohols From Tetra-o-acetylglucosidation-induced 1h Nuclear Magnetic Resonance Shifts

Tetra-O-acetylglucosidation-induced 1H nuclear magnetic resonance shifts were found highly characteristic of the absolute configuration of representative examples of A and S chiral secondary alcohols. The most important chemical shift changes of structurally diagnostic value for the determination of the absolute configuration of alcohols concern the protons of the aglycone attached to the carbons at β-position relative to the oxygen atom. © 1985 American Chemical Society.50244918492