Variable Selection Method using Apriori Algorithm on Contingency Table

We proposed a new applied method for induction of variable selection on contingency table. This method is the application of Apriori algorithm on variable selection of contigency table with iteraction. We assume that variables are dichotomous variable. We confirm that can be select variable, when minimun support is low level by using AIC on variable selection criterion

iPS細胞に由来する生存未分化細胞の形態学的解析

Induced pluripotent stem (iPS) cells possess pluripotency and self-renewal ability. Therefore, iPS cells are expected to be useful in regenerative medicine. However, iPS cells form malignant immature teratomas after transplantation into animals, even after differentiation induction. It has been suggested that undifferentiated cells expressing Nanog that remain after differentiation induction are responsible for teratoma formation. Various methods of removing these undifferentiated cells have therefore been investigated, but few methods involve morphological approaches, which may induce less cell damage. In addition, for cells derived from iPS cells to be applied in regenerative medicine, they must be alive. However, detailed morphological analysis of live undifferentiated cells has not been performed. For the above reasons, we assessed the morphological features of live undifferentiated cells remaining after differentiation induction as a basic investigation into the clinical application of iPS cells. As a result, live undifferentiated cells remaining after differentiation induction exhibited a round or oval cytoplasm about 12 μm in diameter and a nucleus. They exhibited nucleo-cytoplasmic (N/C) ratio of about 60% and eccentric nuclei, and they possessed partially granule-like structures in the cytoplasm and prominent nucleoli. Although they were similar to iPS cells, they were smaller than live iPS cells. Furthermore, very small cells were present among undifferentiated cells after differentiation induction. These results suggest that the removal of undifferentiated cells may be possible using the morphological features of live iPS cells and undifferentiated cells after differentiation induction. In addition, this study supports safe regenerative medicine using iPS cells.九州保健福祉大学平成29年

PIV Measurement of a Flying Table Tennis Ball

AbstractThere are some reports that the Magnus force becomes negative at some situation in wind tunnel test. If so, there is possibility of a variety of curves using change of the direction of the Magnus force. PIV measurements of a flying table tennis ball were conducted to confirm whether a similar phenomenon was observed in real flight. A high-speed camera with a frame rate of 10k fps was used to capture the instantaneous flow field of the flying ball. The imaging region was 210mm × 210mm. The Reynolds number was approximately 6.5 × 104, which corresponds to a smash in table tennis. A coordinate transformation of the ball's fixed coordinate system captured the wake motion of non-rotating and rotating balls. In the non-rotating condition, the averaged velocity field of the ball was observed to be symmetric, whereas, in the rotating condition, it was asymmetric, which shows the Magnus effect. At spin parameter is 0.65, the Magnus force becomes zero to indicate the appearance of the negative Magnus force. These observations quantitatively agree with the wind tunnel test

Mammalian Lgl Forms a Protein Complex with PAR-6 and aPKC Independently of PAR-3 to Regulate Epithelial Cell Polarity

AbstractBackground: Epithelial cells have apicobasal polarity and an asymmetric junctional complex that provides the bases for development and tissue maintenance. In both vertebrates and invertebrates, the evolutionarily conserved protein complex, PAR-6/aPKC/PAR-3, localizes to the subapical region and plays critical roles in the establishment of a junctional complex and cell polarity. In Drosophila, another set of proteins called tumor suppressors, such as Lgl, which localize separately to the basolateral membrane domain but genetically interact with the subapical proteins, also contribute to the establishment of cell polarity. However, how physically separated proteins interact remains to be clarified.Results: We show that mammalian Lgl competes for PAR-3 in forming an independent complex with PAR-6/aPKC. During cell polarization, mLgl initially colocalizes with PAR-6/aPKC at the cell-cell contact region and is phosphorylated by aPKC, followed by segregation from apical PAR-6/aPKC to the basolateral membrane after cells are polarized. Overexpression studies establish that increased amounts of the mLgl/PAR-6/aPKC complex suppress the formation of epithelial junctions; this contrasts with the previous observation that the complex containing PAR-3 promotes it.Conclusions: These results indicate that PAR-6/aPKC selectively interacts with either mLgl or PAR-3 under the control of aPKC activity to regulate epithelial cell polarity