Dynamic Axonal Translation in Developing and Mature Visual Circuits.

Local mRNA translation mediates the adaptive responses of axons to extrinsic signals, but direct evidence that it occurs in mammalian CNS axons in vivo is scant. We developed an axon-TRAP-RiboTag approach in mouse that allows deep-sequencing analysis of ribosome-bound mRNAs in the retinal ganglion cell axons of the developing and adult retinotectal projection in vivo. The embryonic-to-postnatal axonal translatome comprises an evolving subset of enriched genes with axon-specific roles, suggesting distinct steps in axon wiring, such as elongation, pruning, and synaptogenesis. Adult axons, remarkably, have a complex translatome with strong links to axon survival, neurotransmission, and neurodegenerative disease. Translationally co-regulated mRNA subsets share common upstream regulators, and sequence elements generated by alternative splicing promote axonal mRNA translation. Our results indicate that intricate regulation of compartment-specific mRNA translation in mammalian CNS axons supports the formation and maintenance of neural circuits in vivo.This work was supported by Wellcome Trust Programme Grant (085314/Z/08/Z), European Research Council Advanced Grant (322817) to CEH , Cambridge Wellcome Trust PhD programme in Developmental Biology (PMAG/406; BT-B), Gates Cambridge Scholarship (JQL), Basic Science Research Program (2013R1A1A1009625 & 2014K2A7A1036305), Biomedical Technology Development Program (2013M3A9D5072551), & Brain Research Program (2015M3C7A1028396) funded through the NRF by the Korean government (MSIP), Yonsei University Future-leading Research Initiative of 2015 (2015-22-0095), and a faculty research grant from Yonsei University College of Medicine for 2013 (6-2013-0064-2-1) to HJ.This is the author accepted manuscript. It is currently under an indefinite embargo pending publication by Cell Press

TAIL-seq for X. laevis wild-type early embryos replicate set #1 (internal ID: hs27, part 5/12)

<p>This dataset contains the raw sequencing data from a TAIL-seq run for Xenopus laevis embryos. The cluster intensities of fluorescence signals are repacked as an HDF5 formatted file, then split into multiple parts to fit in the dataset size limitation of the Zenodo.</p

Inhibitory Effect and Mechanism on Antiproliferation of Isoatriplicolide Tiglate (PCAC) from &lt;em&gt;Paulownia Coreana&lt;/em&gt;

&lt;em&gt;Paulownia coreana&lt;/em&gt; has traditionally been used as the medicine and health food in the treatment of cancer and infectious diseases. In the present study, a new antiproliferation agent, isoatriplicolide tiglate (PCAC) was isolated from the chloroform soluble fraction of the leaves of &lt;em&gt;Paulownia coreana&lt;/em&gt;. The antiproliferation activities of PCAC plant extract was examined in breast and cervical cancer cell lines in a time-and dose-dependent manners. Our &lt;em&gt;in vitro&lt;/em&gt; experiments showed that PCAC suppresses the cell growth and proliferation of cancer cells at a relatively low concentration ( &lt; 10 µg/mL) and induces apoptosis at a high concentration ( &gt; 50 µg/mL). Western blot analysis showed that concentration higher than 50 µg/mL induces a time-dependent increase in the percentage of apoptotic cells. In this case, PCAC uses both extrinsic and intrinsic pathways for the apoptosis. PCAC treatment decreased the expression of pro-caspase 8, 9, and 3, the main regulators of apoptotic cell death, in MDA-MB-231 cells, accompanied by the activation of caspase 8, 9, and 3. More importantly, PCAC inhibited the &lt;em&gt;in vitro&lt;/em&gt; proliferation of six other human breast and cervical cancer cell lines. In conclusion, our data strongly suggest that PCAC acts as an antiproliferation agents particularly against breast and cervical cancers by inducing cell cycle arrest in the S/G2 phase and caspase dependent apoptosis at relatively low ( &lt; 10 μg/mL) and high ( &gt; 50 µg/mL) concentrations, respectively

TAIL-seq for X. laevis wild-type early embryos replicate set #1 (internal ID: hs27, part 1/12)

<p>This dataset contains the raw sequencing data from a TAIL-seq run for Xenopus laevis embryos. The cluster intensities of fluorescence signals are repacked as an HDF5 formatted file, then split into multiple parts to fit in the dataset size limitation of the Zenodo.</p

TAIL-seq for X. laevis wild-type early embryos replicate set #1 (internal ID: hs27, part 10/12)

<p>This dataset contains the raw sequencing data from a TAIL-seq run for Xenopus laevis embryos. The cluster intensities of fluorescence signals are repacked as an HDF5 formatted file, then split into multiple parts to fit in the dataset size limitation of the Zenodo.</p

TAIL-seq for X. laevis wild-type early embryos replicate set #1 (internal ID: hs27, part 4/12)

<p>This dataset contains the raw sequencing data from a TAIL-seq run for Xenopus laevis embryos. The cluster intensities of fluorescence signals are repacked as an HDF5 formatted file, then split into multiple parts to fit in the dataset size limitation of the Zenodo.</p

TAIL-seq for X. laevis wild-type early embryos replicate set #1 (internal ID: hs27, part 6/12)

<p>This dataset contains the raw sequencing data from a TAIL-seq run for Xenopus laevis embryos. The cluster intensities of fluorescence signals are repacked as an HDF5 formatted file, then split into multiple parts to fit in the dataset size limitation of the Zenodo.</p

TAIL-seq for X. laevis wild-type early embryos replicate set #1 (internal ID: hs27, part 3/12)

<p>This dataset contains the raw sequencing data from a TAIL-seq run for Xenopus laevis embryos. The cluster intensities of fluorescence signals are repacked as an HDF5 formatted file, then split into multiple parts to fit in the dataset size limitation of the Zenodo.</p