Screening of UV-irradiated and S-2-aminoethyl-Lcysteine resistant mutants of Bacillus megaterium for improved lysine accumulation

The screening of UV irradiated and aminoethyl-l-cysteine resistant (AECR) mutants of Bacillus megaterium strains for improved lysine accumulation was conducted. The mutants, MR-10 and MR-25 derived form irradiated cultures of B. megaterium SP 76, and MS-3 and MS-5 derived form B.megaterium SP 86 produced higher lysine levels than the parent strains. In contrast, however, lysine accumulation by MV-2 and MV-18 mutants of B. megaterium SP 14, was of low yield. In aminoethyl-lcysteineresistant mutants, R-14 and R-76, derived from B. megaterium SP 14 and B. megaterium SP 76 respectively, lysine accumulation was enhanced

Effect of vitamins and bivalent metals on lysine yield in Bacillus megaterium

The effects of vitamins and bivalent metals on lysine accumulation in Bacillus strains were investigated. Biotin enhanced lysine production in all the Bacillus strains, while folic acid and riboflavin stimulated lysine yields in Bacillus megaterium SP 86 only. All bivalent metals stimulated lysineaccumulation in B. megaterium SP 86 and B. megaterium SP 14, while Co2+ and Zn2+ improved lysine levels in all the strains

Antibiotic and surfactant effects on lysine accumulation by Bacillus megaterium

The effects of antibiotics and surfactants on lysine accumulation in the culture broth of three strains of Bacillus megaterium (B. megaterium SP 86, B. megaterium SP 76 and B. megaterium SP 14) were investigated. Lincomycin, neomycin and tetracycline stimulated lysine increase in B. megaterium SP 76 and B. megaterium SP 14, while erythromycin enhanced lysine yield in all three strains. Palmitic acid improved lysine accumulation in B. megaterium SP 86 and B. megaterium SP 14. However, Tween 80and linoleic acid stimulated lysine increase in B. megaterium SP 76