Possible immunological basis for recurrent spontaneous abortion: a review

(East African Medical Journal 2001: 78 (11): 586-589

Detection of Hydatidosis in Livestock in Kenya by Indirect Hemagluttination (IHA) Test based on Partially Purified Thermo-stable Antigen

No Abstract.Kenya Veterinarian Vol. 15 1991: pp. 64-6

Immunoglobulin G bound to ovine placenta is eluted by surgical cannulation and acid perfusion in situ

Objective: To elute placental bound immunoglobulin G (IgG) in situ. Design: Laboratory based experimentation.Setting: Biological Sciences Department, The University of Newcastle Australia and the Department of Biochemistry, University of Nairobi, Kenya.Subjects: Twelve pregnant ewes 10 to 15 days before the onset of natural parturition. Results: Placental eluates were rich in IgGl and IgG2. The relative molecular weight of placental IgG was estimated at 158kDa by gel filtration chromatography. Analysis of eluate by SDS PAGE revealed the heavy and light chains of IgG at 57 and 27kDa respectively together giving a relative molecular weight of 168kDa. Conclusion: Placental bound IgG may be crucial in immunology of pregnancy and together with the cognate antigen thereof may be useful as models for the study of maternal-fetal interaction in human pregnancy and in the development of experimental immunotherapy to immunologically compromised pregnancies in humans and livestock.East African Medical Journal Vol. 82(9) 2005: 468-47

Ovine placental eluate immunoglobulins recognise isologous and third party acid-treated trophoblast microvesicle antigens in vitro

Placental microvesicles were prepared from ovine placentae and immunoglobulins eluted with 0.5 M glycine buffer pH 2.5. The ability of eluate immunoglobulins to re-associate with isologous (self) and third party acidified microvesicles was tested by ELISA. Ovine placental immunoglobulins re-associated with isologous and third party acidified microvesicles suggesting that at least 2 types of antigenic epitopes I and II may be expressed on the ovine placentae. Type I antigensmaybe present on placentae of all ovines while type II epitopes may be paternally derived, hence unique to each pregnancy. Analysis by SDS PAGE revealed the heavy and light chains of IgG at 57 and 27 kDa, respectively, together giving a relative molecular weight of 158 kDa. Results suggest that immunoglobulins produced to placental microvesicle antigens may be directed to some but not all antigenic epitopes expressed on the trophoblast, possibly defining a mechanism by which the foetus evades maternal immunological rejection

Combined factor V and factor VIII deficiency in a Thai patient: a case report of genotype and phenotype characteristics

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/71442/1/j.1365-2516.2005.01092.x.pd