Lab-on-foil based portable µPCR for POC nucleic acid testing of HIV-1

We present a low-cost μPCR platform using a thin film microheater on foil, fabricated in only one metallization step using roll-to-roll fabrication. The μPCR module was successfully applied for amplification of HIV-1 viral load in three clinical samples of which one was negative control. The μPCR module was able to amplify the target in the two HIV positive samples whereas the COBAS TaqMan HIV-1 from Roche and an in-house developed qPCR assay using TaqMan probes could not. These results indicate that the μPCR module has the potential to be very sensitive. The low cost μPCR system opens up for the possibility to perform molecular diagnostics at the POC - ideally suited at low-resource settings

Dřevostavba pro rekreační účely

The bachelor thesis deals with the design of a wooden building, which will be used for recreational purposes, and it will also be inhabitable for the whole year. The introductory part focuses on already realized wood buildings for recreational purposes. The architectural design of the recreational building is devised in the theoretical part, including a detailed description of the selected structural system and a description of materials, which will be used for the aforementioned building. For the layers of perimeter construction, there is an assessment of the thermal protection aspect. At the end of the thesis, economic aspects are shown. The thesis also contains a technical report, an accompanying report and basic drawings with added drawings of specified structural details