A Woods-Saxon Optical-Model Description of 14N Elastic and Inelastic Scattering from 27Al and 28Si

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Systematic Two-Hybrid and Comparative Proteomic Analyses Reveal Novel Yeast Pre-mRNA Splicing Factors Connected to Prp19

Prp19 is the founding member of the NineTeen Complex, or NTC, which is a spliceosomal subcomplex essential for spliceosome activation. To define Prp19 connectivity and dynamic protein interactions within the spliceosome, we systematically queried the Saccharomyces cerevisiae proteome for Prp19 WD40 domain interaction partners by two-hybrid analysis. We report that in addition to S. cerevisiae Cwc2, the splicing factor Prp17 binds directly to the Prp19 WD40 domain in a 1∶1 ratio. Prp17 binds simultaneously with Cwc2 indicating that it is part of the core NTC complex. We also find that the previously uncharacterized protein Urn1 (Dre4 in Schizosaccharomyces pombe) directly interacts with Prp19, and that Dre4 is conditionally required for pre-mRNA splicing in S. pombe. S. pombe Dre4 and S. cerevisiae Urn1 co-purify U2, U5, and U6 snRNAs and multiple splicing factors, and dre4Δ and urn1Δ strains display numerous negative genetic interactions with known splicing mutants. The S. pombe Prp19-containing Dre4 complex co-purifies three previously uncharacterized proteins that participate in pre-mRNA splicing, likely before spliceosome activation. Our multi-faceted approach has revealed new low abundance splicing factors connected to NTC function, provides evidence for distinct Prp19 containing complexes, and underscores the role of the Prp19 WD40 domain as a splicing scaffold

A Natural Framework for Solar and 17 keV Neutrinos

Motivated by recent experimental claims for the existence of a 17 keV neutrino and by the solar neutrino problem, we construct a class of models which contain in their low-energy spectrum a single light sterile neutrino and one or more Nambu-Goldstone bosons. In these models the required pattern of breaking of lepton-number symmetry takes place near the electroweak scale and all mass heirarchies are technically natural. The models are compatible with all cosmological and astrophysical constraints, and can solve the solar neutrino problem via either the MSW effect or vacuum oscillations. The deficit in atmospheric muon neutrinos seen in the Kamiokande and IMB detectors can also be explained in these models.Comment: 23 page