Isolation and characterization of pyropheophorbide-a from moringa oleifera lam

Moringa oleifera is a plant rich in pharmacologically active compounds. This study investigated the phytochemical constituents of Moringa oleifera leaf extracts. Dried M. oleifera leaves were ground and extracted successively with hexane, ethyl acetate and methanol using a Soxhlet apparatus. Different chromatographic techniques were used to fractionate the ethyl acetate extract. Thin layer chromatography was used to pool similar fractions together. Fractions obtained were purified using sephadex glass column. The structures of isolated compounds were identified using nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and comparison with published data. Phytochemical screening revealed the presence of flavonoids, terpenoids, carbohydrates and phenols. Ethyl acetate extract which was subjected to column chromatography resulted to the isolation of two compounds: mono acetyl glycerol and pyropheophorbide-a, from the leaves of M. oleifera. This is the first report on the isolation of pyropheophorbide-a from the leaves of M. oleifera

Characterization and Experimental and Computational Assessment of Kola nitida Extract for Corrosion Inhibiting Efficacy

Kola nitida (KN) extract was assessed for its ability to hinder corrosion of mild steel in 0.1 M HCl solution by means of gravimetric and electrochemical tests. KN extract was found to efficiently retard mild steel corrosion in this system, with the effect becoming more pronounced as KN extract concentration increased and slightly subdued as immersion time lengthened. From the polarization and impedance results, KN extract was seen to affect both the cathodic and the anodic reactions following adsorption of organic species from the extract on the metal/solution interface. The presence of such organics was confirmed by UV–visible spectrophotometry and gas chromatography–mass spectroscopy analyses. Selected constituents of the extract were modeled in order to assess their adsorbability using the density functional theory (DFT) and revealed remarkably high interaction energies, which corroborate the experimental findings