3 research outputs found
Genetic Diversity of Plasmodium falciparum Field Isolates from South Western Nigeria
Background: Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use
of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria.
Methods: In this study, 100 P. falciparum strains merozoite surface protein 1( msp-1), merozoite surface protein 2 (msp-2)
and Glutamate rich protein (Glurp) from Ogun State General Hospitals were characterized. The genetic diversity of P.
falciparum isolates was analyzed by restriction fragment length polymorphism following gel electrophoresis of DNA products
from nested polymerase chain reactions (PCR) of their respective allelic families KI, MAD 20, RO33 (MSP-1);FC27, 3D7
(MSP-2) and Glutamate rich protein respectively.
Results: Majority of the patients showed monoclonal infections while multiplicity of the infection for msp-1 and msp-2 were
1.1 and 1.2 respectively. The estimated number of genotypes was 8 msp-1 (4 KI; 3 MAD; 1 RO33) and 6 msp-2 (3 FC27;
3 3D7). 80% of the isolates coded for Glurp with allelic size ranged between 700 and 900 bp.
Conclusion: The allelic distributions however were similar to those previously reported in other endemic malaria countries.
Future studies will be designed to include other malaria endemic regions of Nigeria such as the oil exploration region
Genetic diversity of Plasmodium falciparum field Isolates from South western Nigeria
Abstract Background: Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria
Genetic diversity of Plasmodium falciparum field isolates from south western Nigeria
Background: Plasmodium falciparum the main causative agent of malaria
is an important public health vector. With the use of PCR, its genetic
diversity has been extensively studied with dearth information from
Nigeria. Methods: In this study, 100 P. falciparum strains merozoite
surface protein 1( msp-1), merozoite surface protein 2 (msp-2) and
Glutamate rich protein (Glurp) from Ogun State General Hospitals were
characterized. The genetic diversity of P. falciparum isolates was
analyzed by restriction fragment length polymorphism following gel
electrophoresis of DNA products from nested polymerase chain reactions
(PCR) of their respective allelic families KI, MAD 20, RO33
(MSP-1);FC27, 3D7 (MSP-2) and Glutamate rich protein respectively.
Results: Majority of the patients showed monoclonal infections while
multiplicity of the infection for msp-1 and msp-2 were 1.1 and 1.2
respectively. The estimated number of genotypes was 8 msp-1 (4 KI; 3
MAD; 1 RO33) and 6 msp-2 (3 FC27; 3 3D7). 80% of the isolates coded for
Glurp with allelic size ranged between 700 and 900 bp. Conclusion: The
allelic distributions however were similar to those previously reported
in other endemic malaria countries. Future studies will be designed to
include other malaria endemic regions of Nigeria such as the oil
exploration regions