7 research outputs found
The NodD protein does not bind to the promoters of inducible nodulation genes in extracts of bacteroids of Rhizobium leguminosarum biovar viciae
Microbial Biotechnolog
Subcellular localization of the nodD gene product in Rhizobium leguminosarum
Microbial Biotechnolog
Suppression of nodulation gene expression in bacteroids of Rhizobium leguminosarum biovar viciae
Microbial Biotechnolog
Induction of the nodA promoter Rhizobium leguminosarum Sym plasmid pRL1JI by plant flavanones and flavones
An expression vector containing the Rhizobium leguminosarum nodA promoter cloned in front of the Escherichia coli lacZ gene was used to characterize the properties of the R. leguminosarum nodA gene-inducing compound(s) present in sterile root exudate of the host plant Vicia sativa L. subsp. nigra (L.). The major inducing compound was flavonoid in nature, most likely a flavanone. The commercially available flavonoids naringenin (5,7,4'-trihydroxyflavanone), eriodictyol (5,7,3'4'-tetrahydroxyflavanone), apigenin (5,7,4'-trihydroxyflavone), and luteolin (5,7,3',4'-tetrahydroxyflavone) induced the nodA promoter to the same level as the root exudate. On the basis of chromatographic properties, it was concluded that none of these compounds is identical to the inducer that is present in root exudate. The induction of the nodA promoter by root exudate and by the most effective inducer naringenin was very similar, as judged from the genetic requirements and the kinetics of induction.Plant sciencesBio-organic SynthesisMicrobial Biotechnolog
nodO, a new nod gene of the Rhizobium leguminosarum biovar viciae sym plasmid pRL1JI, encodes a secreted protein
Microbial Biotechnolog