Expression analysis of <i>S-ELF3</i> transcripts.

<p>cDNA prepared from roots, stems, leaves, pistils, stamens and pollen was used for RT-PCR analysis. <i>ACTIN</i> was amplified as a loading control. DBF: one day before flowering. DF: the day of flowering.</p

Expression of genes selected by in <i>silico</i> subtraction as determined by RT-PCR.

<p>The expression of genes corresponding to the 15 contigs selected by <i>in silico</i> subtraction was examined by RT-PCR using cDNA from long styles (LS) and short styles (SS) as templates. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031264#pone.0031264.s007" target="_blank">Table S1</a> for RT-PCR primers.</p

PCR survey of <i>S-ELF3</i> (<i>SSG3</i>) in 47 buckwheat landraces and modern cultivars.

<p>The numbering of individual plants corresponds to that shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031264#pone.0031264.s008" target="_blank">Table S2</a>. L, long-styled plant. S, short-styled plant. N, negative control. M, 1-kb DNA ladder (GenDireX).</p

Dimorphic flowers of buckwheat and schematic presentation of the intra-morph incompatibility response in buckwheat.

<p>Short-styled flowers of buckwheat have long stamens and vice versa. A pollen grain from a long-styled plant germinates and the pollen tube successfully elongates to reach the ovary in the pistil of a short-styled plant, whereas it germinates but fails to elongate in the style of long-styled flower.</p

<i>S-ELF3</i> in <i>Fagopyrum</i> species.

<p>The gene structure and phylogeny of <i>S-ELF3</i> in five <i>Fagopyrum</i> species, including the SC Kyushu PL4 line, which contains the <i>S^h</i> allele of <i>F. homotropicum</i>, are shown. Species in blue and red font exhibit heteromorphic SI and homomorphic SC, respectively. Dark brown boxes and lines represent 5′- and 3′-untranslated regions and introns, respectively. Coding regions are colored blue. Red boxes and line indicate large insertions (>400 bp) and nonsense mutation, respectively. The phylogenetic tree in the inset was obtained by the Neighbor-joining method. The <i>S-ELF3</i> sequence from <i>F. urophyllum</i> was used as an outgroup. The bootstrap numbers (500 replicates) are shown next to the branches. The scale bar corresponds to 0.02 substitution per nucleotide site.</p