Administration of an inhibitor of hydrogen sulfide (H₂S) synthesis via cystathionine γ-lyase (L-propargylglycine; PAG) exacerbates esophageal injury/inflammation in hyperglycemic rats.

<p>Administration of PAG (25 mg/kg) resulted in a significant increase in the histological score of esophageal injury (panel A). PAG administration also reduced serum IL-10 and increased serum IL-17 levels. The effects of PAG on esophageal damage and serum IL-10 were reversed by co-adminstration of L-tryptophan (L-Trp). An inhibitor of another pathway of H₂S synthesis (CHH; O-carboxymethylhydroxylamine; 20 mg/kg) had no effect on esophageal injury, but produced similar changes to serum IL-10 and IL-17 levels as were seen in PAG-treated rats. Panel C shows the irregular hyperemia, stasis (arrows) and perivascular diapedesis that was observed in rats that on the fructose-supplemented drinking water that were treated with PAG. Panel D illustrates that this treatment also resulted in localized detachments of the epithelium from the basement membrane and destructive changes to the epithelial plate (arrow). X200 (hematoxylin and eosin staining). Bars represent the mean ± SEM of at least 6 rats/group. *p<0.05 versus the vehicle-treated group; ^ψp<0.05 versus the corresponding group not treated with L-tryptophan (one-way ANOVA and Neuman-Keuls test).</p

Treatment of healthy rats with inhibitors of hydrogen sulfide (H₂S) synthesis produces negligible esophageal damage, but significantly alters serum levels of IL-10 and IL-17.

<p>Panel A: Small, but statistically significant changes in the histological score of lower esophageal integrity were observed following administration of either of the inhibitors of H₂S synthesis (PAG, L-propargylglycine and CHH, O-carboxymethylhydroxylamine). The changes induced by the H₂S inhibitors were limited to mild mucosa inflammation. Administration of the inhibitors of H₂S synthesis also resulted in significant decreases in serum IL-10 levels, while the H₂S donor, NaHS, had no effect. Small but significant increases in IL-17 levels were observed following administration of PAG, CHH and NaHS. Bars represent the mean ± SEM of at least 6 rats/group. *p<0.05 versus the vehicle-treated group (one-way ANOVA and Neuman-Keuls test).</p

Administration of an inhibitor of hydrogen sulfide (H₂S) synthesis via cystathionine γ-lyase (L-propargylglycine; PAG) increases esophageal injury/inflammation in hyperglycemic rats subjected to water-immersion stress.

<p>The exacerbation of histological damage by PAG (25 mg/kg) was reversed by co-administration of L-tryptophan (L-Trp). Both inhibitors of H₂S synthesis significantly reduced serum IL-10 and increased serum IL-17, and co-administration of L-Trp diminished these effects. The H₂S donor, NaHS, significantly increased IL-10 and reduced IL-17, and the combination of NaHS and L-Trp produced significantly greater changes in levels of these two cytokines. Bars represent the mean ± SEM of at least 6 rats/group. *p<0.05 versus the vehicle-treated group; ^ψp<0.05 versus the corresponding group not treated with L-Trp (one-way ANOVA and Neuman-Keuls test).</p

Summary of some of the key observations in models of mild, moderate and severe esophagitis in rats, and the effects of an inhibitors of hydrogen sulfide synthesis via cystathionine γ-lyase (L-propargylglycine; PAG).

<p>In healthy rats and in the rats with hyperglycemia- or hyperglycemia+stress-induced esophagitis, acute administration of PAG caused a significant exacerbation of esophageal inflammation. Serum levels of IL-10 decreased with the severity of esophagitis, and this was further enhanced in rats treated with PAG. In contrast, serum levels of IL-17 increased sharply in animal with esophagitis, in parallel with the severity of the disease, and administration of PAG caused further increases in all three models. *p<0.05 versus the corresponding vehicle-treated group (Student’s t-test).</p

Esophageal lesions with signs of nonerosive microscopic esophagitis induced by water-immersion stress after 28 days of consumption of fructose-supplemented drinking water.

<p>Panel A: Pretreatment with an inhibitor of cystathionine γ-lyase (L-propargylglycine; PAG) resulted in extensive esophageal inflammation, with profound submucosal leukocyte infiltration. Panel B: Macroscopic appearance of the lower esophagus of rat treated with PAG, with extensive perivascular hemorrhage. Panel C: Macroscopic appearance of lower esophagus of rat treated with PAG and L-tryptophan, the latter providing a protection against the detrimental effects of PAG.</p