Acanthus montanus: An experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles

<p>Abstract</p> <p>Background</p> <p><it>Acanthus montanus </it>(Nees) T. Anderson (Acanthaceae) is a shrub widespread in Africa, the Balkans, Romania, Greece and Eastern Mediterranean. It is used in African traditional medicine for the treatment of urogenital infections, urethral pain, endometritis, urinary disease, cystitis, leucorrhoea, aches and pains. In southeastern Nigeria, the root is popular and acclaimed highly effective in the treatment of furuncles. This study was undertaken to experimentally evaluate the antimicrobial and anti-inflammatory properties of the root extract as well as its effect on phagocytosis and specific cell-mediated immune response which may underlie the usefulness of the roots in treatment of furuncles.</p> <p>Methods</p> <p>The aqueous root extract (obtained by hot water maceration of the root powder) was studied for effects on the growth of clinically isolated strains of <it>Pseudomonas aeruginosa </it>and <it>Staphylococcus aureus</it>. The anti-inflammatory activity was investigated using acute topical edema of the mouse ear induced by xylene, acute paw edema induced by agar in rats, formaldehyde arthritis in rats, vascular permeability induced by acetic acid in mice and heat- and hypotonicity-induced haemolysis of ox red blood cells (RBCs). Also evaluated were the effects on <it>in vivo </it>leukocyte migration induced by agar, phagocytic activity of macrophages on <it>Candida albicans </it>and specific cell-mediated immune responses (delayed type hypersensitivity reaction (DTHR) induced by sheep red blood cell (SRBC)). The acute toxicity and lethality (LD₅₀) in mice and phytochemical constituents of the extract were also determined.</p> <p>Results</p> <p>The extract moderately inhibited the growth of the test organisms and significantly (<it>P </it>< 0.05) inhibited (57%) topical acute edema in the mouse ear. It significantly (<it>P </it>< 0.05) suppressed the development of acute edema of the rat paw in a non-dose-related manner and was not effective in inhibiting the global edematous response to formaldehyde arthritis. It also inhibited vascular permeability induced by acetic acid in mice and the haemolysis of ox RBCs induced by heat- and hypotonicity. The extract increased total leukocyte and neutrophil counts and caused a significant (<it>P </it>< 0.05) dose-related increase in the total number of macrophages at the 800 mg/kg dose. On phagocytic activity, the extract evoked a significant (<it>P </it>< 0.05) increase in the number of macrophages with ingested <it>C. albicans </it>at 800 mg/kg dose, and significantly (<it>P </it>< 0.05) inhibited DTHR in a dose-related manner. Phytochemical tests on the extract revealed an abundant presence of alkaloids and carbohydrates while saponins, glycosides, and terpenoids occurred in trace amounts. Acute toxicity test established an oral and intraperitoneal LD₅₀greater than 5,000 mg/kg.</p> <p>Conclusion</p> <p>The effectiveness of the root of <it>A. montanus </it>in the treatment of furuncles may largely derive from mobilization of leukocytes to the site of the infection and activation of phagocytic activity as well as suppression of exacerbated immune responses by its constituents. Antimicrobial and anti-inflammatory activities are likely contributory mechanisms. Phytochemical constituents such as alkaloids and carbohydrates may be responsible for these pharmacological activities.</p

Blood chemistry and relative organ weights of rabbits fed neem leaf meal based diets

Two experiments were conducted to determine the effect of neem leaf meal (NLM) based diets on blood and relative organ weight characteristics of rabbits. In the first experiment, 36 rabbit bucks aged 7 to 8 months were distributed into four dietary groups, T1 , T2 T3 and T4 of 9 rabbits using completely randomized design. Each group was replicated 3 times with 3 rabbits per replicate. Rabbits in T1 group were fed diet that contained 0% NLM while those in T2 T3 and T4 groups were fed diets that contained NLM at 5%, 10% and 15%, respectively for 112 days. In the second experiment, 36 rabbit does were used and experimental procedures were laid out as described in experiment 1. Blood samples for analysis, live weight and organ weights were collected from each replicate and data obtained analysed statistically. Higher (p&lt;0.05) kidney weight was obtained for rabbit does on diets T2 T3 and T4 . Bucks on diet T2 recorded significantly (p&lt;0.05) higher pancreas weight than those on diet T1 diet. No significant effect (p&gt;0.05) on haematological parameters was observed among all treatments except for MCV and neutrophils which were significantly (p&lt;0.05) decreased in the groups fed diets T2 and T4 and diets T1 and T3 groups respectively than others. Rabbit bucks and does on diet T1 recorded significantly (p&lt;0.05) higher serum cholesterol values than those on the other diets. Serum glucose value of rabbits on diet T4 was significantly (p&lt;0.05) different from those on the other diets. Serum ALP value of rabbits on diet T1 differed significantly (p&lt;0.05) from those on the other 3 diets with the exception of bucks on diet T4 that had similar value with those on diet T1 . Addition of neem leaf meal in rabbit diets increased serum AST and ALT values but the differences were not significant (p&gt;0.05). It was therefore concluded that up to 5% of neem leaf meal can be included in the diet of pubertal rabbits without any deleterious effects on their organs and blood characteristics.Keywords: Rabbits, neem leaf meal, haematology, serum biochemistry, organ

Growth performance of boilers fed palm oil treated diets supplemented with Vitamin E.

No Abstract.Animal Production Research Advances Vol. 4 (1) 2008: pp. 78-8

Biometric measurements of body and internal organ traits of indigenous chickens on free range in South-Eastern Nigeria

A biometric study of the body conformation and internal organs of the indigenous chickens on free range in rural areas of Imo State in South-Eastern Nigeria was conducted using 5 Local Government Areas. It was observed that the predominant plumage colours were black (51.25%) and brown (36.25%) for the hens while the cocks were predominantly black (30.36%) and red (25.01%). The dressed colour of the birds was majorly yellow and white comprising of (70.68%) and (29.32%) for the hens and (77.75%) and (22.25%) for the cocks, respectively. Shank colour was black (95.4%) and white (4.65%) for the hens, while the cocks were (54.24%) black and (46.76%) white respectively. The mean shank length, neck length and back length of 6.725±0.09 cm, 11.11±0.20 cm and 17.77±0.29 cm were estimated for the hens, while the cocks had mean shank length, neck length and back length of 7.79±0.13 cm, 11.35±0.30 cm and 18.29±0.44 cm, respectively. The mean body weights of the hens and cocks were 868.48±21.18 g, and 1032±57.84 g, respectively with cocks being significantly (P&lt;0.05) higher in body weight and back length than hens. The mean biometric and internal organ measures of chickens per Local Government Area (LGA) were also investigated with no significant (P&gt;0.05) differences among chickens in the five LGAs except for weight of proventriculus and full intestine. Regression equations for the prediction of body weight using the biometric and internal organ measurements were obtained under two functional forms (linear and semi-log) which showed a weak coefficient of determination (R2) ranging between 7.5 to 24.3% for biometric measurements, and a strong coefficient of determination (R2) ranging between 21.4 to 98.89% for internal organs. There was no significant correlation coefficient (r) between the biometric measurements taken, but strong and significant correlation (r) was shown between internal organs and body weight. Therefore, indigenous chickens of South-Eastern Nigeria exhibit wide variability among phenotypes in terms of physical appearance, biometric measurement and internal organ characteristics. More so, the internal organs can be predicted more effectively than biometric measurements by the body weight, using regression models of the two functional forms; and significant indirect selection can be made to improve internal organs like gizzard weight using the bodyweight, due to significant correlation coefficient established.Keywords: - biometric, internal organs, prediction equation, indigenous chicken, extensive, South -Eastern Nigeria