Diversidade genética para padrões de gliadinas de raças locais de trigo duro no Noroeste do Iran e no Azerbaijão

The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studies.O objetivo deste trabalho foi identificar padrões de bandas de gliadinas e a extenção da variabilidade genética em genótipos de trigo duro do Noroeste do Iran e da República do Azerbaijão. Gliadinas de 46 raças locais e de quatro cultivares foram avaliadas por meio de análises "acid PAGE". Sessenta e seis bandas polimórficas e 81 padrões foram identificados. Foram encontradas 24 bandas de mobilidade e 22 padrões na região da gliadina ω, com 14 bandas polimorfas e 20 padrões para as gliadinas α e γ , e 14 bandas e 19 padrões diferentes para as gliadinas β. A combinação desses padrões gerou 38 e 39 combinações, para os locos Gli-1 e Gli-2, respectivamente. O índice de diversidade genética (H) foi maior para as gliadinas α (0,924), seguidas pelas gliadinas ω e γ (0,899 e 0,878, respectivamente), e pela gliadina β (0,866). Alto polimorfismo (H = 0,875) foi observado em quatro regiões dos padrões de gliadinas obtidos, com diversidade genética maior nas raças locais iranianas do que nas do Azerbaijão. Cada genótipo apresentou padrões de identificação especiais na análise "acid PAGE" de gliadinas, e a análise de agrupamento baseada no coeficiente de similaridade de Jaccard formou seis grupos. A gliadina é de análise simples, repetível e econômica, e pode ser usada em estudos genéticos

Genetic diversity for gliadin patterns of durum wheat landraces in the Northwest of Iran and Azerbaijan

The objective of this study was to identify gliadin band patterns and the extent of genetic diversity in durum wheat genotypes from Northwestern Iran and the Republic of Azerbaijan. Gliadins from 46 landraces and four cultivars were evaluated through acid PAGE analyses. Sixty-six polymorphic bands and 81 patterns were identified. Twenty-four different motility bands and 22 patterns were found in the ω gliadin region with 14 polymorph bands and 20 patterns for α and γ gliadins, and 14 bands and 19 different patterns for β gliadins. The combination of these patterns generated 38 and 39 combinations for Gli-1 and Gli-2 loci, respectively. The genetic diversity index (H) was higher for α gliadins (0.924), followed by ω and γ gliadins (0.899 and 0.878, respectively), and for β gliadin patterns (0.866). Extensive polymorphism (H = 0.875) was observed in four gliadin pattern regions, with higher genetic diversity in the Iranian landraces than in the Azerbaijani ones. Each genotype had special identifying patterns in the gliadin acid PAGE analysis, and cluster analysis based on Jaccard's similarity coefficients formed six groups. Gliadin has a simple, repeatable and economic analysis, and can be used in genetic studie