Detection of Hepatitis B Virus from Inmates in Correctional Facilities in Niger State

The aim of this study was to determine the prevalence of Hepatitis B virus infection among inmates of selected correctional facilities in Niger state, Nigeria. Blood samples were collected from inmates in three correctional facilities (Bida, Kontagora and Minna). Questionnaires were administered to get their bio-data and 5ml of blood sample was collected from a total of 344 inmates. The plasma was separated and tested for hepatitis B surface antigen (HBsAg) using rapid chromatographic immunoassay test (ICT) and enzyme linked immunosorbent assay test kits (ELISA) . All the HBsAg positive samples were subjected to further test using 5-panel HBV test card. Out of the 344 samples collected, 75 (22%) were positive by ICT for HBsAg. ELISA gave an overall prevalence rate of 25% (87/344) as additional 12 samples were positive. The result of the 5-panel showed that HBsAg, HBsAb, HBeAg, HBeAb and HBcAb were present in 87, 19, 20, 47 and 68 plasma respectively. This implies that 87 persons were infected, 19 had immunity against the virus, 20 had active viral replication, 47 with no viral replication and 68 with onset of acute infection. The HBV infection was highest in the age bracket 21-30 years (29.7%) and lowest in 61-70% (0%). Out of the associated risk factors, sharing of objects showed statistically significant association with the high prevalence of the HBV. This study showed the prevalence of HBV among inmates. As such, there is need for constant screening of the inmates for effective prevention measure and proper clinical management strategy

Clinical validation of cutoff target ranges in newborn screening of metabolic disorders by tandem mass spectrometry: A worldwide collaborative project.

Purpose: To achieve clinical validation of cutoff values for newborn screening by tandem mass 215 spectrometry through a worldwide collaboration. Methods: Cumulative percentiles of amino 216 acids and acylcarnitines in dried blood spots of approximately 30 million normal newborns and 217 10,615 true positive cases are compared to assign clinical significance, which is achieved when 218 the median of a disease range is either >99%ile or <1%ile of the normal population. The cutoff 219 target ranges of analytes and ratios are then defined as the interval between the limits of the two 220 populations. When overlaps occur, adjustments are made to maximize sensitivity and specificity 221 taking in consideration all available factors. Results: As of December 1, 2010, 129 sites in 45 222 countries have uploaded to the project website a total of 23,970 percentile data points, 558,168 223 analyte results of 10,615 true positive cases with 64 conditions, and 5,088 cutoff values. The 224 average rate of submission of true positive cases between December 1, 2008 and December 1, 225 2010 was 4.7 cases per day (3,418 cases). This cumulative evidence generated 91 and 23 high 226 and low target cutoff ranges, respectively. The overall proportion of cutoff values within the 227 respective target range was 43% (2,176/5,088). Conclusions: An unprecedented level of 228 cooperation and collaboration has allowed the objective definition of cutoff target ranges for 114 229 markers applied to newborn screening of rare metabolic disorders. This set of data could be used 230 as baseline for monitoring of future performance

Clinical validation of cutoff target ranges in newborn screening of metabolic disorders by tandem mass spectrometry: A worldwide collaborative project

PURPOSE:: To achieve clinical validation of cutoff values for newborn screening by tandem mass spectrometry through a worldwide collaborative effort. METHODS:: Cumulative percentiles of amino acids and acylcarnitines in dried blood spots of approximately 25-30 million normal newborns and 10,742 deidentified true positive cases are compared to assign clinical significance, which is achieved when the median of a disorder range is, and usually markedly outside, either the 99th or the 1st percentile of the normal population. The cutoff target ranges of analytes and ratios are then defined as the interval between selected percentiles of the two populations. When overlaps occur, adjustments are made to maximize sensitivity and specificity taking all available factors into consideration. RESULTS:: As of December 1, 2010, 130 sites in 45 countries have uploaded a total of 25,114 percentile data points, 565,232 analyte results of true positive cases with 64 conditions, and 5,341 cutoff values. The average rate of submission of true positive cases between December 1, 2008, and December 1, 2010, was 5.1 cases/day. This cumulative evidence generated 91 high and 23 low cutoff target ranges. The overall proportion of cutoff values within the respective target range was 42% (2,269/5,341). CONCLUSION:: An unprecedented level of cooperation and collaboration has allowed the objective definition of cutoff target ranges for 114 markers to be applied to newborn screening of rare metabolic disorders. © 2011 Lippincott Williams &amp; Wilkins