11 research outputs found
Π£ΡΠΈΠ»Π΅Π½ΠΈΠ΅ ΡΠΈΠ³Π½Π°Π»Π° SPR Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ° Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π·ΠΎΠ»ΠΎΡΡΡ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ Π°Π½Π°Π»ΠΈΠ·Π° Π±Π΅ΡΠ°-2-ΠΌΠΈΠΊΡΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½Π° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
The highly sensitive method of surface plasmon resonance (SPR) detection of low concentrations of target proteins based on the biosensor signal enhancement by using gold nanoparticles (similar to βsandwichβ assay type) is described. The commercial protein preparations of beta-2-microglobulin (B2M) as a model biomarker and polyclonal (Pab) and monoclonal antibodies (Mab) to B2M were used. It has been shown that this universal and reproducible method can be applied for SPR analysis of other protein biomarkers by analogy with the biomarker protein B2M. The present work is also focused on the experimental protocol description. The protocols of gold nanoparticles (GNP) synthesis, obtaining the conjugates of Pab/GNP and measuring their concentration, the protocol of Mab covalent immobilization on the optical chip CM5 of a biosensor and also SPR registration of molecular interactions Mab-biomarker and in the βsandwichβ assay type Mab-biomarker-Pab or Mab-biomarker-Pab/GNP are considered in detail.ΠΠΏΠΈΡΠ°Π½ Π²ΡΡΠΎΠΊΠΎΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄ Π΄Π΅ΡΠ΅ΠΊΡΠΈΠΈ Π½ΠΈΠ·ΠΊΠΈΡ
ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΉ ΡΠ΅Π»Π΅Π²ΡΡ
Π±Π΅Π»ΠΊΠΎΠ² Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΡΠΈΠ»Π΅Π½ΠΈΡ ΡΠΈΠ³Π½Π°Π»Π° ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ° Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΠΎΠ³ΠΎ ΠΏΠ»Π°Π·ΠΌΠΎΠ½Π½ΠΎΠ³ΠΎ ΡΠ΅Π·ΠΎΠ½Π°Π½ΡΠ° (SPR) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π·ΠΎΠ»ΠΎΡΡΡ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ (ΠΏΠΎ ΡΠΈΠΏΡ βΡΠ°Π½Π΄Π²ΠΈΡβ). Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΌΠΎΠ΄Π΅Π»ΠΈ Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅ΡΠ° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ ΠΊΠΎΠΌΠΌΠ΅ΡΡΠ΅ΡΠΊΠΈΠΉ Π±Π΅Π»ΠΊΠΎΠ²ΡΠΉ ΠΏΡΠ΅ΠΏΠ°ΡΠ°Ρ Π±Π΅ΡΠ°-2-ΠΌΠΈΠΊΡΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½Π° (B2M), Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
(Pab) ΠΈ ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π» (Mab) ΠΊ B2M. ΠΠ°Π½Π½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄ Π°Π½Π°Π»ΠΈΠ·Π° Π²ΡΠ»Π΅Π΄ΡΡΠ²ΠΈΠ΅ Π΅Π³ΠΎ ΡΠ½ΠΈΠ²Π΅ΡΡΠ°Π»ΡΠ½ΠΎΡΡΠΈ ΠΈ Π²ΠΎΡΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΠΌΠΎΡΡΠΈ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΏΡΠΈΠΌΠ΅Π½ΡΠ½ Π΄Π»Ρ Π°Π½Π°Π»ΠΈΠ·Π° Π»ΡΠ±ΡΡ
Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ². ΠΠΎΠ΄ΡΠΎΠ±Π½ΠΎ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΠ΅ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Ρ ΡΠΈΠ½ΡΠ΅Π·Π° Π·ΠΎΠ»ΠΎΡΡΡ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ (GNP), ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΈΡ
ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΎΠ² Ρ ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΠΌΠΈ Π°Π½ΡΠΈΡΠ΅Π»Π°ΠΌΠΈ (Pab/GNP), ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ ΠΈ ΠΊΠΎΠ½ΡΡΠ³Π°ΡΠΎΠ², ΠΊΠΎΠ²Π°Π»Π΅Π½ΡΠ½ΠΎΠΉ ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ Mab Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠΏΠ° Π‘Π5 Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ°, ΡΠ΅Π³ΠΈΡΡΡΠ°ΡΠΈΠΈ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Mab-Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅Ρ ΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠΈΠΏΠ° βΡΠ°Π½Π΄Π²ΠΈΡβ Mab-Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅Ρ-Pab ΠΈ Mab-Π±ΠΈΠΎΠΌΠ°ΡΠΊΠ΅Ρ-Pab/GNP
Additive N-Step Markov Chains as Prototype Model of Symbolic Stochastic Dynamical Systems with Long-Range Correlations
A theory of symbolic dynamic systems with long-range correlations based on
the consideration of the binary N-step Markov chains developed earlier in Phys.
Rev. Lett. 90, 110601 (2003) is generalized to the biased case (non equal
numbers of zeros and unities in the chain). In the model, the conditional
probability that the i-th symbol in the chain equals zero (or unity) is a
linear function of the number of unities (zeros) among the preceding N symbols.
The correlation and distribution functions as well as the variance of number of
symbols in the words of arbitrary length L are obtained analytically and
verified by numerical simulations. A self-similarity of the studied stochastic
process is revealed and the similarity group transformation of the chain
parameters is presented. The diffusion Fokker-Planck equation governing the
distribution function of the L-words is explored. If the persistent
correlations are not extremely strong, the distribution function is shown to be
the Gaussian with the variance being nonlinearly dependent on L. An equation
connecting the memory and correlation function of the additive Markov chain is
presented. This equation allows reconstructing a memory function using a
correlation function of the system. Effectiveness and robustness of the
proposed method is demonstrated by simple model examples. Memory functions of
concrete coarse-grained literary texts are found and their universal power-law
behavior at long distances is revealed.Comment: 19 pages, 8 figure
ΠΡΡΠΎΠΊΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ° Π½ΠΈΠ·ΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ Π½Π° Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Ρ CYP51 Candida krusei
The opportunistic fungus Candida krusei is the causative agent of nosocomial infections characterized by high mortality and development of resistance to drugs of the azole class. Therefore, develjoment of non-azole antifungal agents against resistant fungal strains is extremly important. Lanosterol 14-alpha demethylase (CYP51) is a well-known antifungal target. The optical SPR biosensor is a universal tool for screening studies in search of new drug prototypes. This paper presents the methodological aspects of high-hroughput SPR based screening of a library of low molecular weight compounds of natural origin for their interaction with C. krusei CYP51. It has been shown that when performing high-throughput screening, a researcher should pay special attention to the degree of a sensorgram curvature in the association phase. The described approaches to the analysis of high throughput screening data can be useful for researchers working with SPR biosensors from various manufacturers.Π£ΡΠ»ΠΎΠ²Π½ΠΎ-ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΠΉ Π³ΡΠΈΠ± Candida krusei (C. krusei) ΡΠ²Π»ΡΠ΅ΡΡΡ Π²ΠΎΠ·Π±ΡΠ΄ΠΈΡΠ΅Π»Π΅ΠΌ Π½ΠΎΠ·ΠΎΠΊΠΎΠΌΠΈΠ°Π»ΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ, Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΠΈΡ
ΡΡ Π²ΡΡΠΎΠΊΠΎΠΉ Π»Π΅ΡΠ°Π»ΡΠ½ΠΎΡΡΡΡ. Π ΠΏΠΎΡΠ»Π΅Π΄Π½Π΅Π΅ Π²ΡΠ΅ΠΌΡ Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΡ ΠΊ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ Π΄ΠΎΠ»ΠΈ ΡΡΠ°ΠΌΠΌΠΎΠ² Π΄ΡΠΎΠΆΠΆΠ΅Π²ΡΡ
Π³ΡΠΈΠ±ΠΎΠ², ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΡΡ
ΠΊ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°ΠΌ ΠΊΠ»Π°ΡΡΠ° Π°Π·ΠΎΠ»ΠΎΠ². ΠΠΎΡΡΠΎΠΌΡ ΠΏΠΎΠΈΡΠΊ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ
ΠΏΡΠΎΡΠΈΠ²ΠΎΠ³ΡΠΈΠ±ΠΊΠΎΠ²ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ Π½Π΅Π°Π·ΠΎΠ»ΡΠ½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΠΌ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΡΠΈ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ΅ Π½ΠΎΠ²ΡΡ
ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΡΠ΅Π΄ΡΡΠ² Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΡΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² Π³ΡΠΈΠ±ΠΎΠ². ΠΠ°Π½ΠΎΡΡΠ΅ΡΠΎΠ» 14-Π°Π»ΡΡΠ° Π΄Π΅ΠΌΠ΅ΡΠΈΠ»Π°Π·Π° (CYP51) ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΈΡΠΎΠΊΠΎ ΠΈΠ·Π²Π΅ΡΡΠ½ΠΎΠΉ ΠΌΠΈΡΠ΅Π½ΡΡ Π΄Π»Ρ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ³ΡΠΈΠ±ΠΊΠΎΠ²ΡΡ
ΡΡΠ΅Π΄ΡΡΠ². ΠΠΏΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ ΡΠΎΠ±ΠΎΠΉ ΡΠ½ΠΈΠ²Π΅ΡΡΠ°Π»ΡΠ½ΡΠΉ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½Ρ Π΄Π»Ρ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ΠΎΠ²ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ, ΡΠ΅Π»ΡΡ ΠΊΠΎΡΠΎΡΡΡ
ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΠΎΠΈΡΠΊ ΠΏΡΠΎΡΠΎΡΠΈΠΏΠΎΠ² Π½ΠΎΠ²ΡΡ
Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ. Π Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π°ΡΠΏΠ΅ΠΊΡΡ Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° Π±ΠΈΠ±Π»ΠΈΠΎΡΠ΅ΠΊΠΈ Π½ΠΈΠ·ΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΠΏΡΠΈΡΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ, Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ Π½Π° ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠ΅ ΠΈΡ
Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Ρ CYP51 C. krusei Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ°. ΠΠ°ΠΊ ΠΏΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ, ΠΏΡΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΈ Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΎΡΠΎΠ±ΠΎΠ΅ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΡΠ»Π΅Π΄ΡΠ΅Ρ ΠΎΠ±ΡΠ°ΡΠ°ΡΡ Π½Π° ΡΡΠ΅ΠΏΠ΅Π½Ρ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π½Π°ΠΊΠ»ΠΎΠ½Π° ΡΠ΅Π½ΡΠΎΠ³ΡΠ°ΠΌΠΌΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Π² ΡΠ°Π·Π΅ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ. ΠΠΏΠΈΡΠ°Π½Π½ΡΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ ΠΊ Π°Π½Π°Π»ΠΈΠ·Ρ Π΄Π°Π½Π½ΡΡ
Π²ΡΡΠΎΠΊΠΎΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΏΠΎΠ»Π΅Π·Π½Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠΌ, ΡΠ°Π±ΠΎΡΠ°ΡΡΠΈΠΌ Ρ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ°ΠΌΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΌΠΎΠ΄Π΅Π»Π΅ΠΉ
ΠΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΠΏΡΠΎΠ±ΠΎΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ Π»ΠΈΠ·Π°ΡΠΎΠ² Π΄Π»Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΡ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΠΏΠ°ΡΡΠ½Π΅ΡΠΎΠ² ΡΠ΅Π»Π΅Π²ΡΡ Π±Π΅Π»ΠΊΠΎΠ², ΠΊΠΎΠ΄ΠΈΡΡΠ΅ΠΌΡΡ Π³Π΅Π½Π°ΠΌΠΈ 18-ΠΎΠΉ Ρ ΡΠΎΠΌΠΎΡΠΎΠΌΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
The aim of this work was to test modifications of the standard protocol for the sample preparation of cell/tissue lysate before performing the affinity isolation of lysate protein partners for the target protein (bait protein) which is covalently immobilized on an inert sorbent (e.g. BrCN-, SH-Sepharose 4B) or a carrier (e.g. paramagnetic nanoparticles). The series of our previous works on applying the approach to direct molecular fishing procedure with combination of affinity chromatography and LC-MS/MS analysis using a number of proteins, encoded by the genes of human chromosome 18, have shown that there are at least two problems affecting the specificity and the effectiveness of this procedure. These include: (i) redundancy of the background proteins in the eluates from an affinity sorbent (carrier) due to isolation of multiprotein complexes βlabeledβ with a direct protein partner which binds with a bait protein immobilized on the sorbent; (ii) low enrichment of the eluates with appropriate protein partners due to the fact that some direct protein partners in the lysate exist in stable βwild typeβ complexes with the bait protein itself. This means that latter group of protein partners will not be sufficiently isolated from lysate. Therefore, in order to increase the specificity and efficiency of affinity isolation of protein partners for the bait protein, we modified the standard protocol of lysate preparation and the preliminary step on dissociation of lysate protein complexes was added. Several model experiments for the choice of regeneration solution, assessment of their efficiency in the dissociation of lysate protein complexes as well as the stability and binding capacity of proteins were performed under the control of surface plasmon resonance (SPR) biosensor Biacore 3000 using HepG2 cell lysate. It was shown that acid treatment and incubation of the cell lysate for one min on ice (final lysate dilution 20 times) and subsequent neutralization (pH shift from 2.0 to 7.4) resulted in maximal dissociation of the lysate protein complexes without significant negative effects on the protein-protein interactions tested.Π¦Π΅Π»ΡΡ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»ΠΎ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ΅ ΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Π° ΠΏΡΠΎΠ±ΠΎΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ/ ΡΠΊΠ°Π½Π΅Π²ΠΎΠ³ΠΎ Π»ΠΈΠ·Π°ΡΠ° ΠΏΠ΅ΡΠ΅Π΄ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΏΡΠΎΡΠ΅Π΄ΡΡΡ Π°ΡΡΠΈΠ½Π½ΠΎΠ³ΠΎ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΡ ΠΈΠ· Π½Π΅Π³ΠΎ Π±Π΅Π»ΠΊΠΎΠ²-ΠΏΠ°ΡΡΠ½Π΅ΡΠΎΠ² Π΄Π»Ρ ΡΠ΅Π»Π΅Π²ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° (Π±Π΅Π»ΠΊΠ°-Π½Π°ΠΆΠΈΠ²ΠΊΠΈ), ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π½Π° ΠΈΠ½Π΅ΡΡΠ½ΠΎΠΌ ΡΠΎΡΠ±Π΅Π½ΡΠ΅ ΠΈΠ»ΠΈ ΠΏΠ°ΡΠ°ΠΌΠ°Π³Π½ΠΈΡΠ½ΡΡ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡΠ°Ρ
. Π¦ΠΈΠΊΠ» Π½Π°ΡΠΈΡ
ΠΏΡΠ΅Π΄ΡΠ΄ΡΡΠΈΡ
ΡΠ°Π±ΠΎΡ, ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π½ΡΡ
ΠΏΡΡΠΌΠΎΠΌΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠΌΡ ΡΠΈΡΠΈΠ½Π³Ρ Ρ ΡΠΎΠΏΡΡΠΆΠ΅Π½ΠΈΠ΅ΠΌ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΈ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΏΠ°ΡΠ°ΠΌΠ°Π³Π½ΠΈΡΠ½ΡΡ
Π½Π°Π½ΠΎΡΠ°ΡΡΠΈΡ c ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΡΠ΄Π° Π±Π΅Π»ΠΊΠΎΠ² 18-ΠΎΠΉ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΈ ΡΠ°ΠΊΠΆΠ΅ Π΄ΡΡΠ³ΠΈΡ
Π±Π΅Π»ΠΊΠΎΠ² ΠΏΠΎΠΊΠ°Π·Π°Π», ΡΡΠΎ ΡΡΡΠ΅ΡΡΠ²ΡΡΡ, ΠΏΠΎ ΠΊΡΠ°ΠΉΠ½Π΅ ΠΌΠ΅ΡΠ΅, Π΄Π²Π΅ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ, Π²Π»ΠΈΡΡΡΠΈΠ΅ Π½Π° ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΡ ΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π΄Π°Π½Π½ΠΎΠΉ ΠΏΡΠΎΡΠ΅Π΄ΡΡΡ: (i) ΠΈΠ·Π±ΡΡΠΎΡΠ½ΠΎΡΡΡ ΡΠΎΠ½ΠΎΠ²ΡΡ
Π±Π΅Π»ΠΊΠΎΠ² Π² ΡΠ»ΡΠ°ΡΠ°Ρ
Ρ Π°ΡΡΠΈΠ½Π½ΠΎΠ³ΠΎ ΡΠΎΡΠ±Π΅Π½ΡΠ°, ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½Π°Ρ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΌΡΠ»ΡΡΠΈΠ±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ², ΠΌΠ΅ΡΠ΅Π½ΡΡ
ΠΏΡΡΠΌΡΠΌ ΠΏΠ°ΡΡΠ½Π΅ΡΠΎΠΌ, ΠΊΠΎΡΠΎΡΡΠΉ ΡΠ²ΡΠ·ΡΠ²Π°Π΅ΡΡΡ Ρ ΡΠ΅Π»Π΅Π²ΡΠΌ Π±Π΅Π»ΠΊΠΎΠΌ Π½Π° ΡΠΎΡΠ±Π΅Π½ΡΠ΅; (ii) Π½ΠΈΠ·ΠΊΠ°Ρ ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½Π½ΠΎΡΡΡ ΡΠ»ΡΠ°ΡΠΎΠ² Π±Π΅Π»ΠΊΠ°ΠΌΠΈ-ΠΏΠ°ΡΡΠ½Π΅ΡΠ°ΠΌΠΈ ΡΠ΅Π»Π΅Π²ΠΎΠΉ Π³ΡΡΠΏΠΏΡ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½Π°Ρ ΡΠ΅ΠΌ, ΡΡΠΎ ΡΠ° ΠΈΠ»ΠΈ ΠΈΠ½Π°Ρ ΡΠ°ΡΡΡ ΠΏΡΡΠΌΡΡ
Π±Π΅Π»ΠΊΠΎΠ²-ΠΏΠ°ΡΡΠ½Π΅ΡΠΎΠ² Π² Π»ΠΈΠ·Π°ΡΠ΅ Π½Π°Ρ
ΠΎΠ΄ΠΈΡΡΡ Π² ΡΠΎΡΡΠ°Π²Π΅ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΡΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² Β«Π΄ΠΈΠΊΠΎΠ³ΠΎ ΡΠΈΠΏΠ°Β» Ρ ΡΠ°ΠΌΠΈΠΌ Π±Π΅Π»ΠΊΠΎΠΌ-Π½Π°ΠΆΠΈΠ²ΠΊΠΎΠΉ ΠΈ Π½Π΅ Π±ΡΠ΄Π΅Ρ Π² Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π²ΡΠ΄Π΅Π»Π΅Π½Π° ΠΈΠ· Π»ΠΈΠ·Π°ΡΠ°. ΠΠΎΡΡΠΎΠΌΡ Π΄Π»Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΡΡΠΈ ΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π°ΡΡΠΈΠ½Π½ΠΎΠ³ΠΎ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΡ Π±Π΅Π»ΠΊΠΎΠ²-ΠΏΠ°ΡΡΠ½Π΅ΡΠΎΠ² ΡΠ΅Π»Π΅Π²ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° Π½Π°ΠΌΠΈ ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Π° ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠΉ ΠΏΡΠΎΠ±ΠΎΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ, Π·Π°ΠΊΠ»ΡΡΠ°ΡΡΠ°ΡΡΡ Π² ΠΏΡΠ΅Π΄Π²Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ Π΄ΠΈΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² Π»ΠΈΠ·Π°ΡΠ°. ΠΠΎΠ΄Π΅Π»ΡΠ½ΡΠ΅ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΡ ΠΏΠΎ Π²ΡΠ±ΠΎΡΡ ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΡΠ°ΡΡΠ²ΠΎΡΠ°, ΠΎΡΠ΅Π½ΠΊΠ΅ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΡΡΠΈ ΠΈ ΡΠ²ΡΠ·ΡΠ²Π°ΡΡΠ΅ΠΉ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ Π±Π΅Π»ΠΊΠΎΠ² ΠΏΡΠΈ Π΅Π³ΠΎ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΡΠ΅Π½ΠΊΠ° ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π΄ΠΈΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² Π² Π»ΠΈΠ·Π°ΡΠ΅ Π±ΡΠ»ΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½Ρ ΠΏΠΎΠ΄ ΠΊΠΎΠ½ΡΡΠΎΠ»Π΅ΠΌ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ° Biacore 3000 (Β«GE HealthcareΒ», Π‘Π¨Π) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π»ΠΈΠ·Π°ΡΠ° ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΡ Π³Π΅ΠΏΠ°ΡΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° (HepG2) ΠΈ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π±Π΅Π»ΠΊΠΎΠ², ΠΊΠΎΠ΄ΠΈΡΡΠ΅ΠΌΡΡ
Π³Π΅Π½Π°ΠΌΠΈ 18-ΠΎΠΉ Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΊΠΈΡΠ»ΠΎΡΠ½Π°Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΡΠ°Π·Π±Π°Π²Π»Π΅Π½Π½ΠΎΠ³ΠΎ Π² 20 ΡΠ°Π· Π»ΠΈΠ·Π°ΡΠ° Ρ ΠΊΡΠ°ΡΠΊΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ ΡΠΊΡΠΏΠΎΠ·ΠΈΡΠΈΠ΅ΠΉ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 1 ΠΌΠΈΠ½ Π½Π° Π»ΡΠ΄Ρ ΠΈ Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅ΠΉ Π½Π΅ΠΉΡΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ (Ρ ΡΠ 2.0 Π΄ΠΎ ΡΠ 7.4) ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»Π° ΠΊ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠΉ Π΄ΠΈΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² Π»ΠΈΠ·Π°ΡΠ°, Π½Π΅ ΠΎΠΊΠ°Π·ΡΠ²Π°Ρ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π²Π»ΠΈΡΠ½ΠΈΡ Π½Π° ΡΠ΅ΡΡΠΈΡΡΠ΅ΠΌΡΠ΅ Π±Π΅Π»ΠΎΠΊ- Π±Π΅Π»ΠΊΠΎΠ²ΡΠ΅ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ
ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ SPR Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ° ΠΏΡΠΈ ΠΏΠΎΠΈΡΠΊΠ΅ ΠΏΡΠΎΡΠΎΡΠΈΠΏΠΎΠ² Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ ΡΡΠ΅Π΄ΡΡΠ² Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ ΡΠΈΡΠΎΡ ΡΠΎΠΌΠ° Π 450(51) Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π±Π΅Π»ΠΊΠ°-ΠΌΠΈΡΠ΅Π½ΠΈ
The development of the integral platform βFrom Gene to Leadβ, consolidated computer methods, bioinformatics researches, and experimental approaches, significantly accelerated and optimized base structure search in the field of drug design. The necessity of the experimental verification of hundreds virtual structure hypothesis (results of molecular data base selections or de novo construction) requires demands the usage of the high-through out and sensitive methods for validation possible interaction between numerous of selected compounds and particular molecular targets and evaluation of affinity, kinetics and thermodynamics. Surface plasmon resonance (SPR) technology makes it possible to solve all these problems. In this article the methodical aspects of the optical SPR-biosensor usage in the field of drug prototypes selection are described using the human cytochrome P450(51) catalyzing one of the key step of cholesterol biosynthesis as an example.ΠΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΡΡ
, Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ Π² Π΅Π΄ΠΈΠ½ΡΡ ΠΏΠ»Π°ΡΡΠΎΡΠΌΡ, ΠΏΠΎΠΊΡΡΠ²Π°ΡΡΡΡ ΠΏΡΡΡ βΠΎΡ Π³Π΅Π½Π° Π΄ΠΎ ΠΏΡΠΎΡΠΎΡΠΈΠΏΠ° Π»Π΅ΠΊΠ°ΡΡΡΠ²Π°β, Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΡΠΊΠΎΡΠΈΠ»Π° ΠΈ ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°Π»Π° ΠΏΠΎΠΈΡΠΊ Π±Π°Π·ΠΎΠ²ΡΡ
ΡΡΡΡΠΊΡΡΡ Π΄Π»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ Π½ΠΎΠ²ΡΡ
Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ². ΠΡΠΈ ΡΡΠΎΠΌ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΏΡΠΎΠ²Π΅ΡΠΊΠΈ Π½Π°ΠΉΠ΄Π΅Π½Π½ΡΡ
ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΡΠΎΡΠ΅Π½ ΡΡΡΡΠΊΡΡΡΠ½ΡΡ
Π³ΠΈΠΏΠΎΡΠ΅Π·, ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡΠΈΡ
ΡΠΎΠ±ΠΎΠΉ Π²ΡΠ±ΠΎΡΠΊΠΈ ΠΈΠ· ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
Π±Π°Π· Π΄Π°Π½Π½ΡΡ
ΠΈΠ»ΠΈ ΡΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
de novo ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, ΡΡΠ΅Π±ΡΠ΅Ρ ΠΏΡΠΈΠ²Π»Π΅ΡΠ΅Π½ΠΈΡ Π±ΡΡΡΡΡΡ
ΠΈ ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ΠΎΠ²ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π°Π½Π°Π»ΠΈΠ·Π° ΠΈΡ
Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΡ
Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ Ρ Π±Π΅Π»ΠΊΠΎΠΌ-ΠΌΠΈΡΠ΅Π½ΡΡ. Π Π² ΡΠ»ΡΡΠ°Π΅ ΠΏΠΎΠ·ΠΈΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ° ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΎΡΠ΅Π½ΠΊΠΈ Π°ΡΡΠΈΠ½Π½ΠΎΡΡΠΈ, ΠΊΠΈΠ½Π΅ΡΠΈΠΊΠΈ ΠΈ ΡΠ΅ΡΠΌΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ ΠΌΠ΅ΠΆΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ. Π’Π΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΠΎΠ³ΠΎ ΠΏΠ»Π°Π·ΠΌΠΎΠ½Π½ΠΎΠ³ΠΎ ΡΠ΅Π·ΠΎΠ½Π°Π½ΡΠ° (SPR) ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΠ΅ΡΠ°ΡΡ Π²ΡΠ΅ ΠΏΠ΅ΡΠ΅ΡΠΈΡΠ»Π΅Π½Π½ΡΠ΅ Π·Π°Π΄Π°ΡΠΈ. Π Π΄Π°Π½Π½ΠΎΠΉ ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π°ΡΠΏΠ΅ΠΊΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ SPR Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ° Π΄Π»Ρ ΠΏΠΎΠΈΡΠΊΠ° ΠΏΡΠΎΡΠΎΡΠΈΠΏΠΎΠ² Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΡΠ΅Π΄ΡΡΠ² Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° Π 450(51) ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΠΊΠ°ΡΠ°Π»ΠΈΠ·ΠΈΡΡΡΡΠ΅Π³ΠΎ ΠΊΠ»ΡΡΠ΅Π²ΡΡ ΡΡΠ°Π΄ΠΈΡ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° Ρ
ΠΎΠ»Π΅ΡΡΠ΅ΡΠΈΠ½Π°
Augmentative and Alternative Communication and Alleviating of the Behavioral Difficulties of Adolescents with Severe Multiple Developmental and Behavioral Disorders
This article is relevant in the framework of the study educational training importance and the ability to communicate with adolescents with severe multiple developmental and behavioral disorders (SMDD) in adults working for inpa- tient social service institution (ISSI). The survey of communication and behavioral skills in adolescents with SMDD living in ISSI presented. The study involved 60 employees, 28 adolescents with SMDD, 20 parents of special children. Methods used: Communication Matrix, cyclogram Β«Review of the dayΒ», evaluation sheet Β«Table of interestsΒ». A spe- cific case of employee training and introduction of Augmentative and Alternative Communication techniques into an individual form of work with the student and the impact of these activities on improving her behavior demonstrated. The experimental data presented in this article can be used for work with ISSI to increase the communication skills available to students with SMDD. Also, the article includes the description of the work on improvement of the qual- ity of life of adolescents with SMDD that are in inpatient institutional care. It has been established that children and adolescents with SMDD living in the ISSI have persistent impairments in mastering skills, identifying their com- munication mechanisms
ΠΡΠΎΡΠ΅ΠΎΠ»ΠΈΠΏΠΎΡΠΎΠΌΡ ΠΊΠ°ΠΊ ΡΠΏΠΎΡΠΎΠ± ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΡ Π±Π΅Π»ΠΊΠΎΠ² Π΄Π»Ρ SPR-Π°Π½Π°Π»ΠΈΠ·Π° Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ CYP3A4 ΠΈ CYB5A ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
Microsomal systems of human cytochrome P450 consist of three components, which are membrane proteins: cytochrome P450 hemoprotein (CYP), NADPH-dependent cytochrome P450 reductase (CPR), and a small regulatory heme-containing protein cytochrome bβ
(CYB5A). In the study of the cytochrome P450 system functioning the study of intermolecular interactions both with partner proteins and with possible drug prototypes is of great importance. Surface plasmon resonance (SPR) is a powerful and reliable tool for studying intermolecular interactions. However, there is a problem of immobilization of membrane proteins on the optical chip of the SPR biosensor. It is important to immobilize such proteins in native conditions with respect to the correct orientation of the protein globule to the surface of sensor. Previously, we have developed and described a method involving direct native immobilization of membrane proteins into a planar bilayer lipid membrane on the surface of a biosensor chip. At the same time, one of the commonly used approaches to working with membrane proteins using various methods is the construction of proteoliposomes containing membrane proteins. In this work, using CYP3A4 and CYB5A as protein partners, we evaluated two approaches to the creation of proteoliposomes: incorporation of a membrane protein into liposomes saturated with detergents and incorporation of a membrane protein into the forming proteoliposomes by the mechanism of micellar coalescence. The interaction of CYP3A4 with proteoliposomes obtained by incorporating CYB5A into detergent-saturated liposomes was shown. On the contrary, interaction between CYP3A4 and proteoliposomes containing CYB5A, obtained by the method of micellar coalescence, was not detected. Thus, it was shown that the incorporation of the membrane protein into liposomes saturated with a detergent was a more preferable method for working with an SPR biosensor as compared to the method of proteoliposomes formation by micellar coalescence. Detailed protocols for the creation of proteoliposomes and SPR-analysis can be useful to a wide range of researchers.ΠΠΈΠΊΡΠΎΡΠΎΠΌΠ°Π»ΡΠ½ΡΠ΅ ΡΠΈΡΡΠ΅ΠΌΡ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠΎΠ² P450 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΡΠΎΡΡΠΎΡΡ ΠΈΠ· ΡΡΡΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ², ΡΠ²Π»ΡΡΡΠΈΡ
ΡΡ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΠΌΠΈ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ: Π³Π΅ΠΌΠΎΠΏΡΠΎΡΠ΅ΠΈΠ½Π° ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° P450 (CYP), NADPH-Π·Π°Π²ΠΈΡΠΈΠΌΠΎΠΉ ΡΠΈΡΠΎΡ
ΡΠΎΠΌ Π 450 ΡΠ΅Π΄ΡΠΊΡΠ°Π·Ρ (CPR) ΠΈ Π½Π΅Π±ΠΎΠ»ΡΡΠΎΠ³ΠΎ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ Π³Π΅ΠΌ-ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠ΅Π³ΠΎ Π±Π΅Π»ΠΊΠ° ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ° b₅ (CYB5A). ΠΠ°ΠΆΠ½ΡΠΌ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ΠΌ Π² ΠΈΠ·ΡΡΠ΅Π½ΠΈΠΈ ΡΠΈΡΡΠ΅ΠΌΡ ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠΎΠ² Π 450 ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΠΆΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ ΠΊΠ°ΠΊ Ρ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ-ΠΏΠ°ΡΡΠ½ΡΡΠ°ΠΌΠΈ, ΡΠ°ΠΊ ΠΈ Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΠΌΠΈ ΠΏΡΠΎΡΠΎΡΠΈΠΏΠ°ΠΌΠΈ Π»Π΅ΠΊΠ°ΡΡΡΠ². ΠΠ΄Π½ΠΈΠΌ ΠΈΠ· ΠΏΠ΅ΡΠ΅Π΄ΠΎΠ²ΡΡ
ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠ² ΠΊ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΌΠ΅ΠΆΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΠΎΠ³ΠΎ ΠΏΠ»Π°Π·ΠΌΠΎΠ½Π½ΠΎΠ³ΠΎ ΡΠ΅Π·ΠΎΠ½Π°Π½ΡΠ° (SPR). ΠΡΠΈ ΡΡΠΎΠΌ Π²ΠΎΠ·Π½ΠΈΠΊΠ°Π΅Ρ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ° ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΡ
Π±Π΅Π»ΠΊΠΎΠ² Π½Π° ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠΈΠΏ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ°. ΠΠ»Ρ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π½Π°ΡΠΈΠ²Π½ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΠΉ Π²Π°ΠΆΠ½ΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ ΠΈΠΌΠ΅Π΅Ρ ΡΠΎΠ±Π»ΡΠ΄Π΅Π½ΠΈΠ΅ ΠΏΡΠ°Π²ΠΈΠ»ΡΠ½ΠΎΠΉ ΠΎΡΠΈΠ΅Π½ΡΠ°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠΉ Π³Π»ΠΎΠ±ΡΠ»Ρ Π² ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅. Π Π°Π½Π΅Π΅ Π½Π°ΠΌΠΈ Π±ΡΠ» ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΌΠ΅ΡΠΎΠ΄, ΠΏΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°ΡΡΠΈΠΉ ΠΏΡΡΠΌΡΡ ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΡ Π½Π°ΡΠΈΠ²Π½ΡΡ
ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΡ
Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΏΠ»Π°Π½Π°ΡΠ½ΡΡ Π±ΠΈΡΠ»ΠΎΠΉΠ½ΡΡ Π»ΠΈΠΏΠΈΠ΄Π½ΡΡ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Ρ Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΡΠΈΠΏΠ° Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠ°. ΠΡΡΠ³ΠΈΠΌ ΡΠΈΡΠΎΠΊΠΎ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½ΡΠ½Π½ΡΠΌ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠΌ Π΄Π»Ρ ΡΠ°Π±ΠΎΡΡ Ρ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΠΌΠΈ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΊΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΠΏΠΎΡΠΎΠΌ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ. Π Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ Π½Π°ΠΌΠΈ Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΠΏΠ°ΡΡΠ½ΡΡΠΎΠ² CYP3A4 ΠΈ CYB5A Π±ΡΠ»ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ Π΄Π²ΡΡ
ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠ² ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΠΏΠΎΡΠΎΠΌ Π΄Π»Ρ SPR-Π°Π½Π°Π»ΠΈΠ·Π° ΠΌΠ΅ΠΆΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΡΡ
Π±Π΅Π»ΠΊΠΎΠ²: Π²ΡΡΡΠ°ΠΈΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° Π² Π»ΠΈΠΏΠΎΡΠΎΠΌΡ, Π½Π°ΡΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π΅ΡΠ΅ΡΠ³Π΅Π½ΡΠΎΠΌ, ΠΈ Π²ΡΡΡΠ°ΠΈΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° Π² ΡΠΎΡΠΌΠΈΡΡΡΡΠΈΠ΅ΡΡ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΠΏΠΎΡΠΎΠΌΡ ΠΏΠΎ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡ ΠΌΠΈΡΠ΅Π»Π»ΡΡΠ½ΠΎΠΉ ΠΊΠΎΠ°Π»Π΅ΡΡΠ΅Π½ΡΠΈΠΈ. SPR-Π°Π½Π°Π»ΠΈΠ· ΠΏΠΎΠΊΠ°Π·Π°Π» Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ CYP3A4 Ρ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΠΏΠΎΡΠΎΠΌΠ°ΠΌΠΈ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π²ΡΡΡΠ°ΠΈΠ²Π°Π½ΠΈΡ CYB5A Π² Π»ΠΈΠΏΠΎΡΠΎΠΌΡ, Π½Π°ΡΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π΅ΡΠ΅ΡΠ³Π΅Π½ΡΠΎΠΌ. Π€Π°ΠΊΡ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ CYP3A4 ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΌΠΈΡΠ΅Π»Π»ΡΡΠ½ΠΎΠΉ ΠΊΠΎΠ°Π»Π΅ΡΡΠ΅Π½ΡΠΈΠΈ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΠΏΠΎΡΠΎΠΌΠ°ΠΌΠΈ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΌΠΈ CYB5A, Π·Π°ΡΠΈΠΊΡΠΈΡΠΎΠ²Π°ΡΡ Π½Π΅ ΡΠ΄Π°Π»ΠΎΡΡ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, ΡΡΠΎ Π²ΡΡΡΠ°ΠΈΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° Π² Π»ΠΈΠΏΠΎΡΠΎΠΌΡ, Π½Π°ΡΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π΅ΡΠ΅ΡΠ³Π΅Π½ΡΠΎΠΌ, ΡΠ²Π»ΡΠ΅ΡΡΡ Π±ΠΎΠ»Π΅Π΅ ΠΏΡΠ΅Π΄ΠΏΠΎΡΡΠΈΡΠ΅Π»ΡΠ½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π΄Π»Ρ ΡΠ°Π±ΠΎΡΡ Ρ SPR-Π±ΠΈΠΎΡΠ΅Π½ΡΠΎΡΠΎΠΌ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΠΏΠΎΡΠΎΠΌ ΠΌΠΈΡΠ΅Π»Π»ΡΡΠ½ΠΎΠΉ ΠΊΠΎΠ°Π»Π΅ΡΡΠ΅Π½ΡΠΈΠ΅ΠΉ. ΠΡΠΈΠ²Π΅Π΄Π΅Π½Π½ΡΠ΅ ΠΏΠΎΠ΄ΡΠΎΠ±Π½ΡΠ΅ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΠΎΠ»ΠΈΠΏΠΎΡΠΎΠΌ ΠΈ SPR-Π°Π½Π°Π»ΠΈΠ·Π° ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΏΠΎΠ»Π΅Π·Π½Ρ ΡΠΈΡΠΎΠΊΠΎΠΌΡ ΠΊΡΡΠ³Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»Π΅ΠΉ