17 research outputs found
Cytotoxicity assessment of heparin nanoparticles in NR8383 macrophages
International audienceThe bioavailability of low molecular weight heparin (LMWH) has been increased by encapsulation in nanoparticles. As a complement to these results, the cytotoxicity and apoptosis induced by LMWH nanoparticles prepared by two methods [nanoprecipitation (NP) and double emulsion (DE)] using EudragitÂź RS (RS) and poly-É-caprolactone (PCL) have been analysed. Particle sizes varied from 54 to 400 nm with zeta potential values between â65 and +63 mV. Our results showed that the method of nanoparticle preparation affects their properties, especially in terms of drug incorporation and cell tolerance. Cell viability ranged from 6% to 100% depending on the preparation method and physicochemical properties of the particles and the type of toxicity assay. Particle diameter and zeta potential seemed to be the most valuable cytotoxicity markers when cell viability was measured by Trypan blue exclusion and MTT respectively. Nanoparticles prepared by DE were better tolerated than those of NP. LMWH encapsulation into the cationic nanoparticles reduces remarkably their toxicity. Apoptosis evaluation showed activated caspases in exposed cells. However, no nuclear fragmentation was detected in NR8383 cells whatever the tested nanoparticles. DE nanoparticles of RS and PCL can be proposed as a good LMWH delivery system due to their low toxicity (IC50 ⌠2.33 and 0.96 mg/mL, respectively)
Zeta-potential and morphology of electrospun nano- and microfibers from biopolymers and their blends used as scaffolds in tissue engineering
Zeta-potential and morphology of electrospun nano- and microfibers from biopolymers and their blends used as scaffolds in tissue engineering
Industrial Policy and Competition Enforcement: Is There, Could There and Should There Be a Nexus?
The role of ATP as a mediator in the action of iron complexes on cellular calcium homeostasis
Identification of three clinical neurofibromatosis 1 subtypes: Latent class analysis of a series of 1351 patients
International audienc