A comparative study of hydrophilic phosphine hexanuclear rhenium cluster complexes’ toxicity

Octahedral rhenium cluster compound Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] has recently emerged as a very promising X-ray contrast agent for biomedical applications. However, the synthesis of this compound is rather challenging due to difficulty to control the hydrolysis of initial P(C2H4CN)3 ligand during the reaction process. Therefore, in this report we compare the in vitro and in vivo toxicity of Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] with those of related compounds featuring fully hydrolysed form of the phosphine ligand, namely Na2H14[{Re6Q8}(P(C2H4COO)3)6] (Q = S or Se). Our results demonstrate that cytotoxicity and acute in vivo toxicity of the complex Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] solutions were considerably lower than those of compounds with fully hydrolysed ligand P(C2H4COOH)3. Such behavior can be explained by the higher osmolality of Na2H14[{Re6Q8}(P(C2H4COO)3)6] versus Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6]

Cellular imaging by green luminescence of Tb(III)-doped aminomodified silica nanoparticles

© 2017 Elsevier B.V.The work introduces Tb(III)-centered luminescence of amino-modified silica nanoparticles doped with Tb(III) complexes for cellular imaging. For these purposes water-in-oil procedure was optimized for synthesis of 20 and 35 nm luminescent nanoparticles with amino-groups embedded on the surface. The obtained results indicate an impact of the nanoparticle size in decoration, aggregation behavior and luminescent properties of the nanoparticles in protein-based buffer solutions. Formation of a protein-based corona on the nanoparticles surface was revealed through the effect of the nanoparticles on helical superstructure of BSA. This effect is evident from CD spectral data, while no any size impact on the adsorption of BSA onto aminomodified silica surface was observed. Cellular uptake of the nanoparticles studied by confocal and TEM microscopy methods indicates greater cellular uptake for the smaller nanoparticles. Cytotoxicity of the nanoparticles was found to agree well with their cellular uptake behavior, which in turn was found to be greater for the smaller nanoparticles

СРАВНИТЕЛЬНЫЙ АНАЛИЗ ДВУХЭНЕРГЕТИЧЕСКОЙ РЕНТГЕНОВСКОЙ АБСОРБЦИОМЕТРИИ И БИОИМПЕДАНСОМЕТРИИ В ОЦЕНКЕ КОМПОНЕНТНОГО СОСТАВА ТЕЛА ПАЦИЕНТОВ НА ПРОГРАММНОМ ГЕМОДИАЛИЗЕ

The aim of the study. Compare the efficiency of the use of dual-energy x-ray absorptiometry (DRA) and bioimpedansometry (BIM) in the evaluation of the body composition of hemodialysis patients.Patients and methods. 67 patients on hemodialysis, among which 23 men and 44 women aged 54±14,4 years. BIM and DRA were used to evaluate the body composition.Results. The results of determining the total muscle mass of the body obtained by the results of DFA and BIM were compared with the use of the Bland-Altman method. The correlation coefficient between the indices was R=0,994, p<0,0001, delta (M±s) was –0,48±0,91 kg, CI 95% (–0,71)…(–0,26) kg.Conclusion. DRA does not have significant advantages over tetrapolar multifrequency BIM when assessing the body composition in hemodialysis patients. Цель исследования: сравнить эффективность использования двухэнергетической рентгеновской абсорбциометрии (ДРА) и биоимпедансометрии (БИМ) в оценке компонентного состава тела гемодиализных пациентов.Материалы и методы: обследовано 67 пациентов, получающих лечение программным гемодиализом (ГД), среди них 23 мужчины и 44 женщины, средний возраст 54±14,4 года. Для оценки компонентного состава тела использовали БИМ и ДРА.Результаты. Результаты определения общей мышечной массы тела, полученной по результатам ДРА и БИМ сравнили с использованием метода Блэнда–Альтмана. Коэффициент корреляции между показателями составил r=0,994, p<0,0001, дельта (M±s) составила –0,48±0,91 кг, ДИ 95% (–0,71)… (–0,26) кг.Заключение: ДРА не имеет значимых преимуществ по сравнению с тетраполярной мультичастотной БИМ при оценке компонентного состава тела у больных на гемодиализе.

Cellular imaging by green luminescence of Tb(III)-doped aminomodified silica nanoparticles

© 2017 Elsevier B.V.The work introduces Tb(III)-centered luminescence of amino-modified silica nanoparticles doped with Tb(III) complexes for cellular imaging. For these purposes water-in-oil procedure was optimized for synthesis of 20 and 35 nm luminescent nanoparticles with amino-groups embedded on the surface. The obtained results indicate an impact of the nanoparticle size in decoration, aggregation behavior and luminescent properties of the nanoparticles in protein-based buffer solutions. Formation of a protein-based corona on the nanoparticles surface was revealed through the effect of the nanoparticles on helical superstructure of BSA. This effect is evident from CD spectral data, while no any size impact on the adsorption of BSA onto aminomodified silica surface was observed. Cellular uptake of the nanoparticles studied by confocal and TEM microscopy methods indicates greater cellular uptake for the smaller nanoparticles. Cytotoxicity of the nanoparticles was found to agree well with their cellular uptake behavior, which in turn was found to be greater for the smaller nanoparticles

СРАВНИТЕЛЬНЫЙ АНАЛИЗ ДВУХЭНЕРГЕТИЧЕСКОЙ РЕНТГЕНОВСКОЙ АБСОРБЦИОМЕТРИИ И БИОИМПЕДАНСОМЕТРИИ В ОЦЕНКЕ КОМПОНЕНТНОГО СОСТАВА ТЕЛА ПАЦИЕНТОВ НА ПРОГРАММНОМ ГЕМОДИАЛИЗЕ

The aim of the study. Compare the efficiency of the use of dual-energy x-ray absorptiometry (DRA) and bioimpedansometry (BIM) in the evaluation of the body composition of hemodialysis patients.Patients and methods. 67 patients on hemodialysis, among which 23 men and 44 women aged 54±14,4 years. BIM and DRA were used to evaluate the body composition.Results. The results of determining the total muscle mass of the body obtained by the results of DFA and BIM were compared with the use of the Bland-Altman method. The correlation coefficient between the indices was R=0,994, p&lt;0,0001, delta (M±s) was –0,48±0,91 kg, CI 95% (–0,71)…(–0,26) kg.Conclusion. DRA does not have significant advantages over tetrapolar multifrequency BIM when assessing the body composition in hemodialysis patients. Цель исследования: сравнить эффективность использования двухэнергетической рентгеновской абсорбциометрии (ДРА) и биоимпедансометрии (БИМ) в оценке компонентного состава тела гемодиализных пациентов.Материалы и методы: обследовано 67 пациентов, получающих лечение программным гемодиализом (ГД), среди них 23 мужчины и 44 женщины, средний возраст 54±14,4 года. Для оценки компонентного состава тела использовали БИМ и ДРА.Результаты. Результаты определения общей мышечной массы тела, полученной по результатам ДРА и БИМ сравнили с использованием метода Блэнда–Альтмана. Коэффициент корреляции между показателями составил r=0,994, p&lt;0,0001, дельта (M±s) составила –0,48±0,91 кг, ДИ 95% (–0,71)… (–0,26) кг.Заключение: ДРА не имеет значимых преимуществ по сравнению с тетраполярной мультичастотной БИМ при оценке компонентного состава тела у больных на гемодиализе. </p

Cellular imaging by green luminescence of Tb(III)-doped aminomodified silica nanoparticles

© 2017 Elsevier B.V.The work introduces Tb(III)-centered luminescence of amino-modified silica nanoparticles doped with Tb(III) complexes for cellular imaging. For these purposes water-in-oil procedure was optimized for synthesis of 20 and 35 nm luminescent nanoparticles with amino-groups embedded on the surface. The obtained results indicate an impact of the nanoparticle size in decoration, aggregation behavior and luminescent properties of the nanoparticles in protein-based buffer solutions. Formation of a protein-based corona on the nanoparticles surface was revealed through the effect of the nanoparticles on helical superstructure of BSA. This effect is evident from CD spectral data, while no any size impact on the adsorption of BSA onto aminomodified silica surface was observed. Cellular uptake of the nanoparticles studied by confocal and TEM microscopy methods indicates greater cellular uptake for the smaller nanoparticles. Cytotoxicity of the nanoparticles was found to agree well with their cellular uptake behavior, which in turn was found to be greater for the smaller nanoparticles

Cellular imaging by green luminescence of Tb(III)-doped aminomodified silica nanoparticles

© 2017 Elsevier B.V.The work introduces Tb(III)-centered luminescence of amino-modified silica nanoparticles doped with Tb(III) complexes for cellular imaging. For these purposes water-in-oil procedure was optimized for synthesis of 20 and 35 nm luminescent nanoparticles with amino-groups embedded on the surface. The obtained results indicate an impact of the nanoparticle size in decoration, aggregation behavior and luminescent properties of the nanoparticles in protein-based buffer solutions. Formation of a protein-based corona on the nanoparticles surface was revealed through the effect of the nanoparticles on helical superstructure of BSA. This effect is evident from CD spectral data, while no any size impact on the adsorption of BSA onto aminomodified silica surface was observed. Cellular uptake of the nanoparticles studied by confocal and TEM microscopy methods indicates greater cellular uptake for the smaller nanoparticles. Cytotoxicity of the nanoparticles was found to agree well with their cellular uptake behavior, which in turn was found to be greater for the smaller nanoparticles