Scanning probe microscopy in the study of morphometric changes and physical parameters of Escherichia coli bacteria under the action of 2,4,6 - Trinitrotoluene

Using scanning probe microscopy (SPM) were registered charcteristic structural changes in Escherichia coli K12 cells under the influence of 2,4,6-trinitrotoluene (200 micrograms / ml). According to SPM data, changes were detected not only in the size of cells, but also in the structure of the cell wall. In the presence of TNT, the cell wall becomes less rough, flagellas and pils tend to be absent. However, the height of bacteria does not change. The data of force spectroscopy also showed differences in adhesion forces between the probe and the surface of the bacterial cell wall. In Escherichia coli K12 incubated with TNT there was a tendency to decrease an adhesive force compared with the control. © IDOSI Publications, 2013

Surface-dependent differences in the adsorption of pancreatic and microbial ribonucleases visualized by atomic force microscopy

A comparative study of the physical adsorption of RNAse A and RNAse Bacillus pumilis onto a negatively charged surface of mica, a hydrophobic surface of pyrolytic graphite and a surface of lipid layers of dipalmitoylphosphatidylcholine (DPPC) was performed by atomic force microscopy. It was found that microbial RNAse, unlike RNAse A, 1) is adsorbed onto the negatively charged surface of mica in the form of monomers and dimers; 2) exhibits enhanced tropism to the hydrophobic surface of pyrolytic graphite; 3) modifies morphotopography and thickness of the lipid bilayer of DPPC. The detected surface-dependent differences in adsorption of RNAses are consistent with the features of their structure and cytotoxic properties

Aplikasi Pemesanan Banten Berbasis Web dan SMS Gateway

Masyarakat Hindu di Bali merupakan umat yang memegang erat budaya, hal ini diperlihatkan dengan upacara keagamaan yang sangat sering. Seiring berkembangnya zaman,  tentunya umat Hindu di Bali tidak dapat hanya berkonsentrasi pada upacara, melainkan juga harus fokus pada karir yang sedang dijalani. Hal tersebut menjadi dilema bagi kebanyakan umat, karena mereka tidak dapat meninggalkan perkerjaan hanya untuk menyiapkan sarana upacara yang biasa disebut banten. Untuk membantu umat Hindu di Bali, maka diperlukan sebuah sistem yang yang menghubungkan antara penjual banten dengan pembeli tanpa perlu meluangkan waktu untuk bertatap muka. Dengan beberapa metode yang digunakan seperti observasi, serta metode pembuatan sistem dengan data flow diagram, diharapkan sistem yang akan dibuat dapat sesuai dengan permasalahan yang timbul. Sehingga nantinya sistem informasi SMS Gateway dan Website dapat menjadi sarana bagi pembeli untuk memesan banten tanpa harus membuang-buang waktu untuk membeli banten secara langsung

Genetic control of scald resistance in barley landraces

Scald (Rhynchosporium secalis (Oudem.) J.J. Davis pathogenic agent) is one of the most harmful barley diseases. A high variability of this fungi explains the occurrence of new aggressive pathotypes and, accordingly, loss of cultivar resistance. It was found that many recently selected varieties of barley and previously identified sources of resistance to R. secalis are now susceptible to the pathogen. There is only one gene, Rrs9, that maintains efficiency against pathogen populations in Russia. The aim of this study is to find donors of genes for effective resistance to scald with the ability to easily transfer this trait for hybridization. The inheritance of scald resistance in 33 barley landraces was studied. Analysis of the interaction between the pathogen test clones and the host plant revealed a difference between the alleles determining fungal resistance in 32 barley forms, the previously effective genes Rrs4, rrs6, rrs7, and currently effective Rrs9. It was found that 30 accessions are protected by new unidentified genes for scald resistance. Accessions k-18398 and k-16231 from China are likely to possess allelic genes for scald resistance, while the gene (or genes) of accession k-31075 from Nepal is allelic to the Rrs9 gene. It was demonstrated by hybridological analysis that accessions k-3307, k-15868, k-18989 and k-3481 are protected by effective genes for scald resistance, which differ from each other and which are not allelic to the Rrs4, rrs6, rrs7 or Rrs9 genes. Accessions k-15868 and k-3481 possess two complementary recessive genes for scald resistance, k-18989 has two recessive genes, and k-3307 carries one recessive gene for pathogen resistance. The resistance genes of these forms are manifested during all the stages of plant ontogenesis

LITHOLYTIC THERAPY AT THE TREATMENT OF CHOLELITHIASIS (REVIEW OF LITERATURE)

Treatment of cholelithiasis in patients with high operative risk need requires minimally invasive methods. Contact bilestones litholysis is a perspective direction. For this purpose it is recommended to use medications with the highest litholytic activity and the lowest side effects. It is necessary to seek for an adequate concrement dissolvent because of mineral and organic composition of bilestones. The article presents comparative analysis of characteristics of various dissolving agents proceeding from effectiveness and intensity toxic effects

DIAGNOSTICS OF HELICOBACTER PYLORI INFECTION BY ENZYME IMMUNOASSAY IN PATIENTS WITH CALCULOUS CHOLECYSTITIS

In recentyears, the interest in the significance of Helicobacter pylori in various pathological processes is growing. The most studied is the role of Helicobacter pylori in gastroduodenal pathology. Gallstone disease is one of the most common diseases of the digestive system. Inflammatory changes in the biliary tract aggravate the clinical course of gallstone disease both in the preoperative and in the postoperative period and cause strictures, deformations of the extra hepatic bile ducts in the long term. One of the most threatening complications of gallstone disease is acute cholecystitis. Determination of IgG to Helicobacter pylori in complex diagnosis is important in patients with calculous cholecystitis. We conducted a prospective study of patients (n = 105) aged 30 to 50 years, suffering from different variants of calculous cholecystitis, in the preoperative and postoperative period by enzyme immunoassay. Enzyme-linked immunosorbent assay is reliable, affordable, least invasive method for diagnosing H. pylori infection. In patients with calculous cholecystitis, we marked a significant increase in IgG antibodies to Helicobacter pylori before surgical intervention and a subsequent reduction 10 days after it. Diagnosis of Helicobacter pylori infection of the gallbladder increases therapeutic efficiency and helps to develop preventive measures for complications of gallstone disease

OPTIMIZATION OF METHODS FOR DIAGNOSTICS OF PATIENTS WITH TOXIC AND CIRRHOTIC LIVER LESIONS

Chronic liver disease is a serious problem for public health service. As a result of increasing alcoholization of population, drug addiction, number of patients with parenteral hepatitis (B, C, D) the conditions for significant increase of number of patients with hepatocirrhosis appear. The aim. of our study was to determine the most optima! methods of diagnostics of patients with toxic and cirrhotic lesions of liver. The object of the research was 67 patients treated, in Irkutsk Municipal Clinical Hospital N 1 during 2007—2011 years with liver cirrhosis. All the patients had. diagnostic laparoscopy with determination. of the level of cytokines, albumins and. gamma-globulins with further clinicomorphological and. statistical analysis of the results. It was revealed that complex use of instrumental and. laboratory methods allows to diagnose liver cirrhosis at the early stage of its progress very precisely.

Carbon nanotube-modified electrodes for electrochemical DNA-sensors

Glassy-carbon electrodes (GCEs) are modified with preoxidized multiwalled carbon nanotubes (CNTs). According to the data of atomic force microscopy, the layers of CNTs on GCEs possess a homogeneous nanostructurized surface. The voltammetric properties of a GCE/CNT depend on the modifier load. Guanine and deoxyguanosine monophosphate are strongly adsorbed on GCE/CNT and oxidized at +690 and +930 mV (pH 7.0), respectively. The oxidation current of guanine DNA nucleotides adsorbed on a GCE/CNT is significantly higher for the thermally denaturated biopolymer than for the native one. Our results are of interest for the development of sensors based on the electrochemical properties of nucleic acids. © Nauka/Interperiodica 2007

1-(8-Bromo-2-methyl-4-thioxo-3,4,5,6-tetra­hydro-2H-2,6-methano-1,3-benzoxazocin-11-yl)ethanone

In the title compound, C14H14BrNO2S, there are two similar non-equivalent mol­ecules in the asymmetric unit, displaying three chiral centres each. In the crystal structure, they are linked by inter­molecular N—H⋯O hydrogen bonds to form infinite chains, which are in turn connected by weak Br⋯H and S⋯H inter­actions

Carbon nanomaterials as electrode surface modifiers in development of amperometric monoamino oxidase biosensors

© 2016, Pleiades Publishing, Ltd.Modification of a printed graphite electrode surface by carbon nanomaterials (carbon nanotubes and graphene oxide) was used to improve the analytical capabilities of amperometric monoamine oxidase biosensors in the determination of medicinal substances with antidepressant activity (moclobemide and amitriptyline). It was found that the range of determined concentrations of pharmaceutical agents varied from 1 × 10–4 to 1 × 10–8 mol/L. The developed monoamine oxidase biosensors were used for determination of medicinal agents in their dosage forms