240 research outputs found
The first Japanese word "chishitsu-gaku" for the "geology" was proposed by Genpo Mitsukuri (1799-1863)
The first Japanese word "chishitsu-gaku" for the "geology" was proposed by Genpo Mitsukuri, a scientific contributor in the Tokugawa regime in the 19th century, who was born in 1799 in Tsuyama in Western Honshu, Japan and had worked for scientific activities of the Tokugawa government from 1839 to 1863. His major works for the first making of the "chishitsu-gaku" for the "geology" have been presented in this paper, also showing his contribution to Japanese sciences
Gene expression changes during caste-specific neuronal development in the damp-wood termite Hodotermopsis sjostedti
<p>Abstract</p> <p>Background</p> <p>One of the key characters of social insects is the division of labor, in which different tasks are allocated to various castes. In termites, one of the representative groups of social insects, morphological differences as well as behavioral differences can be recognized among castes. However, very little is known about the neuronal and molecular bases of caste differentiation and caste-specific behavior. In almost all termite species, soldiers play defensive roles in their colonies, and their morphology and behavior are largely different from workers (or pseudergates). Therefore, we predicted that some genes linked to defensive behavior and/or those required for neuronal changes are differentially expressed between workers and soldiers, or during the soldier differentiation, respectively.</p> <p>Results</p> <p>Using the brain and suboesophageal ganglion (SOG) of the damp-wood termite <it>Hodotermopsis sjostedti</it>, we first screened genes specifically expressed in soldiers or during soldier differentiation by the differential display method, followed by quantitative real-time polymerase chain reaction. No distinctive differences in expression patterns were detected between pseudergates and soldiers. In the course of soldier differentiation, however, five genes were found to be up-regulated in brain and/or SOG: 14-3-3epsilon, fibrillin2, beta-tubulin, ciboulot, and a hypothetical protein containing a SAP motif. Some of these genes are thought to be associated with cytoskeletal structure or motor-associated proteins in neuronal tissues.</p> <p>Conclusion</p> <p>The identified five genes could be involved in soldier-specific neuronal modifications, resulting in defensive behaviors in termite soldiers. The temporal expression patterns of these genes were consistent with the neuronal changes during soldier differentiation, suggesting that molecular machineries, in which the identified factors would participate, play important roles in behavioral differentiation of termite soldiers.</p
Structure of the carboxyl-terminal Src kinase, Csk
This research was originally published in Journal of Biological Chemistry. Akira Ogawa, Yoshiharu Takayama, Hiroaki Sakai, Khoon Tee Chong, Satoru Takeuchi, Atsushi Nakagawa, Shigeyuki Nada, Masato Okada and Tomitake Tsukihara. Structure of the carboxyl-terminal Src kinase, Csk. Journal of Biological Chemistry. 2002; 277, 14351-14354. © the American Society for Biochemistry and Molecular Biology
Crystalline Silicate Feature of the Vega-like star HD145263
We have observed the 8-13 m spectrum (R250) of the Vega-like star
candidate HD145263 using Subaru/COMICS. The spectrum of HD145263 shows the
broad trapezoidal silicate feature with the shoulders at 9.3 m and 11.44
m, indicating the presence of crystalline silicate grains. This detection
implies that crystalline silicate may also be commonly present around Vega-like
stars. The 11.44 m feature is slightly shifted to a longer wavelength
compared to the usual 11.2-3 m crystalline forsterite feature detected
toward Herbig Ae/Be stars and T Tauri stars. Although the peak shift due to the
effects of the grain size can not be ruled out, we suggest that Fe-bearing
crystalline olivine explains the observed peak wavelength fairly well.
Fe-bearing silicates are commonly found in meteorites and most interplanetary
dust particles, which originate from planetesimal-like asteroids. According to
studies of meteorites, Fe-bearing silicate must have been formed in asteroidal
planetesimals, supporting the scenario that dust grains around Vega-like stars
are of planetesimal origin, if the observed 11.44 m peak is due to
Fe-bearing silicates.Comment: accepted for Publication in ApJ
Gene up-regulation in response to predator kairomones in the water flea, Daphnia pulex
<p>Abstract</p> <p>Background</p> <p>Numerous cases of predator-induced polyphenisms, in which alternate phenotypes are produced in response to extrinsic stimuli, have been reported in aquatic taxa to date. The genus <it>Daphnia </it>(Branchiopoda, Cladocera) provides a model experimental system for the study of the developmental mechanisms and evolutionary processes associated with predator-induced polyphenisms. In <it>D. pulex</it>, juveniles form neckteeth in response to predatory kairomones released by <it>Chaoborus </it>larvae (Insecta, Diptera).</p> <p>Results</p> <p>Previous studies suggest that the timing of the sensitivity to kairomones in <it>D. pulex </it>can generally be divided into the embryonic and postembryonic developmental periods. We therefore examined which of the genes in the embryonic and first-instar juvenile stages exhibit different expression levels in the presence or absence of predator kairomones. Employing a candidate gene approach and identifying differentially-expressed genes revealed that the morphogenetic factors, <it>Hox3</it>, <it>extradenticle </it>and <it>escargot</it>, were up-regulated by kairomones in the postembryonic stage and may potentially be responsible for defense morph formation. In addition, the juvenile hormone pathway genes, <it>JHAMT </it>and <it>Met</it>, and the insulin signaling pathway genes, <it>InR </it>and <it>IRS-1</it>, were up-regulated in the first-instar stage. It is well known that these hormonal pathways are involved in physiological regulation following morphogenesis in many insect species. During the embryonic stage when morphotypes were determined, one of the novel genes identified by differential display was up-regulated, suggesting that this gene may be related to morphotype determination. Biological functions of the up-regulated genes are discussed in the context of defense morph formation.</p> <p>Conclusions</p> <p>It is suggested that, following the reception of kairomone signals, the identified genes are involved in a series of defensive phenotypic alterations and the production of a defensive phenotype.</p
Synthesis and physical properties of Ca1-xRExFeAs2 with RE = La ~ Gd
Synthesis of a series of layered iron arsenides Ca1-xRExFeAs2 (112) was
attempted by heating at 1000 C under a high-pressure of 2 GPa. The 112 phase
successfully forms with RE = La, Ce, Nd, Sm, Eu and Gd, while Tb, Dy and Ho
substituted and RE free samples does not contain the 112 phase. The Ce, Nd, Sm,
Eu and Gd doped Ca1-xRExFeAs2 are new compounds. All of them exhibit
superconducting transition except for the Ce doped sample. The behaviour of the
critical temperature, with the RE ionic radii have been investigated
Targeted single-cell gene induction by optimizing the dually regulated CRE/loxP system by a newly defined heat-shock promoter and the steroid hormone in Arabidopsis thaliana
Multicellular organisms rely on intercellular communication systems to organize their cellular functions. In studies focusing on intercellular communication, the key experimental techniques include the generation of chimeric tissue using transgenic DNA recombination systems represented by the CRE/loxP system. If an experimental system enables the induction of chimeras at highly targeted cell(s), it will facilitate the reproducibility and precision of experiments. However, multiple technical limitations have made this challenging. The stochastic nature of DNA recombination events, especially, hampers reproducible generation of intended chimeric patterns. Infrared laser-evoked gene operator (IR-LEGO), a microscopic system that irradiates targeted cells using an IR laser, can induce heat shock-mediated expression of transgenes, for example, CRE recombinase gene, in the cells. In this study, we developed a method that induces CRE/loxP recombination in the target cell(s) of plant roots and leaves in a highly specific manner. We combined IR-LEGO, an improved heat-shock-specific promoter, and dexamethasone-dependent regulation of CRE. The optimal IR-laser power and irradiation duration were estimated via exhaustive irradiation trials and subsequent statistical modeling. Under optimized conditions, CRE/loxP recombination was efficiently induced without cellular damage. We also found that the induction efficiency varied among tissue types and cellular sizes. The developed method offers an experimental system to generate a precisely designed chimeric tissue, and thus, will be useful for analyzing intercellular communication at high resolution in roots and leaves
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