Automated Dynamic Resource Provisioning and Monitoring in Virtualized Large-Scale Datacenter

Infrastructure as a Service (IaaS) is a pay-as-you go based cloud provision model which on demand outsources the physical servers, guest virtual machine (VM) instances, storage resources, and networking connections. This article reports the design and development of our proposed innovative symbiotic simulation based system to support the automated management of IaaS-based distributed virtualized data enter. To make the ideas work in practice, we have implemented an Open Stack based open source cloud computing platform. A smart benchmarking application "Cloud Rapid Experimentation and Analysis Tool (aka CBTool)" is utilized to mark the resource allocation potential of our test cloud system. The real-time benchmarking metrics of cloud are fed to a distributed multi-agent based intelligence middleware layer. To optimally control the dynamic operation of prototype data enter, we predefine some custom policies for VM provisioning and application performance profiling within a versatile cloud modeling and simulation toolkit "CloudSim". Both tools for our prototypes' implementation can scale up to thousands of VMs, therefore, our devised mechanism is highly scalable and flexibly be interpolated at large-scale level. Autonomic characteristics of agents aid in streamlining symbiosis among the simulation system and IaaS cloud in a closed feedback control loop. The practical worth and applicability of the multiagent-based technology lies in the fact that this technique is inherently scalable hence can efficiently be implemented within the complex cloud computing environment. To demonstrate the efficacy of our approach, we have deployed an intelligible lightweight representative scenario in the context of monitoring and provisioning virtual machines within the test-bed. Experimental results indicate notable improvement in the resource provision profile of virtualized data enter on incorporating our proposed strategy

Intercellular trafficking and protein delivery by a herpesvirus structural protein

AbstractWe show that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin. VP22 movement was observed both after delivery of DNA by transfection or microinjection and during virus infection. Moreover, we demonstrate that VP22 trafficking occurs via a nonclassical Golgi-independent mechanism. Sensitivity to cytochalasin D treatment suggests that VP22 utilizes a novel trafficking pathway that involves the actin cytoskeleton. In addition, we demonstrate intercellular transport of a VP22 fusion protein after endogenous synthesis or exogenous application, indicating that VP22 may have potential in the field of protein delivery

PHOTOELECTRON-SPECTROSCOPY OF THE TIN DICHALCOGENIDES SNS2-XSEX INTERCALATED WITH COBALTOCENE

Single crystals of the n-type semiconducting tin dichalcogenides SnS2-xSex (x = 0, 0.3, 0.5, 1.3, 1.85, 2), which have a two-dimensional layered structure, have been intercalated with cobaltocene to give the series of compounds SnS2-xSex(CoCp2)0.33, where Cp = η5-C5H5. Photoelectron spectroscopy has been employed to investigate the electronic changes upon intercalation, especially the electron transfer from the guest to the host. X-ray photoelectron spectroscopy (XPS) has revealed mixed oxidation states for both tin [Sn(II), Sn(IV)] and cobalt [Co(I), Co(II), Co(III)]. Of the three cobalt species observed by XPS, two have been unambiguously identified as CoCp2 and [COCp2]+, whereas the third cobalt species has only been tentatively assigned to a Co(η5-C5H5(η4-C 5H5R) complex, in which cobalt is formally in the oxidation state Co(I). Ultraviolet photoelectron spectroscopy (UVPES) shows that the intercalates are either semiconducting (x = 0, 0.3, 0.5, 1.3) or metallic (x = 1.85, 2), whereas all the hosts are n-type semiconductors. An impurity-band model is presented as a possible qualitative explanation for this transition through the series. © 1990 American Chemical Society

SYNTHESIS OF PERMETHYLINDENYL COMPLEXES OF THE EARLY TRANSITION-METALS - CRYSTAL-STRUCTURES OF TI(ETA(5)-C(9)ME(7))CL-3 AND ZR(ETA(5)-C(9)ME(7))(2)CL-2

Addition of 1-(trimethylsilyl)heptamethylindene (C9Me7SiMe3) to a suspension of the anhydrous halides MCl4 (M = Ti, Zr, and Hf) in THF gives the half-sandwich complexes M(η5-C9Me7)Cl3 (M = Ti, Zr, and Hf) in good yield. Ti(η5-C9Me7)2Cl3 crystallizes in the centrosymmetric orthorhombic space group Pbca with a = 16.875(4) Å, b = 14.706(3) Å, c = 13.781(3) Å, V = 3420 Å3, Z = 8, R = 0.047, and Rw = 0.049. The Ti-C distances range from 2.352(4) to 2.400(4) Å with a mean value of 2.370(5) Å and are consistent with symmetrical bonding of the indenyl ligand to the metal center. The Ti-(η5-ring centroid) distance is 2.036(4) Å. Addition of 2 equiv of lithium heptamethylindenide (Li+C9Me7-) to the anhydrous halides MCl4 (M = Zr and Hf) in THF gives the metallocene derivatives M(η5-C9Me7)2Cl2 (M = Zr and Hf) in good yield. Zr(η5-C9Me7)2Cl2 crystallizes in the centrosymmetric monoclinic space group P21/n with a = 14.951(12) Å, b = 13.523(6) Å, c = 13.913(11) Å, β = 96.46(3)°, V = 2795.5 Å3, R = 0.041, and Rw = 0.048. The Zr-C distances range from 2.511(4) to 2.612(4) Å, with a mean value of 2.563(4) Å, and are consistent with symmetrical η5-bonding of both indenyl ligands to the metal center. The Zr-(η5-ring centroid) distances are 2.259(4) and 2.255(4) Å with a centroid-Zr-centroid angle of 139.0(4)°. The indenyl ligands are oriented away from the chloride ligands which causes a significant distortion from planarity by folding at the bridgehead carbon atoms. © 1994 American Chemical Society