3,530 research outputs found
Two Distant Halo Velocity Groups Discovered by the Palomar Transient Factory
We report the discovery of two new halo velocity groups (Cancer groups A and B) traced by 8 distant RR Lyrae stars and observed by the Palomar Transient Factory (PTF) survey at R.A.~129Ā°, Dec~20Ā° (l~205Ā°, b~32Ā°). Located at 92 kpc from the Galactic center (86 kpc from the Sun), these are some of the most distant substructures in the Galactic halo known to date. Follow-up spectroscopic observations with the Palomar Observatory 5.1-m Hale telescope and W. M. Keck Observatory 10-m Keck I telescope indicate that the two groups are moving away from the Galaxy at v_(gsr) = 78.0+-5.6 km s^(-1) (Cancer group A) and v_(gsr) = 16.3+-7.1 km s^(-1) (Cancer group B). The groups have velocity dispersions of Ļ_(v_)gsr))=12.4+-5.0 km s^(-1) and Ļ _(v_(gsr))=14.9+-6.2 km s^(-1), and are spatially extended (about several kpc) making it very unlikely that they are bound systems, and are more likely to be debris of tidally disrupted dwarf galaxies or globular clusters. Both groups are metal-poor (median metallicities of [Fe/H]^A = -1.6 dex and [Fe/H]^B =-2.1 dex), and have a somewhat uncertain (due to small sample size) metallicity dispersion of ~0.4 dex, suggesting dwarf galaxies as progenitors. Two additional RR Lyrae stars with velocities consistent with those of the Cancer groups have been observed ~25 Ā° east, suggesting possible extension of the groups in that direction
Tracing the Orphan Stream to 55 kpc with RR Lyrae Stars
We report positions, velocities and metallicities of 50 ab-type RR Lyrae
(RRab) stars observed in the vicinity of the Orphan stellar stream. Using about
30 RRab stars classified as being likely members of the Orphan stream, we study
the metallicity and the spatial extent of the stream. We find that RRab stars
in the Orphan stream have a wide range of metallicities, from -1.5 dex to -2.7
dex. The average metallicity of the stream is -2.1 dex, identical to the value
obtained by Newberg et al. (2010) using blue horizontal branch stars. We find
that the most distant parts of the stream (40-50 kpc from the Sun) are about
0.3 dex more metal-poor than the closer parts (within ~30 kpc), suggesting a
possible metallicity gradient along the stream's length. We have extended the
previous studies and have mapped the stream up to 55 kpc from the Sun. Even
after a careful search, we did not identify any more distant RRab stars that
could plausibly be members of the Orphan stream. If confirmed with other
tracers, this result would indicate a detection of the end of the leading arm
of the stream. We have compared the distances of Orphan stream RRab stars with
the best-fit orbits obtained by Newberg et al. (2010). We find that model 6 of
Newberg et al. (2010) cannot explain the distances of the most remote Orphan
stream RRab stars, and conclude that the best fit to distances of Orphan stream
RRab stars and to the local circular velocity is provided by potentials where
the total mass of the Galaxy within 60 kpc is M_{60}~2.7x10^{11} Msun, or about
60% of the mass found by previous studies. More extensive modelling that would
consider non-spherical potentials and the possibility of misalignment between
the stream and the orbit, is highly encouraged.Comment: Submitted to ApJ, 15 pages in emulateapj format, three tables in
machine-readable format (download "Source" from "Other formats"
Insulin-Producing Cells Generated from Dedifferentiated Human Pancreatic Beta Cells Expanded In Vitro
Expansion of beta cells from the limited number of adult human islet donors is an attractive prospect for increasing cell availability for cell therapy of diabetes. However, attempts at expanding human islet cells in tissue culture result in loss of beta-cell phenotype. Using a lineage-tracing approach we provided evidence for massive proliferation of beta-cell-derived (BCD) cells within these cultures. Expansion involves dedifferentiation resembling epithelial-mesenchymal transition (EMT). Epigenetic analyses indicate that key beta-cell genes maintain open chromatin structure in expanded BCD cells, although they are not transcribed. Here we investigated whether BCD cells can be redifferentiated into beta-like cells.Redifferentiation conditions were screened by following activation of an insulin-DsRed2 reporter gene. Redifferentiated cells were characterized for gene expression, insulin content and secretion assays, and presence of secretory vesicles by electron microscopy. BCD cells were induced to redifferentiate by a combination of soluble factors. The redifferentiated cells expressed beta-cell genes, stored insulin in typical secretory vesicles, and released it in response to glucose. The redifferentiation process involved mesenchymal-epithelial transition, as judged by changes in gene expression. Moreover, inhibition of the EMT effector SLUG (SNAI2) using shRNA resulted in stimulation of redifferentiation. Lineage-traced cells also gave rise at a low rate to cells expressing other islet hormones, suggesting transition of BCD cells through an islet progenitor-like stage during redifferentiation.These findings demonstrate for the first time that expanded dedifferentiated beta cells can be induced to redifferentiate in culture. The findings suggest that ex-vivo expansion of adult human islet cells is a promising approach for generation of insulin-producing cells for transplantation, as well as basic research, toxicology studies, and drug screening
Aluminium and fluoride in drinking water in relation to later dementia risk
BACKGROUND: Environmental risk factors for dementia are poorly understood. Aluminium and fluorine in drinking water have been linked with dementia but uncertainties remain about this relationship. AIMS: In the largest longitudinal study in this context, we set out to explore the individual effect of aluminium and fluoride in drinking water on dementia risk and, as fluorine can increase absorption of aluminium, we also examine any synergistic influence on dementia. METHOD: We used Cox models to investigate the association between mean aluminium and fluoride levels in drinking water at their residential location (collected 2005ā2012 by the Drinking Water Quality Regulator for Scotland) with dementia in members of the Scottish Mental Survey 1932 cohort who were alive in 2005. RESULTS: A total of 1972 out of 6990 individuals developed dementia by the linkage date in 2012. Dementia risk was raised with increasing mean aluminium levels in women (hazard ratio per s.d. increase 1.09, 95% CI 1.03ā1.15, P < 0.001) and men (1.12, 95% CI 1.03ā1.21, P = 0.004). A dose-response pattern of association was observed between mean fluoride levels and dementia in women (1.34, 95% CI 1.28ā1.41, P < 0.001) and men (1.30, 95% CI 1.22ā1.39, P < 0.001), with dementia risk more than doubled in the highest quartile compared with the lowest. There was no statistical interaction between aluminium and fluoride levels in relation with dementia. CONCLUSIONS: Higher levels of aluminium and fluoride were related to dementia risk in a population of men and women who consumed relatively low drinking-water levels of both. DECLARATION OF INTEREST: NONE
Epidermal Growth FactorāPEG Functionalized PAMAM-Pentaethylenehexamine Dendron for Targeted Gene Delivery Produced by Click Chemistry
Aim of this study was the site-specific conjugation of an epidermal growth factor (EGF)-polyethylene glycol (PEG) chain by click chemistry onto a poly(amido amine) (PAMAM) dendron, as a key step toward defined multifunctional carriers for targeted gene delivery. For this purpose, at first propargyl amine cored PAMAM dendrons with ester ends were synthesized. The chain terminal ester groups were then modified by oligoamines with different secondary amino densities. The oligoamine-modified PAMAM dendrons were well biocompatible, as demonstrated in cytotoxicity assays. Among the different oligoamine-modified dendrons, PAMAM-pentaethylenehexamine (PEHA) dendron polyplexes displayed the best gene transfer ability. Conjugation of PAMAM-PEHA dendron with PEG spacer was conducted via click reaction, which was performed before amidation with PEHA. The resultant PEG-PAMAM-PEHA copolymer was then coupled with EGF ligand. pDNA transfections in HuH-7 hepatocellular carcinoma cells showed a 10-fold higher efficiency with the polyplexes containing conjugated EGF as compared to the ligand-free ones, demonstrating the concept of ligand targeting. Overall gene transfer efficiencies, however, were moderate, suggesting that additional measures for overcoming subsequent intracellular bottlenecks in delivery have to be taken
Radiative decays of decuplet hyperons
We calculate the radiative decay widths of decuplet hyperons in a chiral
constituent quark model including electromagnetic exchange currents between
quarks. Exchange currents contribute significantly to the E2 transition
amplitude, while they largely cancel for the M1 transition amplitude.
Strangeness suppression of the radiative hyperon decays is found to be weakened
by exchange currents. Differences and similarities between our results and
other recent model predictions are discussed.Comment: 11 pages, 1 eps figure, revtex, accepted for publication in Phys.
Rev.
Partially Annealed Disorder and Collapse of Like-Charged Macroions
Charged systems with partially annealed charge disorder are investigated
using field-theoretic and replica methods. Charge disorder is assumed to be
confined to macroion surfaces surrounded by a cloud of mobile neutralizing
counterions in an aqueous solvent. A general formalism is developed by assuming
that the disorder is partially annealed (with purely annealed and purely
quenched disorder included as special cases), i.e., we assume in general that
the disorder undergoes a slow dynamics relative to fast-relaxing counterions
making it possible thus to study the stationary-state properties of the system
using methods similar to those available in equilibrium statistical mechanics.
By focusing on the specific case of two planar surfaces of equal mean surface
charge and disorder variance, it is shown that partial annealing of the
quenched disorder leads to renormalization of the mean surface charge density
and thus a reduction of the inter-plate repulsion on the mean-field or
weak-coupling level. In the strong-coupling limit, charge disorder induces a
long-range attraction resulting in a continuous disorder-driven collapse
transition for the two surfaces as the disorder variance exceeds a threshold
value. Disorder annealing further enhances the attraction and, in the limit of
low screening, leads to a global attractive instability in the system.Comment: 21 pages, 2 figure
First Observation of the Doubly Charmed Baryon Xi_cc^+
We observe a signal for the doubly charmed baryon Xi_cc^+ in the charged
decay mode Xi_cc^+ --> Lambda_c^+ K- pi+ in data from SELEX, the charm
hadro-production experiment at Fermilab. We observe an excess of 15.9 events
over an expected background of 6.1 +/- 0.5 events, a statistical significance
of 6.3sigma. The observed mass of this state is (3519 +/- 1) MeV/c^2. The
Gaussian mass width of this state is 3MeV/c^2, consistent with resolution; its
lifetime is less than 33fsec at 90% confidence.Comment: 5 pages, 3 figures, accepted for publication in Physical Review
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