エノキの組織培養と不定根の誘導

エノキ (Celtis sinensis Pers. var, japonica Nakai) の成木の新枝よりナフタレン酢酸 (NAA) 1. 0mg/ℓ, 6-ベンジルアミノプリン (BAP) 0. 1mg/ℓを含むイオン強度を半分にした改変 Murashige-Skoog (MS) 培地を用い緑色カルスを誘導した。このカルスの生育は早く, プランテックスを用いて25℃で2, 500luxの照光下培養した場合, 9週間で絶乾重量が9倍になった。このカルスを0. 5mg/ℓのNAAと1. 0mg/ℓのBAPを含む改変MS培地を用いて暗黒下培養したところ, 67％の率で根が誘導された。A green compact callus was induced from sterilized shoots of Japanese hackberry (Celtis sinensis Pers. var. japonica Nakai) on a Murashige-Skoog medium supplemented with 1.0mg/ ℓ 1-naphthaleneacetic acid (NAA) and 0.1 mg/ ℓ 6-benzylaminopurine (BAP). The callus grew fast on this medium in PLANTEX to attain 9 fold amount of dry weight at 9 weeks incubation at 25℃ with a continuous illumination of 2, 500 lux. Roots were regenerated from the callus after incubation for 4 weeks under dark at hormonal combination of 0.5mg/ ℓ NAA and 1.0 mg/ ℓ BAP at a relatively high frequency of 67%

A transcriptional timetable of autumn senescence

BACKGROUND: We have developed genomic tools to allow the genus Populus (aspens and cottonwoods) to be exploited as a full-featured model for investigating fundamental aspects of tree biology. We have undertaken large-scale expressed sequence tag (EST) sequencing programs and created Populus microarrays with significant gene coverage. One of the important aspects of plant biology that cannot be studied in annual plants is the gene activity involved in the induction of autumn leaf senescence. RESULTS: On the basis of 36,354 Populus ESTs, obtained from seven cDNA libraries, we have created a DNA microarray consisting of 13,490 clones, spotted in duplicate. Of these clones, 12,376 (92%) were confirmed by resequencing and all sequences were annotated and functionally classified. Here we have used the microarray to study transcript abundance in leaves of a free-growing aspen tree (Populus tremula) in northern Sweden during natural autumn senescence. Of the 13,490 spotted clones, 3,792 represented genes with significant expression in all leaf samples from the seven studied dates. CONCLUSIONS: We observed a major shift in gene expression, coinciding with massive chlorophyll degradation, that reflected a shift from photosynthetic competence to energy generation by mitochondrial respiration, oxidation of fatty acids and nutrient mobilization. Autumn senescence had much in common with senescence in annual plants; for example many proteases were induced. We also found evidence for increased transcriptional activity before the appearance of visible signs of senescence, presumably preparing the leaf for degradation of its components

ポプラエンド-1,4-β-グルカナーゼの分子生物学的解析

京都大学0048新制・課程博士博士(農学)甲第8442号農博第1126号新制||農||801(附属図書館)学位論文||H12||N3399(農学部図書室)UT51-2000-F346京都大学大学院農学研究科林産工学専攻(主査)教授 酒井 富久美, 教授 藤田 稔, 教授 東 順一学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDA

<Preliminary>Endo-1,4-β-glucanase in Suspension-cultured Poplar Cells

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Individual and combining effects of anti-RANKL monoclonal antibody and teriparatide in ovariectomized mice

We examined the individual and combined effects of teriparatide and anti-RANKL (receptor activator of nuclear factor κB ligand) monoclonal antibody in ovariectomized mice. Three-month-old female C57BL/6 mice were ovariectomized (OVX) or sham operated. Four weeks after OVX, they were assigned to 3 different groups to receive anti-RANKL monoclonal antibody (Ab) alone (5 mg/kg single injection at 4 weeks after OVX, Ab group), teriparatide alone (80 μg/kg daily injection for 4 weeks from 4 weeks after OVX, PTH group), or mAb plus teriparatide (Ab + PTH group). Mice were sacrificed 8 weeks after OVX. Bone mineral density (BMD) was measured at the femur and lumbar spine. Hind limbs were subjected to histological and histomorphometric analysis. Serum osteocalcin and CTX-I levels were measured to investigate the bone turnover. Compared with Ab group, Ab + PTH group showed a significant increase in BMD at distal femur and femoral shaft. Cortical bone volume was significantly increased in PTH and Ab + PTH groups compared with Ab group. Bone turnover in Ab + PTH group was suppressed to the same degree as in Ab group. The number of TRAP-positive multinucleated cells was markedly reduced in Ab and Ab + PTH groups. These results suggest that combined treatment of teriparatide with anti-RANKL antibody has additive effects on BMD in OVX mice compared with individual treatment