Differently Prepared PbO2/Graphitic Carbon Nitride Composites for Efficient Electrochemical Removal of Reactive Black 5 Dye

In this paper, electrochemical degradation of Reactive Black 5 (RB5) textile azo dye was examined in regard to different synthesis procedures for making PbO2–graphitic carbon nitride (g-C3N4) electrode. The reaction of with ClO− in the presence of different surfactants, i.e., cetyltrimethylammonium bromide (CTAB) and tetrabutylammonium phosphate (TBAP), under conventional conditions, resulted in the formation of PbO2 with varying morphology. The obtained materials were combined with g-C3N4 for the preparation of the final composite materials, which were then characterized morphologically and electrochemically. After optimizing the degradation method, it was shown that an anode comprising a steel electrode coated with the composite of PbO2 synthesized using CTAB as template and g-C3N4, and using 0.15 M Na2SO4 as the supporting electrolyte, gave the best performance for RB5 dye removal from a 35 mg/L solution. The treatment duration was 60 min, applying a current of 0.17 A (electrode surface 4 cm2, current density of 42.5 mA/cm2), while the initial pH of the testing solution was 2. The reusability and longevity of the electrode surface (which showed no significant change in activity throughout the study) may suggest that this approach is a promising candidate for wastewater treatment and pollutant removal

Supplementary material for the article: Ognjanović, M.; Dojčinović, B.; Fabián, M.; Stanković, D. M.; Mariano, J. F. M. L.; Antić, B. Microwave Assisted Hydrothermal Synthesis of (Fe,Co)3O4 Nanoparticles in the Presence of Surfactants and Effects of Co/Fe Ratio on Microstructure and Magnetism. Ceramics International 2018, 44 (12), 13967–13972. https://doi.org/10.1016/j.ceramint.2018.04.246

Supplementary material for: [https://doi.org/10.1016/j.ceramint.2018.04.246]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2170]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3236

Paracetamol-induced changes of haemato-biochemical and oxidative stress parameters in rat blood: protective role of vitamin C and betaglucan

Paracetamol (acetaminophen) is widely used as an ov er-the-counter analgesic and antipyretic drug. The aim of this stu dy was to investigate the possible protective effects of vitamin C (100mg/kg/day i.p.) and β -glucan (40 mg/kg/day i.p.) on altered haematological, biochemical and oxidative s tress parameters in the blood of rats treated with paracetamol (100 mg/kg/day i.p.) for 3 days. Exposure of rats to paracetamol caused changes of some haematological parameters (R BCs count, Hb concentration, Ht value and WBCs count), suggesting that the paraceta mol induced haematotoxicity. Paracetamol reduced serum total protein (TP), album in and globulin, while increased alanine aminotransferase (ALT), aspartate aminotran sferase (AST) and lactate dehydrogenase (LDH) activities compared to the cont rol. The results indicate that paracetamol is led to significant decrease in the c oncentration of Na + and K + and increase of Ca 2+ in the serum compared to the control. Coadministra tion of vitamin C and β -glucan with paracetamol reversed these changes of haematol ogical and biochemical parameters and diminished the toxic effects of paracetamol. Th e obtained results indicated that the concentration of LPO in erythrocytes significantly increased in, while the concentration of GSH significantly decreased in the group treated with paracetamol compared to control group. Coadministration of vitamin C and β -glucan with paracetamol reversed paracetamol-induced alterations in these oxidative stress parameters. This study suggests that paracetamol has significant prooxidative effec ts and may disrupt oxidant/antioxidant balance in erythrocytes. Furthermore, coadministrat ion with vitamin C and β -glucan have protective effects on paracetamol-induced oxidative damage and haematotoxicity.Kragujevac Journal of Science (2016), 38: 135-14

Lipid Peroxidative Damage on Cisplatin Exposure and Alterations in Antioxidant Defense System in Rat Kidneys: A Possible Protective Effect of Selenium

Cisplatin (Cis-diamminedichloroplatinum II, CP) is an important chemotherapeutic agent, useful in the treatment of several cancers, but with several side effects such as nephrotoxicity. The present study investigated the possible protective effect of selenium (Se) against CP-induced oxidative stress in the rat kidneys. Male Wistar albino rats were injected with a single dose of cisplatin (7 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.), alone or in combination. The obtained results showed that CP increased lipid peroxidation (LPO) and decreased reduced glutathione (GSH) concentrations, suggesting the CP-induced oxidative stress, while Se treatment reversed this change to control values. Acute intoxication of rats with CP was followed by statistically significant decreased activity of antioxidant defense enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST). Treatment with Se reversed CP-induced alterations of antioxidant defense enzyme activities and significantly prevented the CP-induced kidney damage

Elektrohemijska degradacija Reactive Black 5 pomocu celicnih elektroda modifikovanih PbO2 i grafitnim ugljen-nitridom

Elektrode modifikovane kompozitom PbO2 sa grafitnim ugljen-nitridom (GCN) upotrebljene su za elekrohemijsku degradaciju tekstilne boje Reactive Black 5. Modifikovana čelična elektroda je korišćena kao anoda, a kao katoda čista čelična elektroda. Ispitan je uticaj strukture PbO2 na elektrokatalitičke karakteristike elektroda. Morfologija materijala je ispitana SEM-om, TEM-om i XRD-om, dok su elektrohemijske osobine ispitane uz pomoć CV i EIS merenja. Efikasnost degradacije je praćena UV-Vis spektrofotometrijom i tečnom hromatografijom. Pri optimalnim parametrima pH, koncentracije pomoćnog elektrolita, jačine struje i koncentracije ispitivane boje, najbolji rezultati su dobijeni sa elektrodom gde je cetiltrimetilamonijum-bromid (CTAB) korišćen kao templat za sintezu PbO2. Pod ovim uslovima je postignuta potpuna degradacija nakon 60 min tretmana, a proizvodi degradacije su određeni korišćenjem HPLC-MS/MS metode.Electrodes modified by PbO2 and graphite carbon nitride (GCN) composite were used for electrochemical degradation of Reactive Black 5 textile dye. A modified steel electrode was used as the anode, while a pure steel electrode was used as the cathode. The influence of PbO2 structre on electrocatalytic characteristics of electrodes was investigated. The morphology of the material was examined by SEM, TEM and XRD, while the electrochemical properties were analyzed using means of CV and EIS measurements. The degradation efficiency was monitored by UV-Vis spectrophotometry and liquid chromatography. At optimal pH parameters, supporting electrolyte concentration, current strength and test dye concentration, the best results were obtained with an electrode where cetyl trimethyl ammonium bromide (CTAB) was used as a template for PbO2 synthesis. Under these conditions, complete degradation was achieved after 60 min of treatment, and degradation products were determined using the HPLC-MS/MS method.Poster: [https://hdl.handle.net/21.15107/rcub_cherry_6059

Elektrohemijska degradacija Reactive Black 5 pomocu celicnih elektroda modifikovanih PbO2 i grafitnim ugljen-nitridom

Elektrode modifikovane kompozitom PbO2 sa grafitnim ugljen-nitridom (GCN) upotrebljene su za elekrohemijsku degradaciju tekstilne boje Reactive Black 5. Modifikovana čelična elektroda je korišćena kao anoda, a kao katoda čista čelična elektroda. Ispitan je uticaj strukture PbO2 na elektrokatalitičke karakteristike elektroda. Morfologija materijala je ispitana SEM-om, TEM-om i XRD-om, dok su elektrohemijske osobine ispitane uz pomoć CV i EIS merenja. Efikasnost degradacije je praćena UV-Vis spektrofotometrijom i tečnom hromatografijom. Pri optimalnim parametrima pH, koncentracije pomoćnog elektrolita, jačine struje i koncentracije ispitivane boje, najbolji rezultati su dobijeni sa elektrodom gde je cetiltrimetilamonijum-bromid (CTAB) korišćen kao templat za sintezu PbO2. Pod ovim uslovima je postignuta potpuna degradacija nakon 60 min tretmana, a proizvodi degradacije su određeni korišćenjem HPLC-MS/MS metode.Electrodes modified by PbO2 and graphite carbon nitride (GCN) composite were used for electrochemical degradation of Reactive Black 5 textile dye. A modified steel electrode was used as the anode, while a pure steel electrode was used as the cathode. The influence of PbO2 structre on electrocatalytic characteristics of electrodes was investigated. The morphology of the material was examined by SEM, TEM and XRD, while the electrochemical properties were analyzed using means of CV and EIS measurements. The degradation efficiency was monitored by UV-Vis spectrophotometry and liquid chromatography. At optimal pH parameters, supporting electrolyte concentration, current strength and test dye concentration, the best results were obtained with an electrode where cetyl trimethyl ammonium bromide (CTAB) was used as a template for PbO2 synthesis. Under these conditions, complete degradation was achieved after 60 min of treatment, and degradation products were determined using the HPLC-MS/MS method.Abstract: [https://hdl.handle.net/21.15107/rcub_cherry_6058

Supplementary data for article : Stanković, D.; Ognjanović, M.; Martin, F.; Šovrc, Ľ.; Mariano, J. F. M. L.; Antic, B. Design of Titanium Nitride- and Wolfram Carbide-Doped RGO/GC Electrodes for Determination of Gallic Acid. Analytical Biochemistry 2017, 539, 104–112. https://doi.org/10.1016/j.ab.2017.10.018

Supplementary material for: [https://doi.org/10.1016/j.ab.2017.10.018]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2560

Supplementary material for the article: Ognjanović, M.; Dojčinović, B.; Fabián, M.; Stanković, D. M.; Mariano, J. F. M. L.; Antić, B. Microwave Assisted Hydrothermal Synthesis of (Fe,Co)3O4 Nanoparticles in the Presence of Surfactants and Effects of Co/Fe Ratio on Microstructure and Magnetism. Ceramics International 2018, 44 (12), 13967–13972. https://doi.org/10.1016/j.ceramint.2018.04.246

Supplementary material for: [https://doi.org/10.1016/j.ceramint.2018.04.246]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2170]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3236

La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells

This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice

Micromolar Levofloxacin Sensor by Incorporating Highly Crystalline Co3O4 into a Carbon Paste Electrode Structure

In this work, we successfully prepared a modified cobalt oxide (Co3O4) carbon paste electrode to detect Levofloxacin (LEV). By synthesizing Co3O4 nanoparticles through the chemical coprecipitation method, the electrochemical properties of the electrode and LEV were thoroughly investigated using CV, SWV, and EIS, while material properties were scrutinized using ICP-OES, TEM, SEM, and XRD. The results showed that the prepared electrode displayed a better electrocatalytic response than the bare carbon paste electrode. After optimizing SWV, the electrode exhibited a wide linear working range from 1 to 85 μM at pH 5 of BRBS as the supporting electrolyte. The selectivity of the proposed method was satisfactory, with good repeatability and reproducibility, strongly suggesting a potential application for determining LEV in real samples, particularly in pharmaceutical formulations. The practicality of the approach was demonstrated through good recoveries, and the morphology of the materials was found to be closely related to other parameters, indicating that the developed method can provide a cost-effective, rapid, selective, and sensitive means for LEV monitoring. Overall, this project has made significant progress towards developing a reliable method for detecting LEV and has opened up new opportunities for future research in this field