Fatty acids composition of Bacillus subtilis ONU551 lipids

The aim of the study was to determine the cellular lipids fatty acid composition for identification of the Bacillus subtilis ONU551 strain bacteria, which is a phenol destructor. Fatty acids analysis of B. subtilis ONU551 strain was performed using an automatic system for microorganisms’ identification MIDI Sherlock (MIDI, USA) based on gas chromatograph Agilent 7890. Chromatograms analysis showed that the fatty acid spectrum of the strain B. subtilis ONU551 consisted predominately of branched structural isomers of saturated acids: 13-methyltetradecanoic (15:0 iso; 34.72%) and 12-methyltetradecanoic (15:0 anteiso; 33.72%) acids. The total content of the branched saturated fatty acids was 88.16% – 14:0 iso (0.52%), 15:0 iso (34.72%), 15:0 anteiso (33.72%), 16:0 iso (1.85%), 17:0 iso (7.11%), 17:0 anteiso (10.24%). The saturated fatty acids of the normal structure were also detected – 12:0 (0.36%), 14:0 (0.28%), 16:0 (1.30%). No 2- and 3-hydroxy acids and no cyclic fatty acids were detected in the fatty acid profile of B. subtilis ONU551 strain. Unsaturated fatty acid isomers – 15:1 w5c (1.85%), 16:1 w11c (1.21%), 16:1 w7c alcohol (1.08%), 17:1 iso w10c (3.18%), ∑17:1 iso I/anteiso B (2.57%) were shown to be the distinctive biomarkers of the B. subtilis ONU551 strain. According to the fatty acid profile analysis with MIDI Sherlock system, the studied strain was identified as Bacillus subtilis with high level of similarity index (0.563)

Cellular fatty acid composition of Aeromonas genus – destructor of aromatic xenobiotics

The aim of this study was a determination of the fatty acid composition of cellular lipids and identification of the strains, isolated from the wastewater of pharmaceutical production, – the destructor of aromatic xenobiotics. The phenotypic characteristics and cellular fatty acid (FA) composition confirmed the strain belonging to the Aeromonas ichthiosmia with the similarity index of library data MIDI Sherlock – 0.564. Analysis of the cellular FA composition of the strain Aeromonas ichthiosmia ONU552 was carried out using the MIDI Sherlock microorganism identification system based on the gas chromatograph Agilent 7890. Chromatographic analysis showed that the fatty acid profile of the strain Aeromonas ichthiosmia ONU552 contains 26 fatty acids with the total number of carbon atoms from 10 to 18. 85.27% of saturated and unsaturated fatty acids had unbranched structure. The total content of unsaturated fatty acids – 16:1 w7c/16:1 w6c, 18:1 w7c, 16:1 w7c alcohol, 17:1 w8c, 17:1 w6c, 16:1 w5c, was 50% of the total fatty acid pool. Less than 1.5% branched fatty acids were predominantly in the iso form: 13:0 iso (0.20%); 15:0 iso (0.97%); 17:1 iso w9c (1.35%), 17:0 iso (1.49%); in the anteiso form, only one acid 17:0 (0.27%) was identified. It was shown that the characteris­tic of the fatty acid composition of the strain Aeromonas ichthiosmia ONU552 – the destructor of aromatic xenobio­tics, was the presence of hydroxyacids 12:0 3OH, 15:0 3OH, 15:0 iso 3OH and dominance of hexadecanoic (16:0) and hexadecenoic (16:1 w7c/16:1 w6c) of fatty acids

THE ANALYSIS OF ADSORPTION OF HEXADECYLPYRIDINIUM BROMIDES AND SODIUM ALKYLSULPHATES ON THE BINARY SOLUTION OF SURFACTANT — AIR INTERFACE

It is experimentally shown, that the diluted binary solutions of hexadecylpyridinium bromides and sodium alkylsulphates (sodium decyl- and dodecylsulphates) find out synergism concerning decrease in a surface tension. The opportunity of definition of structure mixed adsorption layers by a method of the Rosen in which basis the theory of regular solutions lays, and the method of Hutchinson based on the equation of adsorption Gibbs is shown. Process of adsorption of surfactants of their binary solutions is thermodynamically more favourable process, than of adsorption of surfactants of individual solutions

INTENSIFICATION OF FLOTATION EXCEPTION OF NONIONIC SURFACTANT

The influence of polietilenglikol, geksacianoferat (III) potassium and geksacianoferat (II) potassium on the flotation exception of non-ionic surfactant are studied

ІНТЕНСИФІКАЦІЯ ФЛОТАЦІЙНОГО ВИЛУЧЕННЯ НЕІОНОГЕННИХ ПОВЕРХНЕВО-АКТИВНИХ РЕЧОВИН

The influence of polietilenglikol, geksacianoferat (III) potassium and geksacianoferat (II) potassium on the flotation exception of non-ionic surfactant are studied.Изучены закономерности флотационного извлечения неионогенных ПАВ(НПАВ) с помощью добавок полиэтиленгликоля (ПЭГ) и неорганических солей.Определены параметры мицеллообразования и адсорбции на границе раздела фаз раствор–воздух Твина-21, ПЭГ и их смеси. Предложен механизм взаимодействия и образования адсорбционных слоев в системе Твин-21 – ПЭГ. Определены оптимальные условия проведения процесса флотационного извлечения НПАВ (природа НПАВ, состав раствора, значение рН среды).Вивчено вплив поліетиленгліколю, гексаціаноферату (III) калію та гексаціаноферату (II) калію на флотаційне вилучення неіоногенних поверхнево-активних речовин