Abundant and equipotent founder cells establish and maintain acute lymphoblastic leukaemia

High frequencies of blasts in primary acute lymphoblastic leukaemia (ALL) samples have the potential to induce leukaemia and to engraft mice. However, it is unclear how individual ALL cells each contribute to drive leukaemic development in a bulk transplant and the extent to which these blasts vary functionally. We used cellular barcoding as a fate mapping tool to track primograft ALL blasts in vivo. Our results show that high numbers of ALL founder cells contribute at similar frequencies to leukaemic propagation over serial transplants, without any clear evidence of clonal succession. These founder cells also exhibit equal capacity to home and engraft to different organs, although stochastic processes may alter the composition in restrictive niches. Our findings enhance the stochastic stem cell model of ALL by demonstrating equal functional abilities of singular ALL blasts and show that successful treatment strategies must eradicate the entire leukaemic cell population

The squeezed generalized amplitude damping channel

Squeezing of a thermal bath introduces new features absent in an open quantum system interacting with an uncorrelated (zero squeezing) thermal bath. The resulting dynamics, governed by a Lindblad-type evolution, extends the concept of a generalized amplitude damping channel, which corresponds to a dissipative interaction with a purely thermal bath. Here we present the Kraus representation of this map, which we call the squeezed generalized amplitude damping channel. As an application of this channel to quantum information, we study the classical capacity of this channel.Comment: Accepted for publication in Physical Review A; note on methods of generating bath squeezing added in Section II A; 12 pages, 7 figure

Cemetery Inscriptions - Village Cemetery and Elm Grove Cemetery, Thomaston, Maine

Compiled by Edith H. Wilson, Maria O. Hawley, and Harriet R. Williams of the General Knox Chapter of the Maine Society of the Daughters of the American Revolution during the Summer and Fall of 1956.https://digitalmaine.com/books/1036/thumbnail.jp

Short-term studies underestimate 30-generation changes in a butterfly metapopulation

Most studies of rare and endangered species are based on work carried out within one generation, or over one to a few generations of the study organism. We report the results of a study that spans 30 generations (years) of the entire natural range of a butterfly race that is endemic to 35 km2 of north Wales, UK. Short-term studies (surveys in single years and dynamics over 4 years) of this system led to the prediction that the regional distribution would be quite stable, and that colonization and extinction dynamics would be relatively unimportant. However, a longer-term study revealed unexpectedly high levels of population turnover (local extinction and colonization), affecting 18 out of the 20 patches that were occupied at any time during the period. Modelling the system (using the 'incidence function model' (IFM) for metapopulations) also showed higher levels of colonization and extinction with increasing duration of the study. The longer-term dynamics observed in this system can be compared, at a metapopulation level, with the increased levels of variation observed with increasing time that have been observed in single populations. Long-term changes may arise from local changes in the environment that make individual patches more or less suitable for the butterfly, or from unusual colonization or extinction events that take metapopulations into alternative states. One implication is that metapopulation and population viability analyses based on studies that cover only a few animal or plant generations may underestimate extinction threats

Scaling Laws for e+e−e^+ e^- Linear Colliders

Design studies of a future TeV e+e- Linear Collider (TLC) are presently being made by five major laboratories within the framework of a world-wide collaboration. A figure of merit is defined which enables an objective comparison of these different designs. This figure of merit is shown to depend only on a small number of parameters. General scaling laws for the main beam parameters and linac parameters are derived and prove to be very effective when used as guidelines to optimize the linear collider design. By adopting appropriate parameters for beam stability, the figure of merit becomes nearly independent of accelerating gradient and RF frequency of the accelerating structures. In spite of the strong dependence of the wake-fields with frequency, the single bunch emittance preservation during acceleration along the linac is also shown to be independent of the RF frequency when using equivalent trajectory correction schemes. In this situation, beam acceleration using high frequency structures becomes very advantageous because it enables high accelerating fields to be obtained, which reduces the overall length and consequently the total cost of the linac

Recent Outbursts from the Transient X-Ray Pulsar Cep X-4 (GS 2138+56)

We report on X-ray observations of the 66 s period transient X-ray pulsar Cep X-4 (GS 2138+56) with the Burst and Transient Source Experiment (BATSE) on the Compton Gamma-Ray Observatory (CGRO) and with the Rossi X-ray Timing Explorer (RXTE). Two outbursts from Cep X-4 were observed with BATSE in 1993 June-July and 1997 July. Pulse frequencies of 15.0941 +/- 0.0002 mHz on 1993 June 25 (MJD 49,163) and 15.0882 +/- 0.0002 mHz on 1997 July 12 (MJD 50,641) were each measured from 2 day spans of BATSE data near each outburst's peak. Cep X-4 showed an average spin down rate of (-4.14 +/- 0.08)*10^(-14) Hz/s between the 1993 and 1997 outbursts. After BATSE could no longer detect Cep X-4, public observations were performed on 1997 July 18 & 25 with the Proportional Counter Array (PCA) on RXTE. A pulse frequency of 15.088 +/- 0.004 mHz was measured from observations on 1997 July 18 (MJD 50,647). Significant aperiodic noise, with an rms variance of ~18% in the frequency range 0.01-1.0 Hz was observed on both days. Energy and intensity dependent pulse shape variations were also seen in these data. Recently published optical observations associate Cep X-4 with a Be companion star. If all 4 outbursts observed from Cep X-4 are assumed to occur at the same orbital phase, we find that the orbital period is between 23 days and 147.3 days.Comment: 19 pages (LaTeX) including 9 figures. Accepted for publication in the Astrophysical Journa

Nanoscale Mechanical Drumming Visualized by 4D Electron Microscopy

With four-dimensional (4D) electron microscopy, we report in situ imaging of the mechanical drumming of a nanoscale material. The single crystal graphite film is found to exhibit global resonance motion that is fully reversible and follows the same evolution after each initiating stress pulse. At early times, the motion appears “chaotic” showing the different mechanical modes present over the micron scale. At longer time, the motion of the thin film collapses into a well-defined fundamental frequency of 1.08 MHz, a behavior reminiscent of mode locking; the mechanical motion damps out after ∼200 μs and the oscillation has a “cavity” quality factor of 150. The resonance time is determined by the stiffness of the material, and for the 75 nm thick and 40 μm square specimen used here we determined Young’s modulus to be 1.0 TPa for the in-plane stress−strain profile. Because of its real-time dimension, this 4D microscopy should have applications in the study of these and other types of materials structures