Changes in Salivary Cortisol Concentration in Horses during Different Types of Exercise

This study aimed to estimate the change of stress level in horses based on cortisol concentration levels in their saliva. A total of 61 horses were divided into the following three groups: i) tourist riding experience (TR, n = 23); ii) resting group (RR, n = 14); and iii) horse-riding education (ER, n = 24). The saliva samples of TR and ER groups were taken using plain cotton Salivettes four times a day: at 07:00 (basal), 11:00 (Exercise 1, after 1-hour exercise in the morning), 14:00 (Exercise 2, after 1-hour exercise in the afternoon), and 16:00 (Exercise 3, after 1-hour exercise in the afternoon). The saliva samples of RR were measured at the same time. The samples were analyzed using the SAS program general linear model procedure. In a percentage relative to the base value, cortisol levels in Exercise 3 were confirmed to decrease in all groups as compared to the basal value percentage in the following sequence: ER>TR>RR. The highest peak was confirmed in Exercise 2 (approximately 131%) of RR group and the lowest peak appeared in Exercise 3 (approximately 52%) of ER group. Therefore, resting without any particular exercise can also increase the stress level of horses. Thus, it is better to exercise, as exercise can reduce the stress level, even in cases when riders are clumsy or lack appropriate horse-riding experience. The results of the present study are useful to equestrian center owners and educational riding instructors in that they provide a meaningful insight into a better horse management

Recent Trend in the Incidence of Premalignant and Malignant Skin Lesions in Korea between 1991 and 2006

We evaluated the recent trend in the incidence of premalignant and malignant skin lesions between 1991 and 2006. Among 571,057 newly registered dermatology out-patients from our 8 affiliated university hospitals, 2,598 were diagnosed with a premalignant (899, 0.16%) or malignant skin lesions (1,699, 0.30%). Of 899 premalignant cases, 71.2% were actinic keratosis (AK), and 24.6% were Bowen's disease. Of 1,699 malignant cases, 46.2% were basal cell carcinoma, followed by squamous cell carcinoma (19.1%) and melanoma (7.1%). This 16-yr survey was divided equally into two time periods to compare the incidence of premalignant and malignant skin lesions at different time settings. Between 1991 and 1998, the incidence of cutaneous premalignancy was 0.10% which doubled during 1999-2006. For cutaneous malignancy, the incidence was 0.25% during 1991-1998 and 0.34% in 1999-2006. Incidence of AK among the new outpatients was 0.07% in 1991-1998 which staggered up to 0.15% in 1999-2006. These findings show an increase of both premalignant and malignant skin lesions, AK in particular in the dermatology outpatient-based incidence

Telomerase Activity and the Risk of Lung Cancer

Telomerase play a key role in the maintenance of telomere length and chromosome integrity. We have evaluated the association between telomerase activity and the risk of lung cancer in peripheral blood. Telomerase activity in peripheral blood mononuclear cells was measured by a PCR-designed telomeric repeat amplification protocol in 63 lung cancer patients and 190 healthy controls that were matched for age, gender, and smoking status. Telomerase activity was significantly lower in the lung cancer patients than in controls (mean ± standard deviation; 1.32 ± 1.65 vs 2.60 ± 3.09, P < 1 × 10-4). When telomerase activity was categorized into quartiles based on telomerase activity in the controls, the risk of lung cancer increased as telomerase activity reduced (Ptrend = 1 × 10-4). Moreover, when the subjects were categorized based on the median value of telomerase activity, subjects with low telomerase activity were at a significantly increased risk of lung cancer compared to subjects with high telomerase activity (adjusted odds ratio = 3.05, 95% confidence interval = 1.60-5.82, P = 7 × 10-4). These findings suggest that telomerase activity may affect telomere maintenance, thereby contributing to susceptibility to lung cancer

Influence of Horse and Rider on Stress during Horse-riding Lesson Program

The present study aims to confirm the influence of a horse-riding lesson program (HRLP) on the stress level of horses and riders by respectively analyzing their salivary cortisol concentration. Twenty-four healthy horses and 23 riders participated in this study. The horses were randomly classified into two groups for the horse riding lesson program: Class 1 (for the beginner lesson) and Class 2 (for the intermediate lesson). The Class 1 group consisted of 12 horses and 12 riders, while the Class 2 group consisted of 12 horses and 11 riders. Salivettes cotton wool swabs were used for saliva collection and the saliva analyses were conducted using a two-way analysis of variance for repeated measures with SAS version 8. As for the results, the average salivary cortisol concentration of all horses before HRLP significantly increased compared to the baseline (p<0.001) while it decreased after the HRLP. The results of the salivary cortisol concentration of the riders were similar to the horses’ results. However, there was no difference during the HRLP between Class 1 and Class 2 in the horse or rider groups. The results suggest that the HRLP did not influence the stress level of the horses or riders. Thus, this study provides the necessary information and guidelines for future studies on stress in horses during riding and gives insight into better horse welfare and management options

Effect of age on heart rate, blood lactate concentration, packed cell volume and hemoglobin to exercise in Jeju crossbreed horses

Abstract Background This study aimed to analyze the on heart rate, blood lactate concentration, packed cell volume (PCV) and hemoglobin (Hb) response after conducting exercise in endurance horses. Methods A total of 20 healthy 3\u20139-years-old Jeju crossbreed mares (5.95\u2009\ub1\u20092.24\ua0year) of age and 312.65\u2009\ub1\u200913.59\ua0kg of weight) currently participating the endurance competition were used. The field tests selected for the experiment was gallop (approximately 8.3\ua0m/s) along the selected 2.5\ua0km course (a natural forest trail, not artificial road; a closed loop course). The horses were divided into three groups according to their age; 3\u20134 years of age (G1, 3.29\u2009\ub1\u20090.49\ua0year), 6\u20137 years of age (G2, 6.42\u2009\ub1\u20090.53), and 8\u20139 years of age (G3, 8.50\u2009\ub1\u20090.55). The measurements times for the heart rate, blood lactate concentration, PCV, and Hb analysis were conducted before exercise (T0), shortly after exercise (T1), 15\ua0min after exercise (T2), and 30\ua0min after exercise (T3), respectively. Data was analyzed using an analysis of covariance (ANCOVA) for repeated measures with times and groups. Results The results of the comparison depending on the passage of rest time after exercise suggest that the heart rate and blood lactate concentration of three groups at T2 significantly decreased compared to T1 ( p \u2009<\u20090.001). PCV of the G2 and G3 groups were significantly decreased at T2 compared to T1 ( p \u2009<\u20090.01). Hb values at G2 ( p \u2009<\u20090.01) and G3 ( p \u2009<\u20090.001) groups were significantly decreased at T2 as compared to T1. However, heart rate, blood lactate concentration, PCV and Hb level at T1 showed no difference in the comparison of horses from different age groups with the exception of G3 group in terms of heart rate. Conclusion The physiologic and hematological responses of horses during recovery time after 2,500\ua0m exercise with gallop were no significant difference among the groups. These data are useful as a response evaluation method for training of endurance horses

Physiological Changes in Jeju Crossbred Riding Horses by Swim Training

The changes in physiologic parameters by swim exercise duration were examined in five female well-trained Jeju crossbred riding horses that had riding experience of more than three years without swim training experience. The horses were performed with swim exercise for 10 min (60.0 m/min) once a day for 14 days. Physiologic characteristics and haematic parameters were measured before swimming, immediately after swimming, and after a 10 min rest at first day (D0), 7 days (D7), and 14 days (D14) of training. After 14 days of swim training, heart rate (p<0.05), blood glucose (p<0.05), lactate concentration (p<0.001), packed cell volume (p<0.01), and hemoglobin (p<0.01) measured immediately after swim and after 10 min rest showed significant lower values than those of D0. The results illustrate the benefits of swim training for riding horses and the need for the establishment of swimming routines of appropriate duration and intensity to maximize the advantages of swim training

Filtration Performance Characteristics of Sticky Aerosol Using Calcium Hydroxide

This study examined the performance of removing aerosol upon a flow rate variable by agglomerating sticky aerosol with calcium hydroxide and removing cohesive aerosol through an experimental apparatus, simulating an actual painting booth. As a result of examining the performance of the filter by fixing the paint spray quantity, the calcium hydroxide input and the filtration area under variable flow rates of 5, 10, and 15 Nm3/min, we confirmed that the filter performance has long average aerosol removing intervals at the 5 Nm3/min flow rate. At the 5 Nm3/min flow rate, there is a low residual pressure drop trend and high fractional collection efficiency, and a high level of total collection efficiency is maintained at 99.42%. When the flow rate is less than 5 Nm3/min, the aerosol settling and experimentation was impossible. With this research, the optimal conditions for the use of sticky aerosol have been examined

Serum Alkaline Phosphatase Levels Predict Infection-Related Mortality and Hospitalization in Peritoneal Dialysis Patients.

BACKGROUND:Serum alkaline phosphatase (ALP) levels have been reported to be associated with all-cause and cardiovascular mortality in peritoneal dialysis (PD) patients. However, it is unclear whether serum ALP levels predict infection-related clinical outcomes in PD patients. The aim of this study was to determine the relationships between serum ALP levels, infection-related mortality and hospitalization in PD patients. METHODS:PD patients from the Clinical Research Center registry for end-stage renal disease, a multicenter prospective observational cohort study in Korea, were included in the present study. Patients were categorized into three groups by serum ALP tertiles as follows: Tertile 1, ALP 155 U/L. Tertile 1 was used as the reference category. The primary outcomes were infection-related mortality and hospitalization. RESULTS:A total of 1,455 PD patients were included. The median follow-up period was 32 months. The most common cause of infection-related mortality and hospitalization was PD-related peritonitis. Multivariate Cox regression analyses showed that patients in the highest tertiles of serum ALP levels were at higher risk of infection-related mortality (HR 2.29, 95% CI, 1.42-5.21, P = 0.008) after adjustment for clinical variables. Higher tertiles of serum ALP levels were associated with higher risk of infection-related hospitalization (Tertile 2: HR 1.56, 95% CI, 1.18-2.19, P = 0.009, tertile 3: HR 1.34, 95% CI, 1.03-2.62, P = 0.031). CONCLUSIONS:Our data showed that elevated serum ALP levels were independently associated with a higher risk of infection-related mortality and hospitalization in PD patients