The effect of water dynamics on conformation changes of albumin in pre-denaturation state:photon correlation spectroscopy and simulation

Water is essential for protein three-dimensional structure, conformational dynamics, and activity. Human serum albumin (HSA) is one of major blood plasma proteins, and its functioning is fundamentally determined by the dynamics of surrounding water. The goal of this study is to link the conformational dynamics of albumin to the thermal motions in water taking place in the physiological temperature range. We report the results of photon correlation spectroscopy and molecular dynamics simulations of HSA in aqueous solution. The experimental data processing produced the temperature dependence of the HSA hydrodynamic radius and its zeta potential. Molecular dynamics reproduced the results of experiments and revealed changes in the secondary structure caused by the destruction of hydrogen bonds in the macromolecule's globule

Networked buffering: a basic mechanism for distributed robustness in complex adaptive systems

A generic mechanism - networked buffering - is proposed for the generation of robust traits in complex systems. It requires two basic conditions to be satisfied: 1) agents are versatile enough to perform more than one single functional role within a system and 2) agents are degenerate, i.e. there exists partial overlap in the functional capabilities of agents. Given these prerequisites, degenerate systems can readily produce a distributed systemic response to local perturbations. Reciprocally, excess resources related to a single function can indirectly support multiple unrelated functions within a degenerate system. In models of genome:proteome mappings for which localized decision-making and modularity of genetic functions are assumed, we verify that such distributed compensatory effects cause enhanced robustness of system traits. The conditions needed for networked buffering to occur are neither demanding nor rare, supporting the conjecture that degeneracy may fundamentally underpin distributed robustness within several biotic and abiotic systems. For instance, networked buffering offers new insights into systems engineering and planning activities that occur under high uncertainty. It may also help explain recent developments in understanding the origins of resilience within complex ecosystems. \ud \u

Quasielastic scattering of slow-neutron in water-alcohol solutions

Research of molecules dynamics of solutions “water - propyl alcohol” of different concentration at the temperature 281 K is conducted by the method of slow-neutron quasi-elastic scattering. There were experimentally exposed the feature of effective self-diffusion coefficient of molecules of the indicated solutions. Based on the time- scale hierarchy the division of selfdiffusion coefficient to one-particle and collective contributions was conducted, and the time of the molecules settled life in position of equilibrium was calculated. There were also exposed the feature of self-diffusion concentration dependence of coefficient of self-diffusion and his selfpart contribution, namely: presence of two minimums is in the areas of concentrations (0,04 ÷ 0,05) of mass fraction and (0,18 ÷ 0,22) m.c. of the alcohol and continuous character of diffusion at concentrations higher then 0,4 m.c. of the alcohol. It is shown that the indicated concentration areas correspond the certain local structures of investigational solution

Retrospective analysis of junior female handball players’ priorities

Purpose: fulfillment of retrospective analysis of junior female handball players’ tactic priorities. Material: in the research junior female handball players of 15-16 yrs age (n=60) participated. The researches were conducted in 2006, 2010 and 2016 on the base of sport schools and physical culture colleges of Ukraine. We used author’s programs «Balltest» and «Handball skills». Results: indicators of junior female handball players’ abilities and tactical thinking effectiveness in different periods of the research were received. Correlations of these indicators with physical potentials and throw fitness point at tactical priorities of the players. Comparative characteristic showed that junior female handball players of 2016 year of the research had better abilities for solution of complex team tasks with low sensor indicators. We found handball players’ preferences to defensive and attacking actions in central zone of site. Conclusions: by universal character of tactic priorities junior female handball players of 2016 year of the research yield to the players of 2006 and 2010 years of the research. Junior female handball players of 2016 year of the research prevail in successful mental solution of position defense tactic tasks, especially in readiness to act as supporters