Antimicrobial properties of chlorophyll and hemin incorporated into the polymeric matrix of poly-N-vinylpyrrolidone

The increase in the number of antibiotic-resistant strains of microorganisms is becoming more widespread. Metalloporphyrins are promising and modern antimicrobial agents. The most well-known representatives of metalloporphyrins are chlorophyll (Chl) and hemin. This paper presents the results of studies on the effectiveness of Chl and hemin complexes with poly-N -vinylpyrrolidone (PVP) as an antimicrobial agent against Staphylococcus aureus and Escherichia coli. The method for preparing polymeric forms of Chl and hemin is presented. The binding constants of these substances to the polymer were calculated, which were 0.5×105 L/mol for Chl and 3.3×104 L/mol for hemin. Experimental data on the release of substances from the polymeric matrix were obtained. It was found that the complete release of Chl from PVP was observed after 13 h, and hemin – after 10 h. The data on the comparative antimicrobial effect of substances in free and polymeric form were obtained in a microbiological test. Further these results can be used in the development of medicines against microbial infections

Phase formation during firing of elevated-basicity SSGPO pellets

26.00; Translated from Russian (Stal' 1987 (11) p. 17-20)Available from British Library Document Supply Centre- DSC:9022.06(BISI-Trans--26568)T / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo

Abnormal promoter DNA hypermethylation of the integrin, nidogen, and dystroglycan genes in breast cancer

Cell transmembrane receptors and extracellular matrix components play a pivotal role in regulating cell activity and providing for the concerted integration of cells in the tissue structures. We have assessed DNA methylation in the promoter regions of eight integrin genes, two nidogen genes, and the dystroglycan gene in normal breast tissues and breast carcinomas (BC). The protein products of these genes interact with the basement membrane proteins LAMA1, LAMA2, and LAMB1; abnormal hypermethylation of the LAMA1, LAMA2, and LAMB1 promoters in BC has been described in our previous publications. In the present study, the frequencies of abnormal promoter hypermethylation in BC were 13% for ITGA1, 31% for ITGA4, 4% for ITGA7, 39% for ITGA9, 38% for NID1, and 41% for NID2. ITGA2, ITGA3, ITGA6, ITGB1, and DAG1 promoters were nonmethylated in normal and BC samples. ITGA4, ITGA9, and NID1 promoter hypermethylation was associated with the HER2 positive tumors, and promoter hypermethylation of ITGA1, ITGA9, NID1 and NID2 was associated with a genome-wide CpG island hypermethylated BC subtype. Given that ITGA4 is not expressed in normal breast, one might suggest that its abnormal promoter hypermethylation in cancer is non-functional and is thus merely a passenger epimutation. Yet, this assumption is not supported by our finding that it is not associated with a hypermethylated BC subtype. ITGA4 acquires expression in a subset of breast carcinomas, and methylation of its promoter may be preventive against expression in some tumors. Strong association of abnormal ITGA4 hypermethylation with the HER2 positive tumors (p = 0.0025) suggests that simultaneous presence of both HER2 and integrin α4 receptors is not beneficial for tumor cells. This may imply HER2 and integrin α4 signaling pathways interactions that are yet to be discovered. © 2021, The Author(s)