Phyto-extraction ability of Digitaria exilis (Fonio) to heavy metals

Among the different contaminants in the environment, heavy metals are unique due to the fact that they cannot be broken down to non-toxic forms. The research was conducted to determine the remediation potential of Digitaria exilis and to identify the varieties that can tolerate heavy metals contamination. Soil was collected in an Automobile mechanic workshop, Government Reserved Area (GRA) Ado-Ekiti. Five kilograms (5 kg) of soils collected was air dried, sieved and filled into plastic buckets. The experiment was a completely randomized designed with three replicates. Three different accessions of Digitaria exilis namely; Jakah D Iburua (JAK), Dinat D Iburua (DID) and Jiw D Iburau (JIW) were planted. Soils were watered till field moisture capacity. Plant height was determined every two weeks after planting. Soil analysis was carried out to determine the physical and chemical properties before and after the experiment. Heavy metals including Cd, Fe, Zn, Pb, As and Cu were determined in the plant roots and shoots using Atomic Absorption Spectrophotometer (AAS). Accession JAK Bio-accumulated heavy-metals in their root than shoot, consequently, significant high plant height was recorded. However, shoot of accession JAK accumulated 22 % Pb, 46.78% Cu and 37.5% Cd and 20.3% Zn than others whereas accession DID accumulated As than accession JAK. Therefore, accession JAK demonstrated highest phyto-extraction of heavy-metals potentials at the root. Variety JIW has the lowest plant height, root and shoots heavy metals accumulation. Although heavy metals contaminated soil affected the growth of D. exilis, nevertheless accession JAK has the highest productivity under this condition and can be further recommended in phyto-extraction of heavy-metals in polluted sites.Keywords: Phyto-extraction, Digitaria exilis and Heavy-metal

In vivo and in silico toxicity profiling of aerial part of Andrographis paniculata revealed potential to precipitate organs toxicity

The many medicinal properties of Andrographis paniculata have made it an important resource in drug discovery. The commonly used powdered aerial part of A. paniculata is however a subject of abuse occasioned by indiscriminate use, raising safety concerns. In this study, we evaluated the safety profile of the suspension of the powdered aerial part of A. paniculata in male and female rats using single and repeated dose toxicity profiling, and the in silico toxicity profiling of its known phytochemicals, with a view to establishing organs safety. Our results showed significant sex dependent alterations in key haematological and biochemical indices. Significant alteration in liver and kidney histo-architectures were consistent with the observed significant increase in AST/ALT ratio and with the in silico toxicity screening of A. paniculata phytochemicals, including andrographolide, which showed potential for hepatotoxicity, binding of proteins and DNA, and inhibition of hERG II. We conclude that, while taking advantage of the many medicinal benefits of this plant, the unguarded and indiscriminate uses may precipitate organ toxicity, induce cellular alteration and potentiate possible cardiovascular risk