Anti-Phospholipid Syndrome In Nigeria: Report Of Five Cases

Five cases of secondary anti-phospholipid syndrome (APS) are presented andliterature reviewed. Pregnancy loss was the most common presentation but neurologic manifestations are also seen. IgG ACA was more commonly seen than IgM ACA. Although APS has been infrequently reported in black Africans, an awareness of this condition is needed especially among females with previous recurrent pregnancy losses

Rheumatoid Arthritis Associated with Pulmonary Fibrosis in Nigerians: Two Case Reports

Rheumatoid arthritis may sometimes present with extra-articular involvement, pulmonary involvement is not common. Rheumatoid arthritis has been reported among Nigerians and extra-articular manifestations are rarely seen. One of the patients was misdiagnosed and mismanaged as a patient with pulmonary tuberculosis. The study is to demonstrate that rheumatoid arthritis is not as rare as previously reported in Nigeria and its pulmonary involvement can mimic tuberculosis or other granulomatous lung disorder. Clinical and serological acumen are necessary to distinguish between the two. Two diagnosed patients with rheumatoid arthritis and pulmonary involvement seen at Olabisi Onabanjo University Teaching Hospital (OOUTH), are hereby presented

Connective Tissue Diseases: Challenges of management among Nigerians

Contrary to previous assumptions, Connective Tissue Diseases (CTD) are increasingly being reported among Africans, Nigerians inclusive. The clinical presentations are mostly similar to patterns described in other parts of the world, though with few differences. The management is patterned, as universally done, but drugs such as the biologics are infrequently used because of the prohibitive cost. The challenges of management arise mainly from a poor understanding of the spectrum, presentation and management of CTD as well as the non-affordability of the required drugs. The superstitious belief that these poorly-understood conditions are due to 'spiritual attacks' further compounds the problem of late presentation and sometimes accounts for the high morbidity and mortality. Continuing training of rheumatologists will enhance the understanding and management of rheumatological disease

Shoulder Pain Syndrome Among Nigerians

Objectives: To identify aetiological causes of shoulder pain syndrome among patients attending a rheumatology clinic, and to highlight the therapeutic options.Design: Retrospective study.Setting: A private rheumatology clinic in Lagos, Nigeria from January 2002 to December 2006.Subjects: Forty female adult patients and 26 male adult patients aged 24-79 years. Main outcome measures: Patient reported outcome in terms of pain relief and increased mobility.Results: Sixty six subjects were seen during the study period. Females were more commonly affected and were mostly middle aged. Adhesive capsulitis was the most common condition while septic arthritis and avascular necrosis were least diagnosed 22.7% of the subjects could not, however, be categorised.Conclusion: Shoulder pain syndrome is common among Nigerians. Diagnosis and management are mostly based on the clinical presentations, especially considering the lack of sensitivity of plain radiographs and the high cost of more sensitive MRI. The treatment of these conditions are standard

Time-dependent stability of used engine oil degradation by cultures of Pseudomonas fragi and Achromobacter aerogenes

Pseudomonas fragi and Achromobacter aerogenes isolated from used engine oil polluted soils were grown in minimal salts medium (MSM) supplemented with used engine oil as sole carbon and energysource to evaluate their ability to biodegrade used engine oil. The two organisms utilized 73.3 and 80.0% of the oil with a degradation rate of 0.073 and 0.08 ml/day respectively. The utilization rate of themixed culture did not differ significantly with an 80.0% utilization and 0.08 ml/day degradation rate. However the rate of utilization was reduced significantly after repeated sub culturing of the organismson nutrient agar for six months with percentage utilization dropping to 33.3, 26.7 and 30.0% respectively for A. aerogenes, P. fragi and the mixed culture. This suggests that the presence of hydrocarbons in thegrowth medium is necessary for the stability of hydrocarbon utilization potentials of the isolates

Biological studies on albino rats fed with Sorghum bicolorstarch hydrolyzed with &#8733-amylase from Rhizopus sp.

Partially purified amylase was extracted from the culture medium of Rhizopus sp. grown in potato dextrose broth for 48 h at room temperature by precipitation with 96.9% ethanol. The enzyme was usedto hydrolyze sorghum starch. The hydrolyzed product was afterwards formulated into rat feed, which was fed to albino rats for a period of thirty days. The average daily body weight of the albino rats fed with hydrolyzed formulated feed on the 30th day of the experiment was 131 g while the values recorded for the groups fed with unhydrolyzed and commercial feed were 120 and 97.4 grams respectively. Thehematological analysis revealed that the packed cell volume (PCV), Hemoglobin (Hb), red blood cells (RBC), mean cell hemoglobin concentration (MCHC) of the group fed with hydrolyzed formulated feed of 51.8%, 16.9 g/dl, 8.7 x 105 ƒÊl-1 and 32.7%, respectively, were higher than the experimental animals fed with commercial feed with values of 44.2%, 14.4 g/dl, 7.7 x 10

Determination of the growth rate and volume of lipid produced by Lipomyces species isolated from shear butter leaf (Vitellaria paradoxa)

A Lipomyces strains was isolated from shear butter leaf (Vitellaria paradoxa) by placing the leaf sample in 10 ml of sterile distilled water containing 0.002 g of potassium dihydrogen phosphate and incubatedfor 3 days at 28oC. A drop of this was subsequently streaked nitrogen free medium. For determination of growth rate and volume of lipid produced, 24 h culture of the Lipomyces species isolated was washed into each of the following medium: yeast extract both (YE), nitrogen free broth (NF), maize broth free of salts (MF), maize broth with salts (MB), sorghum broth with salts (SB) and sorghum broth free of salts (SF). These were incubated for 7 days at 28oC on a shaker, and the lipid produced was extracted by using diethyl ether. The Lipomyces species was found to be able to grow and produce lipid more efficiently in yeast extract broth than in other medium used. The organism produced 25 ml of lipid per 8 g of glucose in yeast extract broth

Biodegradation of hydrocarbon compounds in Agbabu natural bitumen

The recovery of an environment polluted by petroleum and allied hydrocarbons through bioremediation is being embraced globally as the best technology of removing hydrocarbon pollutants from environment. Infrared spectral changes and gravimetric analysis from the preliminary biodegradability study carried out on Agbabu Natural Bitumen showed the vulnerability of the bitumen to some bacteria: Pseudomonas putrefaciens, Pseudomonas nigrificans, Bacillus licheniformis, Pseudomonas fragi and Achromobacter aerogenes. This study investigates the ability of P. putrefaciens, P. nigrificans, B. licheniformis, P. fragi and A. aerogenes to degrade the aliphatic and polycyclic aromatic hydrocarbon fractions of Agbabu natural bitumen. Samples of the bitumen were separately inoculated with each of the bacteria for 14 days and the hydrocarbon profiles before and after inoculation were quantified using gas chromatography technique. The total aliphatic hydrocarbon compounds (C11 - C29) in the bitumen degraded by P. putrefaciens and P. nigrificans was slightly higher than that in the undegraded bitumen, while the concentration of compounds (C11 - C29) found in samples of the bitumen degraded by B. licheniformis, P. fragi and A. aerogenes was less than what was contained in the undegraded bitumen. Also the even-odd carbon-ratios of the degraded bitumen were higher than unity while these were less than unity in the undegraded bitumen. The polycyclic aromatic hydrocarbons (PAHs) profile in the bitumen degraded samples also differed from that of undegraded bitumen. A substantial reduction in the concentration of some PAHs was found in the bitumen samples following their degradation by the bacteria strains, typically from 55.98 to 30.79%, thus suggesting the possibility of using the bacteria strains for bioremediation process.Keywords: Agbabu, bitumen, bacteria, biodegradability, hydrocarbonsAfrican Journal of Biotechnology, Vol 13(11), 1257-126