Interspecific hierarchies from aggressiveness and body size among the invasive alien hornet, Vespa velutina nigrithorax, and five native hornets in South Korea.

The range of the invasive alien hornet, Vespa velutina nigrithorax, has been expanding since its introduction to Korea in 2003. Here, we compare the aggressive behaviors and body size of V. velutina nigrithorax with five native hornet species to identify the interspecific hierarchies that influence the rate of spread of this species. Aggressive behaviors were classified into 11 categories, and each interaction was scored as a win, loss, or tie. We found that V. velutina was superior to V. simillima in fights that V. velutina won and showed a high incidence of threatening behavior. V. mandarinia outperformed V. velutina in fights that V. mandarinia won and grappling behavior was common. V. analis was superior to V. velutina in fights that V. analis won and showed a high degree of threatening behavior. V. crabro was superior to V. velutina in fights that V. crabro won and showed a high rate of threatening behavior. V. dybowskii was superior to V. velutina in fights that V. dybowskii won and showed a high incidence of threatening and grappling behaviors. The body size of V. velutina was greater than V. simillima (although not statistically significant) and smaller than all other Vespa species. Therefore, according to this study, the low interspecific hierarchies of V. velutina seem to be a major cause of the slower spread in Korea than in Europe. However, over time, its density has gradually increased within the forest, where it seems to be overcoming its disadvantages and expanding its range, possibly because the large colonies and good flying abilities of this species help it secure food

Two species of Elasmus japonicus Ashmead and Elasmus polistis Burks (Hymenoptera: Eulophidae) reared from nests of Polistes (Hymenoptera: Vespidae) in Korea

Two species of Elasmus (Hymenoptera: Eulophidae) are newly recognized in South Korea: Elasmus japonicus Ashmead and Elasmus polistis Burks. They were reared from the nests of Polistes (Hymenoptera: Vespidae): E. japonicus from Polistes rothneyi koreanus and E. polistis from Polistes snelleni and P. rothneyi koreanus. Both species are biparental and usually have more females than males

Defensive behavior of the invasive alien hornet, Vespa velutina, against color, hair and auditory stimuli of potential aggressors

Background During recent years, invasion of the yellow-legged hornet (Vespa velutina) has occurred in Europe, Korea and Japan, and stinging accidents often occur as some V. velutina nests are in places where humans can reach them. Misleading information regarding precautionary measures for mitigating wasp attacks has only exacerbated the situation. In this study, we sought to identify appropriate countermeasures by analyzing wasp defensive behavior, with a focus on color, hair and auditory stimuli. Methods Defensive behavior was analyzed using video recordings by creating an experimental frame to attach experimental bundles to nine V. velutina nests in Daegu and Gyeongbuk, South Korea. For the color experiment, eight-color and single-color tests were conducted with bundles of eight colors (black, brown, yellow, green, orange, gray, red and white), and the difference in defensive behavior was tested between black hair/hairless and green hair/black hairless configurations. Results When presented simultaneously with bundles of eight different colors, V. velutina showed the greatest and the longest defensive behavior against the black bundle, followed by brown. A similar response was observed in single-color tests. Furthermore, there was no significant difference in the defensive behavior against black hair and black hairless, but the duration of defensive behavior was longer for black hair. A comparison between green hair and black hairless stimuli indicated that wasps are more sensitive to color than to hair texture. Vespa velutina showed no discernible responses when exposed to selected auditory stimuli (human conversation and loud music). Dark colors and dark hair are characteristic features of potential predators, to which wasps are evolutionarily predisposed, and are accordingly likely to provoke strong defensive responses. The results of this study provide scientifically credible information that can be used to base appropriate precautionary measures against wasp attacks

Multi-Odor Discrimination by Rat Sniffing for Potential Monitoring of Lung Cancer and Diabetes

The discrimination learning of multiple odors, in which multi-odor can be associated with different responses, is important for responding quickly and accurately to changes in the external environment. However, very few studies have been done on multi-odor discrimination by animal sniffing. Herein, we report a novel multi-odor discrimination system by detection rats based on the combination of 2-Choice and Go/No-Go (GNG) tasks into a single paradigm, in which the Go response of GNG was replaced by 2-Choice, for detection of toluene and acetone, which are odor indicators of lung cancer and diabetes, respectively. Three of six trained rats reached performance criterion, in 12 consecutive successful tests within a given set or over 12 sets with a success rate of over 90%. Through a total of 1300 tests, the trained animals (N = 3) showed multi-odor sensing performance with 88% accuracy, 87% sensitivity and 90% specificity. In addition, a dependence of behavior response time on odor concentrations under given concentration conditions was observed, suggesting that the system could be used for quantitative measurements. Furthermore, the animals’ multi-odor sensing performance has lasted for 45 days, indicating long-term stability of the learned multi-odor discrimination. These findings demonstrate that multi-odor discrimination can be achieved by rat sniffing, potentially providing insight into the rapid, accurate and cost-effective multi-odor monitoring in the lung cancer and diabetes

Additional file 1: of Survey of medicinal plants in the Khuvsgul and Khangai Mountain regions of Mongolia

List of collected of plant voucher specimens of herbarium by Urgamal (XLSX 46 kb