Development of osteogenic cell sheets for bone tissue engineering applications

The use of scaffolds in combination with osteogenic cells has been the gold standard in bone tissue engineering strategies. These strategies have, however, in many cases failed to produce the desired results due to issues such as the immunogenicity of the biomaterials used and cell necrosis at the bulk of the scaffold related to deficient oxygen and nutrients diffusion. Here, we originally propose the use of cell sheet (CS) engineering as a possible way to overcome some of these obstacles. Osteogenic CSs were fabricated by culturing rat bone marrow stromal cells in thermoresponsive culture dishes. The CSs were recovered from the dishes using a low-temperature treatment and then were implanted subcutaneously in nude mice. New bone formation was verified from day 7 post-transplantation using X-ray, microcomputed tomography, and histological analysis. The presence of a vascularized marrow was also verified in the newly formed bone after 6 weeks of transplantation. Further, osteocytes were found in this newly formed tissue, supporting the conclusion that mature bone was formed after ectopically transplanting osteogenic CSs. These results therefore confirm the great potentiality of CS engineering to be used in bone tissue engineering applicationsThis study was partially supported by Formation of Innovation Center for Fusion of Advanced Technologies in the Special Coordination Funds for Promoting Science and Technology "Cell Sheet Tissue Engineering Center (CSTEC)" and the Global COE program, Multidisciplinary Education and Research Center for Regenerative Medicine (MER-CREM), from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.Financial support to R. P. Pirraco by the Portuguese Foundation for Science and Technology (FCT) through the PhD grant SFRH/BD/44893/2008 is also acknowledged

Die Reflexion über die Macht der Gewaltlosigkeit: Die Sozialethik der Erinnerung und Versöhnung im Kontext von Hiroshima

広島大学応用倫理学プロジェクト研究センター研究成果報告書統一テーマ : ［平和と和解］ 《Frieden und Versöhnung

Halfbred

This CD-ROM documents a multidisciplinary project presented in 1995 at three Vancouver venues (Grunt, Pitt Gallery and Video In), that included exhibitions (painting, photography, installation, video) and performances by approximately 20 artists, as well as essays (available on the CD-ROM) on issues of bisexuality, miscegenation and transgender. Includes brief texts on each artist’s work. Brief biographical notes