Influence of thyroid secretion on the in­duction of leukemia in Dba mice by methylcholanthrene

1) The influence of thyroid secretion upon the induction of leukemia in Dba/2 male mice by methylcholanthrene was investigated. Radiothyroidectomy significantly reduced the incidence of leukemia in these mice. This reduction in incidence did not occur if radiothyroidectomy was performed after the administration of the carcinogen. 2) Data indicated that hypothyroidism following radiothyroidectomy interfered with the initiation rather than the promotion of methylcholanthrene- induced-Ieukemogenesis. 3) No correlation between incidence of leukemia and body weights in the mice was noted.</p

An Eye-Contour Extraction Algorithm from Face Image usingDeformable Template Matching

A variety of studies on face components such as eyes, lips, noses, and teeth have been proceeding in medicine, psychology, biometrics authentication, and other areas. In this paper, we present an algorithm of extracting eye contours from a face image using the deformable template matching method. Our template for an eye contour is composed of three quadratic functions for the perimeter and one circle for the pupil. In our algorithm, a digital color face image is rst converted to a binary image of representing eyes, after the region around eyes is identied on the face image by using hues and values of the color image. Then, parameters in the template are optimized by a local search method with a tabu period and a hill-climbing, so as to t the template to the eye contour in the binary image. The accuracy of our algorithm is evaluated through sample face images of students. In addition, the application of our proposal to eye shape indices is investigated in a face image database "HOIP", where recognizable dierence exists in index distributions between males and females

Urethan(ethyl carbamate)as a multipotential carcinogen in Balb/C,Zb and Db female mice.

1. The objective of this investigation was to test the influence of mammary cancer tissue extract on the induction of various tumors by urethan. Three strains of female mice, Balb/c, Zb and Db, were used in this experiment. 2. It was found that urethan was a multipotential carcinogen in the induction of (a) lung tumor, ovarian hemorrhagic cyst, and hemorrhagic lesions in various tissue in Balb/c mice, (b) lung tumor, hepatoma, leukemia, mammary cancer, Harderian gland tumor and hemorrhagic lesions in various tissues such as spleen, mesenteric lymphnode, liver and ovary in Zb strain, and (c) lung tumor, mediastinal lymphoma, leukemia, hepatoma and hemorrhagic lesions in the liver and ovary in Db mice. 3. Mammary cancer tissue extract seemed to have a promotive effect on the generalization of the mediastinal lymphoma induced by urethan in Db female mice.</p

Participation of DNA polymerases alpha and beta in unscheduled DNA synthesis in mammalian cells.

Repair polymerases participating in unscheduled DNA synthesis in isolated liver nuclei, bleomycin-treated permeable cells and in ultraviolet-irradiated living cells were studied using two specific inhibitors of DNA polymerases, aphidicolin and 2', 3'-dideoxythymidine-5'-triphosphate. Unscheduled, i.e., repair, DNA synthesis in rat liver nuclei, and in bleomycin-treated permeable SR-C3H/He and XC cells was mostly attributed to DNA polymerase beta. Unscheduled DNA synthesis in human liver nuclei, bleomycin-treated permeable HeLa and HEp-2 cells, and in ultraviolet-irradiated HeLa, HEp-2 and XC cells was partially inhibited by the polymerase alpha-specific inhibitor, aphidicolin. The results suggested that both DNA polymerase alpha and beta participated in unscheduled DNA synthesis, though the respective degrees of participation differed depending on cell type and the nature and degree of DNA damage.</p

Differential sensitivity to aphidicolin of replicative DNA synthesis and ultraviolet-induced unscheduled DNA synthesis in vivo in mammalian cells.

In vivo in mammalian cells, ultraviolet-induced unscheduled DNA synthesis was less sensitive to aphidicolin than was replicative DNA synthesis. Replicative DNA synthesis in HeLa, HEp-2, WI-38 VA-13 and CV-1 cells was inhibited more than 97% by aphidicolin at 10 micrograms/ml, whereas aphidicolin inhibition of DNA synthesis in ultraviolet-irradiated cells varied between 30% and 90% depending on cell types and assay conditions. Aphidicolin inhibition of unscheduled DNA synthesis (UDS) in HeLa cells increased gradually with increasing aphidicolin concentration and reached approximately 90% at 100 micrograms/ml aphidicolin. A significant fraction of UDS in ultraviolet-irradiated HEp-2 cells was resistant to aphidicolin even at 300 micrograms/ml. Considered along with related information reported previously, the present results suggest that both aphidicolin-sensitive and insensitive DNA polymerases, DNA polymerase alpha and a non-alpha DNA polymerase (possibly DNA polymerase beta), are involved in in situ UDS in these ultraviolet-irradiated cells. Comparison of staphylococcal nuclease sensitivity between DNAs repaired in the presence and in the absence of aphidicolin in HEp-2 cells suggested that the involvement of DNA polymerase alpha in UDS favored DNA synthesis in the intranucleosomal region.</p

Complete in vitro replication of SV40 DNA and chromatin in saponin-treated permeable cells.

A permeable cell system has been developed by treatment with saponin for studying in vitro replication of DNA and chromatin. DNA replication of simian virus 40 nucleoprotein complexes (SV40 chromatin) in saponin-treated permeable cells was found to be more efficient than that in digitonin-treated permeable cells. Autoradiography of the agarose-gel revealed that [alpha-32P]dCTP was incorporated into SV40 DNA I, II and replicating intermediates. The time course of the incorporation indicated complete replication of SV40 DNA and chromatin with a full number of nucleosomes. The saponin-treated permeable cell system will serve as a useful system for studying in vitro replication of DNA and chromatin in eukaryotic cells.</p

Vonoprazan prevents ulcer recurrence during long-term NSAID therapy: randomised, lansoprazole-controlled non-inferiority and single-blind extension study

Objective To assess the non-inferiority of vonoprazan to lansoprazole for secondary prevention of non-steroidal anti-inflammatory drug (NSAID)-induced peptic ulcer (PU) and the safety of vonoprazan during extended use.Design A phase 3, 24-week, multicenter, randomised, double-blind (DB), active-controlled study, followed by a phase 3, ≥28 week, multicenter, single-blind, parallel-group extension study (EXT) in outpatients (n=642) receiving long-term NSAID therapy who are at risk of PU recurrence. The patients received vonoprazan (10 mg or 20 mg) or lansoprazole 15 mg once daily. For DB, non-inferiority of the proportion of patients with recurrent PU within 24 weeks was analysed by Farrington and Manning test (significance level 2.5%, non-inferiority margin 8.3%; primary endpoint), recurrent PU within 12 weeks, bleeding and time-to-event of PU (secondary endpoint) and treatment-emergent adverse events (TEAEs). For EXT, TEAEs (primary endpoint), recurrent PU and safety (secondary) were assessed up to 104 weeks for patients in the extension study.Results The non-inferiority of vonoprazan 10 mg and 20 mg to lansoprazole 15 mg was verified (percentage difference –2.2%,95% CI –6.2% to 1.8%, p<0.001; –2.1%,95% CI –6.1% to 2.0%, p<0.001, respectively). The proportion of patients with endoscopically confirmed recurrent PU within 24 weeks was 3.3%, 3.4% and 5.5%, for vonoprazan 10 mg, 20 mg and lansoprazole 15 mg, respectively. No significant safety concerns were identified.Conclusion The non-inferiority of vonoprazan (10 and 20 mg) was verified in patients receiving long-term NSAIDs in DB; it was effective and well tolerated in EXT for longer than 1 year, with a safety profile similar to lansoprazole (15 mg)

Abrupt rotation of the rashba spin to the direction perpendicular to the surface

金沢大学理工研究域数物科学系The polarization vector of the Rashba spin, which must be parallel to the two-dimensional (2D) plane in an ideal system, is found to change abruptly and definitely to the direction perpendicular to the surface at the K̄ point of the Brillouin zone of a real hexagonal system, the Tl/Si(111)-(1×1) surface. This finding obtained experimentally by angle-resolved and spin-resolved photoemission measurements is fully confirmed by a first-principles theoretical calculation. We found that the abrupt rotation of the Rashba spin is simply understood by the 2D symmetry of the hexagonal structure. © 2009 The American Physical Society