Hormonal panel of various breeds of cattle under the conditions of temperature stress

The article gives the data on the content of a number of steroid and thyroid hormones in the population of Tagil, Suksun and Holstein cattle under the conditions of increasing environmental temperature resulting in moderate stress reaction, as well as on the changes in endocrine system after temperature stress. Changes in function of vascular glands were found showing high resistance of Tagil cattle, moderate resistance of Suksun cattle and low resistance of Holstein cattle to temperature stress. The most significant changes were noted in thyroid panel of Tagil cattle that was shown by increasing synthesis of iodothyronine while environmental temperature changed from high to normal values (during post-stress period). The breeds with lower resistance had the signs of deterioration of adaption mechanism expressed in stronger unbalance reaction of hormonal axis

Complex compounds of transition metals with hydroxyaromatic carboxylic acids as precursors for the synthesis of nanosized metal oxides

Eight complex compounds of MnII, FeII, CoII, NiII and CuII with 2,4,6-trihydroxy benzoic acid and 3,4,5-trihydroxybenzoic acid were synthesized in a crystalline form. Nanosized metal oxide particles obtained by thermal decomposition of the complexes were studied by X-ray powder dif raction analysis and electron microscopy. © 2020, Springer Science+Business Media LLC

Comparative characterization of two tests for measurement of hepatitis B virus DNA in the blood serum and plasma, based on the use of two different detection methods

Two tests for measurements of hepatitis B virus DNA in human serum and plasma, VERSANT HBV 3.0 (based on the use of branched DNA chains) and Biotitre-B (a realtime PCR variant), were compared. Serum specimens from patients (n=56) with documented viral hepatitis were tested. For specimens with DNA values in the linear range in both tests the correlations were evaluated by Pearson's method. The sensitivity of the two tests, and reproducibility of the results of HBV DNA measurements were evaluated using a panel of recombinant virus DNA dilutions with a step of 1 lg HBV DNA copies per ml (from 7 to 3 lg). Three measurements for each concentration of the reference sample were carried out in each test. Clinical specificity of the two tests was evaluated by the analysis of HBV-negative serum samples, collected from donors not reactive by HBsAg and anti-HBc (n=60). Of 56 samples with documented infection, the results of testing by VERSANT HBV 3.0 and Biotitre-B did not agree in 4 (7.1%) samples. Pearson's correlation coefficient of for results obtained in VERSANT HBV 3.0 and Biotitre-B in linear range for both tests was 0.712. Evaluation of reproducibility of the tests using a panel of recombinant HBV DNA showed higher reproducibility of VERSANT HBV 3.0 test with coefficient of variations from 0.79 to 2.79% vs. 2.39-10.69% for Biotitre-B reference test. All 60 serum samples from donors areactive by HBsAg and anti-HBc were negative by HBV DNA when tested by VERSANT HBV 3.0 and Biotitre-B. Hence, clinical specificity of both tests was 100%. The results indicate high specificity of both tests and good agreement of their results, the reproducibility of VERSANT HBV 3.0 test being higher. © 2008 Springer Science+Business Media, Inc

Hormonal panel of various breeds of cattle under the conditions of temperature stress

The article gives the data on the content of a number of steroid and thyroid hormones in the population of Tagil, Suksun and Holstein cattle under the conditions of increasing environmental temperature resulting in moderate stress reaction, as well as on the changes in endocrine system after temperature stress. Changes in function of vascular glands were found showing high resistance of Tagil cattle, moderate resistance of Suksun cattle and low resistance of Holstein cattle to temperature stress. The most significant changes were noted in thyroid panel of Tagil cattle that was shown by increasing synthesis of iodothyronine while environmental temperature changed from high to normal values (during post-stress period). The breeds with lower resistance had the signs of deterioration of adaption mechanism expressed in stronger unbalance reaction of hormonal axis

Comparative characterization of two tests for measurement of hepatitis B virus DNA in the blood serum and plasma, based on the use of two different detection methods

Two tests for measurements of hepatitis B virus DNA in human serum and plasma, VERSANT HBV 3.0 (based on the use of branched DNA chains) and Biotitre-B (a realtime PCR variant), were compared. Serum specimens from patients (n=56) with documented viral hepatitis were tested. For specimens with DNA values in the linear range in both tests the correlations were evaluated by Pearson's method. The sensitivity of the two tests, and reproducibility of the results of HBV DNA measurements were evaluated using a panel of recombinant virus DNA dilutions with a step of 1 lg HBV DNA copies per ml (from 7 to 3 lg). Three measurements for each concentration of the reference sample were carried out in each test. Clinical specificity of the two tests was evaluated by the analysis of HBV-negative serum samples, collected from donors not reactive by HBsAg and anti-HBc (n=60). Of 56 samples with documented infection, the results of testing by VERSANT HBV 3.0 and Biotitre-B did not agree in 4 (7.1%) samples. Pearson's correlation coefficient of for results obtained in VERSANT HBV 3.0 and Biotitre-B in linear range for both tests was 0.712. Evaluation of reproducibility of the tests using a panel of recombinant HBV DNA showed higher reproducibility of VERSANT HBV 3.0 test with coefficient of variations from 0.79 to 2.79% vs. 2.39-10.69% for Biotitre-B reference test. All 60 serum samples from donors areactive by HBsAg and anti-HBc were negative by HBV DNA when tested by VERSANT HBV 3.0 and Biotitre-B. Hence, clinical specificity of both tests was 100%. The results indicate high specificity of both tests and good agreement of their results, the reproducibility of VERSANT HBV 3.0 test being higher. © 2008 Springer Science+Business Media, Inc

Physicochemical Characteristics and Catalytic Activity of Composite Materials Based on Mixed Oxides MTiO3/TiO2 (М = Co, Ni) in 1,2-Dihydroxybenzene Photodegradation Reactions in Aqueous Solutions

Abstract: Mixed cobalt and nickel oxides MTiO3/TiO2 (M = Co2+, Ni2+) are synthesized. Organometallic complexes of titanium tetrabutylate Ti(OBu)4 with 1,2-dihydroxybenzene and nitrate hydrates of corresponding metals (M) synthesized under conditions of the modified sol-gel process are used as precursors. The synthesized samples are characterized by field-emission scanning electron microscopy, energy-dispersive X-ray analysis, diffuse reflectance spectroscopy, dispersive X-ray radiation analysis, X-ray diffraction, and low-temperature nitrogen adsorption. The photocatalytic activity of the samples is tested in the ultraviolet spectral range (200 nm < λ < 400 nm) in the photodegradation of 1,2-dihydroxybenzene (catechol) in aqueous solutions. © 2020, Pleiades Publishing, Ltd

Physicochemical properties of solutions of bis(2,3,5,6-tetraoxo-4- nitropyridinate) dianions. Crystal structures of sodium 2,3,5,6-tetraoxo-4- nitropyridinate trihydrate and sodium ammonium bis(2,3,5,6-tetraoxo-4- nitropyridinate) monohydrate

Ammonium 2,3,5,6-tetraoxo-4-nitropyridinate was shown to exist as stable dimers and act as a weak electrolyte in both a crystalline state and aqueous solutions. In a strongly alkaline medium, ammonium cations were successively replaced by sodium cations. The molecular and crystal structures of sodium 2,3,5,6-tetraoxo-4-nitropyridinate trihydrate and sodium ammonium bis(2,3,5,6-tetraoxo-4-nitropyridinate) monohydrate were established, and their spectroscopic characteristics were determined. © 2004 MAIK "Nauka/Interperiodica"

Physicochemical properties of solutions of bis(2,3,5,6-tetraoxo-4- nitropyridinate) dianions. Crystal structures of sodium 2,3,5,6-tetraoxo-4- nitropyridinate trihydrate and sodium ammonium bis(2,3,5,6-tetraoxo-4- nitropyridinate) monohydrate

Ammonium 2,3,5,6-tetraoxo-4-nitropyridinate was shown to exist as stable dimers and act as a weak electrolyte in both a crystalline state and aqueous solutions. In a strongly alkaline medium, ammonium cations were successively replaced by sodium cations. The molecular and crystal structures of sodium 2,3,5,6-tetraoxo-4-nitropyridinate trihydrate and sodium ammonium bis(2,3,5,6-tetraoxo-4-nitropyridinate) monohydrate were established, and their spectroscopic characteristics were determined. © 2004 MAIK "Nauka/Interperiodica"