2 research outputs found
Supplement 1: Pupil function engineering for enhanced nanoparticle visibility in wide-field interferometric microscopy
Originally published in Optica on 20 February 2017 (optica-4-2-247
Label-Free and High-Throughput Detection of Biomolecular Interactions Using a Flatbed Scanner Biosensor
Fluorescence based
microarray detection systems provide sensitive
measurements; however, variation of probe immobilization and poor
repeatability negatively affect the final readout, and thus quantification
capability of these systems. Here, we demonstrate a label-free and
high-throughput optical biosensor that can be utilized for calibration
of fluorescence microarrays. The sensor employs a commercial flatbed
scanner, and we demonstrate transformation of this low cost (∼100
USD) system into an Interferometric Reflectance Imaging Sensor through
hardware and software modifications. Using this sensor, we report
detection of DNA hybridization and DNA directed antibody immobilization
on label-free microarrays with a noise floor of ∼30 pg/mm<sup>2</sup>, and a scan speed of 5 s (50 s for 10 frames averaged) for
a 2 mm × 2 mm area. This novel system may be used as a standalone
label-free sensor especially in low-resource settings, as well as
for quality control and calibration of microarrays in existing fluorescence-based
DNA and protein detection platforms