Antibiotics resistance of a strain of Escherichia coli isolated from bore hole in Ile Ife, Osun state, Nigeria

Abstract:Escherichia coli were isolated from water from two boreholes in Ile Ife, Osun state, Nigeria. This was an indication of faecal contamination. These strains of Escherichia coli were Gram negative short rods, Catalase positive, Methyl red positive, Voges Proskaeur negative. The strains could ferment glucose galactose, sucrose, lactose, mannitol and maltose with the production of acid and gas but could not hydrolyze starch. A particular strain was resistant to sulfamethoxazole, ampicillin, cotrimoxazole, cephaloridine, streptomycin, carbenicillin, sulfafurazole and tetracycline but sensitive to gentamicin, colistin, nalidixic acid, nitrofurantoin and colistin sulphat

In vitro Degradation of Extracted Cassava Linamarin by Bacillus Species Isolated from cassava wastewater

Linamarin is the most abundant cyanoglucoside present in cassava cells and may generate the equivalent amount of hydrocyanic acid. This study was aimed to assess degradative capacities of Bacillus pumilus strain WOB3 and WOB7on linamarin. The test organisms for linamarase activity were identified on the basis of phenotype, biochemical properties and 16S rDNA gene sequencing as: Bacillus pumilus strain WOB3 KX774195 and Bacillus pumilus strain WOB7 KX774196. Growth studies showed that the strains grew in all the substrates tested. The doubling times of Bacillus pumilus strain WOB3 and Bacillus pumilus strain WOB7 were 8.25 d and 7.53 d on cassava effluent, 6.30 dand 5.78 d on supplemented cassava effluent, 8.66 dand 9.90 d on waste leachate and 6.30 d and 9.24 d on supplemented waste leachate respectively; with specific growth rates of 0.084 d-1 and 0.092 d-1 on cassava effluent, 0.11 d-1 and 0.12 d-1 on supplemented cassava effluent, 0.080 d-1 and 0.070 d-1 on waste leachate and 0.11 d-1 and 0.075 d-1 on supplemented waste leachate respectively. High-performance liquid chromatographic analysis studies revealed that linamarin degradation by the strains followed a linamarase pathway involving CO2 and HCN as metabolic intermediates. Based on HPLC analysis, linamarin residual concentration at day 12 by the strains WOB3 and WOB7 was 26.73 mgL-1(19.79 %) and 29.79 mgL-1 (21.92 %). These novel features make the bacteria suitable candidates for in-situ application on sites contaminated with cassava processing wastes

Assessment of apolipoproteins and C-reactive protein as risk predictors of atherosclerosis in type 2 diabetes mellitus

No Abstrac

Growth and distribution patterns of bacterial pathogens recovered from ready-to-eat (RTE) fruit salad from food vendors in Lagos, Nigeria.

The bacteria associated with contamination of ready-to-eat (RTE) fruit salad samples obtained from four markets namely Yaba, Ikeja, Mushin and Oyingbo in Lagos, Nigeria were studied using standard microbiological methods. The objective of this study was to investigate the distribution of pathogenic bacteria recovered from RTE fruit salad in Lagos and assess bacteria load. The total aerobic and fecal coliform counts ranged from 1.50±0.5 x 105 cfu/g to 1.52±0.04 x 109 cfu/g and 5.0±0.5 x 104 cfu/g to 1.49±0.09x108 cfu/g respectively. The isolates were identified using morphological and biochemical tests. The results were analyzed using the one-way-ANOVA test. The test revealed that the average bacteria and coliform counts were not significantly different for the four markets, respectively. A further pairwise test also revealed that there was no significant difference between the markets. The predominant pathogenic bacteria associated with contamination of the RTE fruit salad were identified as E.coli (WO1); Klebisella sp (WO2); Proteus sp (WO3); Pseudomonas sp (WO4); Morganella morganni (WO5); Enterobacter sp (WO6); Staphylococcus aureus (coagulase +) (WO7) and Staphylococcus sp (coagulase-) (WO8). The presence of coliform and other bacteria in numbers exceeding the recommended microbiological standard is a reflection of unwholesome product, hence the need for proper microbiological safety analysis of fruit salad samples prepared for human consumption.Keyword: Contamination, pathogenic, bacteria, ready-to-eat (RTE), fruit salad