Comparative Assessment of Some Rapid Diagnostic Test (RDT) Kits for Malaria Diagnosis in Ibadan, Nigeria

Background:  Deployment of sound diagnostic deliverables remains a crucial component of malaria control and prevention programme in Africa. This study aims to make a comparative assessment of the efficacy of three famous brands of rapid diagnostic kits (RDT) available in Nigerian market, with the traditional Giemsa staining (microscopic) method, in testing for malaria in endemic zones, also to provide relevant information and guidance to individuals, health care service providers, test kit manufacturers as well as health corporate organizations. Methods: Giemsa microscopy along with RDT kits (Acon, Paracheck and SD Bioline) were carried out on 525 patient samples presumed to present with acute uncomplicated malaria by clinical diagnosis. In addition, the total WBC count and haematocrit were conducted on the blood samples. Results: Out of the 525 samples recruited, three hundred (300) 57.1% were found positive by Giemsa microscopy. SD Bioline had a positivity rate of 260 (49.5%) while Acon and Paracheck trailed behind with 200 (38.1%) and 150 (28.6%) samples respectively. The sensitivity, specificity and efficiency of the three RDT kits were as follows: SD Bioline (86.3%, 99.6%, 92%); Paracheck (50%, 97.7%, 70.4%) and Acon (66.7%, 100%, 80.9%) respectively. Children within the age bracket 0-10 years had the highest malaria positivity rate (F=5.29; p< 0.05). 115 (38.3%) of children in this age group were positive for malaria with Mean PCV of 30.65 ± 0.52 compare to non-malaria control. The dominant malaria species was P. falciparum with 280 (93.3%) cases. However, an appreciable cases of P. falciparum & P. vivax 15 (5.0%) along with P. falciparum & P. malariae 5 (1.7%) co-infections were confirmed. It was also observed that the haematocrit value for individuals correlated inversely with the parasite density (r = -0.78; p< 0.05). Conclusion and Recommendation: Giemsa microscopy method still remains the gold standard for malaria diagnosis in limited resources endemic zones and recommends that imported RDT kits for malaria should be validated before use in developing countries. Keywords: Giemsa Microscopy; Rapid Diagnostic Kits; Parasite Density; Co-Infection; Malaria Contro

Spatio Temporal Land Use Land Cover Change Mapping of Malete Elemere: Implication on Development Planning of Emerging Communities

The use of Ecosystem and Biodiversity mapping, land use land cover change detection has been advocated in preparation of developmental master plan in towns and cities. Noticeable changes have been observed within Malete Elemere community since the establishment of Kwara State University Malete, yet its spatial pattern and socio ecological implication have not been investigated. This work seek to determine and produce land cover land use change map of Malete Elemere over the last 10 years and post 15 year periods through change detection techniques so as to evaluate the impact of the establishment of Kwara State university on the settlement spatial development. Landsat 7 Enhanced Thematic Mapper Plus (ETM+) satellite images of 2005, 2010 and 2015 of the study area were acquired from USGS at spatial resolution of 30 m. Radiometric correction were applied to all the images using radiance modules in Idrisi32 with radiance spectral value set at DN 0 (Lmin) and 255 (Lmax). An unsupervised classification was carried out on the composite images of bands 4,3,2,1 for all the selected years to identify possible maximum spectral reflectance classes, this was followed by supervised classification using training sample from the field survey from which image to image spatio-temporal changes statistics were extracted. To generate a prediction of LULC changes for 2025, Cellular Automata-Markovian transition estimator (CA-Markov) in Idrisi32 was used. Various Kappa statistics was used to evaluate the performance of prediction with an average K statistics of above 0.83 recorded. The result shows that built up area gained an astronomical increase (180%) between 2005 and 2015 while forest lost significantly (34%) within the same periods, with most of the gains occurring in 2010 and 2015 after the establishment of KWASU. By 2025, two Major growth pole centres will emerge along Malete Elemere Axis and one minor in Jenkunu Omoni Axis which will exert a great stress on infrastructural facilities and may create a chaotic condition if left unattended to

Ethnobotanical Survey and Habitat Mapping of Medicinal Plants and Its Implication on Conservation Management in Rural Kwara Communities

Background: In Nigeria, medicinal plants are now being threatened due to increased urbanization, land clearing for farming and over-harvesting from their natural habitats. As such, if such trends continue, some of these medicinal plants might increasingly become not available and in the extreme circumstance be faced with extinction. Methods: An epidemiological descriptive field survey that employed a carefully-structured, closed-ended, interviewer-administered, paper-based questionnaire designed to capture information on the use of medicinal plants as antimalarial and for management of other associated illnesses. We also employed Global Positioning System (Garmin etrex 75) to captures the geo-coordinates of previously identified medicinal plants across the footpath transect at 20 m intervals. A total of twenty-one (21) medicinal plant species were surveyed across five communities with varying numbers per locations. Results: Out of the nine (9) identified traditional healers across the communities, all claimed to have used at least one or combinations of these plants for treatment of malaria. An image classification performed through land cover land use map of the study area revealed six classes: swamp /water bodies, river valley, savanna woodland, degraded woodland, grassland and settlements cluster. Most threatened species such as Aristolochia ringes, Mucuna prurins, Azadirachta indica, Kigelia africana, Citrus limon, Ludwigia suffruticosa, Parkia biglobosa, and Picralima nitida are those found in Malete KWASU campus axis in the degraded woodland and settlement cluster classes. This is due to the high level of forest destruction in the area as a result of growing student population and massive constructions of students’ hostel. We reported that about 60% of original plant cover has been lost between 2005 and 2015. It was observed that availability of surface water bodies played a crucial role in influencing the distribution of identified medicinal plants. The nearest neighbour analysis gave a nearest neighbour index of 0.695 at p=0.000003 and z-score of -4.70314. This shows that the observed random distribution of medicinal plants in the study area was statistically significant. It has been observed that random patterns are usually associated with natural occurrences. The random spatial pattern confirms that these plants have not yet been affected by anthropogenic activities and hence need to be conserved there in the wild. Conclusion: There is need to leverage on conservation of medicinal plants for treating malaria in their natural habitats. Also, the need to ensure sustainable harvesting and other socio-ecological process to ensure these are not threatened to the extreme case of extinction in these communities. In the view of the above, we recommend that KWASU-Malete campus axis be monitored, proper urban planning initiatives implemented and ensure cultivation and preservation of these plants are incorporated into the greening efforts of the Kwara state government in this area