Reproductive Toxicity Potentials of Methanolic Extract of Portulaca Oleracea in Male Rats: an Experimental Study

Background: Purslane is an edible widely distributed shrub and one of the herbs used in decoctions for the treatment of different ailments including infertility. However, there is a shortage of evidence to validate its reproductive effects. Objective: To investigate the effect of methanolic extract of Portulaca oleracea (MEPO) on the reproductive system of male rats. Materials and Methods: Fifteen 10-wk old male Wistar rats with an average weight of 183 gr were randomly divided into three groups (n = 5/each). Group A (the control group) received distilled water only; group B received 400 mg/kg MEPO; and group C received 800 mg/kg MEPO for 14 days. The animals Fasted overnight after the 14th day of administration and euthanized by cervical dislocation. Blood samples, sperm, testes, and epididymis were collected for serum hormones, sperm, and histological analyses. Results: There was no significant change in the serum luteinizing hormone and testosterone levels across all groups when compared to the control. However, group C showed a significant increase (p = 0.020) in follicle-stimulating hormone levels when compared to the control. There was a significant reduction (p = 0.006) in the sperm count in group C when compared with the control group. There was also a significantly reduced (p = 0.003) sperm motility in MEPO-treated groups compared to the control. While the testis showed no abnormalities in its histoarchitecture across groups, the epididymis showed some blood congestion in MEPO-treated groups. Conclusion: Portulaca oleracea showed the ability to reduce sperm count and motility at higher doses. Key words: Portulaca oleracea, Purslane, Testis, Epididymis, Rat, Sperm motility

Health implications of stream water contamination by industrial effluents in the Onitsha urban area of Southeastern Nigeria

Nigeria has abundant surface and ground water resources many of which are polluted and can be detrimental to human health when consumed. This study investigated the effects of effluents discharged by industries into streams on the health of people who depend on stream water for domestic purposes in the Onitsha urban area of eastern Nigeria. Water samples collected from eleven discharge locations underwent physico-chemical and microbiological analyses. Data on the effects of industrial effluents on health were obtained from records in the public hospitals located in Onitsha as well as through questionnaire surveys and field observations. The results of the analyses revealed that the effluents grossly degrade surface water bodies; several parameters (temperature, iron, dissolved oxygen, turbidity, biological oxygen demand, chemical oxygen demand, lead, magnesium, total heterotrophic counts, total coliform group, pH) had values which were higher than the WHO (2011) safety limits for drinking water. The contamination of investigated streams by effluents had negative impact on the health of stream users. The discussion included health effects of polluted water and the prevalence of water borne or related diseases in the area. Implications of these findings were also discussed. Management measures capable of minimizing contamination of surface water in the study area were suggested

Tuberculosis and HIV/AIDS coinfection in patients attending Directly Observed Treatment Short‐course (DOTS) centers in Anambra State, Nigeria: A retrospective study

Background and Aim(S)This study retrospectively assessed the prevalence of TB and human immunodeficiency virus (HIV)/AIDS coinfection among patients that attended the Directly Observed Treatment Short-course (DOTS) centers in Anambra State, Southeast, Nigeria, between 2013 and 2017.MethodsThe study adopted a descriptive and retrospective epidemiological survey design. A total of 1443 case files of patients aged 15−60 who were treated in DOTS centers selected from Anambra State's 21 Local Government Areas between 2013 and 2017 were investigated. The uniform data form, a standardized instrument used in Anambra State's health facilities for data collection, was used to collect data from case files of all those identified as coinfected with TB and HIV/AIDS.ResultsThe mean prevalence rate of TB and HIV/AIDS coinfection in the state during the 5-year period (2013–2017) was 20.00%. The highest annual prevalence of TB and HIV/AIDS coinfection was recorded in 2014 (23.84%). The state's prevalence of TB and HIV/AIDS coinfection increased dramatically from 13.17% in 2013 to 23.84% in 2014, followed by a slight downward trend to 22.80% in 2015, 20.17% in 2016, and 20.03% in 2017. In terms of gender, age, marital status, and occupation, females (59.5%), those aged 15 to 25 years (30.7%), married people (43.90%), and traders/business owners (50.7%), respectively, had the highest rates of tuberculosis and HIV/AIDS coinfection during the study period.ConclusionThe findings of this study show that young people, females, married people, and traders/business owners appear to be the most vulnerable groups affected by TB and HIV/AIDS coinfection, accounting for the majority of the disease burden in the state. To address the high prevalence of TB and HIV/AIDS coinfection in the Anambra State, novel intervention and control programs should be developed and implemented, and existing intervention frameworks should be strengthened