The study of the regulatory region of the Drosophila melanogaster Notch gene by new methods of directed genome editing

The Notch gene plays a key role in the development of organs and tissues of neuroectodermic origin, including the nervous system. In eukaryotic organisms, the Notch pathway is involved in cell fate determination. The Notch gene was first discovered in Drosophila melanogaster. In mammals, the family of Notch receptors includes four homologues. In humans, mutations in the Notch gene cause several hereditary diseases and carcinogenesis. Studies of the regulatory zone of the Notch gene in D. melanogaster have been conducted for several decades. We review their results and methods. The regulatory zone of the Notch gene is in the region of open chromatin state that corresponds to the 3C6/3C7 interband on the cytological map of polytene chromosomes of D. melanogaster salivary glands. The development of new methods for directed genome editing made it possible to create a system for introducing directed changes into the regulatory zone of the gene. Using the CRISPR/Cas9 system, we obtained a directed 4-kilobase deletion including the 5’-regulatory zone, promoter, and the first exon of the Notch gene and introduced the attP site into the first intron of the Notch gene. This approach enabled targeted changes of the sequence of the regulatory and promoter regions of the gene. Thus, it provided a new powerful tool for studies of Notch gene regulation and the organization of the open chromatin state

Новые данные о распространении домового сыча в Новосибирской области и северной части Алтайского края, Россия

В сообщении приводятся новые данные о встречах домового сыча (Athene noctua) в Искитимском районе Новосибирской области и Крутихинском районе Алтайского края. Уточняется информация о находках домового сыча в Карасукском районе Новосибирской области

Pharyngomycosis. Diagnostics, prevention and treatment

The prevalence of pharyngomycosis was analysed on the basis of research data and own observation of 2,987 patients seeking assistance in the consulting unit of the Centre over the period from 2008 till 2011. The study concluded that the proportion of fungal infections of ENT organs in the total variety of chronic inflammatory disease increased to 29% for fungal infections of the throat in pharyngitis, and to 17.2% in chronic tonsillitis. The pathogens of fungal infections of the throat were defined and analyzed. The study found that the involvement of Candida non albicans strains in the development of chronic inflammation has increased. The proportion of cases of aspergillus infection of the mucous membranes of the pharynx, larynx, and tonsils was identified. Sophisticated diagnosis and treatment schemes were suggested

Northern Hawk Owl invasions in the forest-steppe of Western Siberia

The article presents data on the invasions of the hawk owl (Surnia ulula) into the forest-steppe of Western Siberia on the territory of the Novosibirsk and Kemerovo regions, Altai Krai and Krasnoyarsk Krai territories