Morphogenic microspore as an initial cell of androgenesis in vitro: review of the problem

The phenomenon of androgenesis in vitro is widely used in biotechnological investigations of commercially valuable plants. When solving the specific tasks it is important to enter into the culture in vitro anthers containing sporogenous cells which are morphogenetic competent to change the development program from gametophytic to sporophytic on

Phytohormonal regulation of in vitro formation of wheat androgenic structures

This research is devoted to developing a method of phytohormonal regulation of in vitro formation of a certain type of wheat androgenic structures. Using the method of ELISA it was shown that the induction of certain sporophytic morphogenesis pathway in vitro of anther haploid cells - microspores depends on both the content of endogenous auxin IAA in anthers before inoculating them onto induction medium, and the concentration of exogenous auxin 2,4-D in this medium. The obtained data confirms the principle possibility of regulation of ways of getting androgenic regenerants in vitro by selecting the optimal balance of endogenous and exogenous auxin

Morphogenic microspore as an initial cell of androgenesis in vitro: review of the problem

The phenomenon of androgenesis in vitro is widely used in biotechnological investigations of commercially valuable plants. When solving the specific tasks it is important to enter into the culture in vitro anthers containing sporogenous cells which are morphogenetic competent to change the development program from gametophytic to sporophytic on

Phytohormonal regulation of in vitro formation of wheat androgenic structures

This research is devoted to developing a method of phytohormonal regulation of in vitro formation of a certain type of wheat androgenic structures. Using the method of ELISA it was shown that the induction of certain sporophytic morphogenesis pathway in vitro of anther haploid cells - microspores depends on both the content of endogenous auxin IAA in anthers before inoculating them onto induction medium, and the concentration of exogenous auxin 2,4-D in this medium. The obtained data confirms the principle possibility of regulation of ways of getting androgenic regenerants in vitro by selecting the optimal balance of endogenous and exogenous auxin