94 research outputs found
A hybrid fuzzy sliding-mode control for a three-phase shunt active power filter
This document is the Accepted Manuscript version of the following article: Mohamed Abdeldjabbar Kouadria, Tayeb Allaoui, and Mouloud Denai, ‘A hybrid fuzzy sliding-mode control for a three-phase shunt active power filter’, Energy Systems, Vol. 8 (2): 297-308, March 2016. The final publication is available at Springer via http://dx.doi.org/10.1007/s12667-016-0198-4.This paper describes the hybrid fuzzy sliding-mode control (HFSMC) for a three phase shunt active shunt filter for the power quality improvement. The Power Quality (PQ) problems in power distribution systems are not new but only recently the effects of these problems have gained public awareness. These non-linear loads are constructed by nonlinear devices in which the current is not proportional to the applied voltage. For the harmonic elimination different methods are used, but in this paper a novel fuzzy logic controller for a three-phase shunt active power filter for the power quality improvement such as reactive power and harmonic current compensation generated due to nonlinear loads. The hybrid fuzzy sliding-mode control (HFSMC) approach is proposed such that it can be applied with advantages to both fuzzy and sliding-mode controller. Simulation results are presented to demonstrate the effectiveness of the control strategy. The results are found to be quite satisfactory to mitigate harmonic distortions, reactive power compensation and power quality improvement.Peer reviewedFinal Accepted Versio
Effect of Boride Layer on PM HSS AISI M2 on the Mechanisms Acting in the Transverse Rupture Strength Test
Knockout of the dhfr-ts Gene in Trypanosoma cruzi Generates Attenuated Parasites Able to Confer Protection against a Virulent Challenge
Chagas disease is the clinical manifestation of the infection produced by the flagellate parasite Trypanosoma cruzi and currently there is no vaccine to prevent this disease. Therefore, different approaches or alternatives are urgently needed. Vaccination with live attenuated parasites has been used effectively in mice to reduce parasitemia and histological damage. However, the use of live parasites as inmunogens is controversial due to the risk of reversion to a virulent phenotype. In this work we genetically manipulated a naturally attenuated strain of T. cruzi in order to produce parasites with impaired replication and infectivity, using the mutation as a safety device against reversion to virulence. We show that genetically modified parasites display a lower proliferation rate in vitro and induced almost undetectable levels of T. cruzi specific CD8+ T cells when injected in mice. Furthermore, the immune response induced by these live mutant parasites confers protection against a subsequent virulent infection even a year after the original immunization
HpaC Controls Substrate Specificity of the Xanthomonas Type III Secretion System
The Gram-negative bacterial plant pathogen Xanthomonas campestris pv. vesicatoria employs a type III secretion (T3S) system to inject bacterial effector proteins into the host cell cytoplasm. One essential pathogenicity factor is HrpB2, which is secreted by the T3S system. We show that secretion of HrpB2 is suppressed by HpaC, which was previously identified as a T3S control protein. Since HpaC promotes secretion of translocon and effector proteins but inhibits secretion of HrpB2, HpaC presumably acts as a T3S substrate specificity switch protein. Protein–protein interaction studies revealed that HpaC interacts with HrpB2 and the C-terminal domain of HrcU, a conserved inner membrane component of the T3S system. However, no interaction was observed between HpaC and the full-length HrcU protein. Analysis of HpaC deletion derivatives revealed that the binding site for the C-terminal domain of HrcU is essential for HpaC function. This suggests that HpaC binding to the HrcU C terminus is key for the control of T3S. The C terminus of HrcU also provides a binding site for HrpB2; however, no interaction was observed with other T3S substrates including pilus, translocon and effector proteins. This is in contrast to HrcU homologs from animal pathogenic bacteria suggesting evolution of distinct mechanisms in plant and animal pathogenic bacteria for T3S substrate recognition
Structure-Function Analysis of the HrpB2-HrcU Interaction in the Xanthomonas citri Type III Secretion System
Bacterial type III secretion systems deliver protein virulence factors to host cells. Here we characterize the interaction between HrpB2, a small protein secreted by the Xanthomonas citri subsp. citri type III secretion system, and the cytosolic domain of the inner membrane protein HrcU, a paralog of the flagellar protein FlhB. We show that a recombinant fragment corresponding to the C-terminal cytosolic domain of HrcU produced in E. coli suffers cleavage within a conserved Asn264-Pro265-Thr266-His267 (NPTH) sequence. A recombinant HrcU cytosolic domain with N264A, P265A, T266A mutations at the cleavage site (HrcUAAAH) was not cleaved and interacted with HrpB2. Furthermore, a polypeptide corresponding to the sequence following the NPTH cleavage site also interacted with HrpB2 indicating that the site for interaction is located after the NPTH site. Non-polar deletion mutants of the hrcU and hrpB2 genes resulted in a total loss of pathogenicity in susceptible citrus plants and disease symptoms could be recovered by expression of HrpB2 and HrcU from extrachromossomal plasmids. Complementation of the ΔhrcU mutant with HrcUAAAH produced canker lesions similar to those observed when complemented with wild-type HrcU. HrpB2 secretion however, was significantly reduced in the ΔhrcU mutant complemented with HrcUAAAH, suggesting that an intact and cleavable NPTH site in HrcU is necessary for total functionally of T3SS in X. citri subsp. citri. Complementation of the ΔhrpB2 X. citri subsp. citri strain with a series of hrpB2 gene mutants revealed that the highly conserved HrpB2 C-terminus is essential for T3SS-dependent development of citrus canker symptoms in planta
Effect of Diffusion Annealing on Borides Layers Produced on XC38 Steel
In this work, we present a study of the effect of diffusion annealing at 700°C for 1 h on the nature and properties of boride layers obtained on XC38 steel through a molten salt consisting of borax (Na₂B₄O₇) and boron carbide (B₄C). We evaluated the changes brought by the diffusion annealing on the morphology of the boride layer, the thickness of this layer, the distribution of elements in the steel, and the hardness. Comparing the results obtained allowed concluding that the diffusion annealing will completely transform the two-phase layer formed of FeB and Fe₂B borides in a single-phase layer consisting of single boride Fe₂B. The transformation of the two-phase boride into a single-phase boride is done with an increase in thickness of about 30% compared to the initial thickness of the sample. The values of Si concentration obtained in the underlying zone after the diffusion annealing treatment are more important than those obtained in the same underlying zone of samples borided directly by immersion in molten salt consisting of borax and silicon carbide (SiC)
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