The Concept of Ecologically Oriented Progress and Natural Resource Preservation

The most important issue of scientific and technological progress is considering the environment challenges of industrial development. It means that the progress must be ecologically oriented and environmentally friendly. The most adequate concept for the approach to the issue of "man - society – nature" relations is the ontology of the noosphere - the idea of a common space for human beings and nature. It presents an ideal example of an optimistic attitude towards the coordination between accelerating the scientific and technological development and natural resource saving. However, to maintain the balance between human needs and environmental processes determined by this concept, it is essential to include the lean production training into technological development of society

Status epilepticus: Analysis of refractory cases

Objective: to analyze refractory status epilepticus (SE) cases.Patients and methods. Fifteen female patients aged 21 to 62 years with refractory SE were comprehensively examined using long-term electroencephalography monitoring. The investigators evaluated the efficiency of treatment regimens with intravenous antiepileptic drugs (AEDs), such as diazepam (DZP); valproic acid (VPA); levetiracetam; and lacosamide and their combinations, at the prehospital and hospital stages, as well as SE therapy complications noted only in the intravenous administration of narcotics (propofol, sodium thiopental).Results and discussion. A fetal outcome due to multiple organ dysfunction indirectly related to SE was recorded in 2 (13.3%) patients with acute symptomatic status. SE was abolished in the other 13 cases. The preliminary findings may suggest that it is appropriate to prescribe VPA just at the prehospital stage. The co-administration of VPA and DZP substantially enhances the efficiency of SE therapy. The maximum acceptable doses of AEDs using the whole available therapeutic arsenal should be administered within the first hours of acute symptomatic SE

Tandemly repeated DNA families in the mouse genome

<p>Abstract</p> <p>Background</p> <p>Functional and morphological studies of tandem DNA repeats, that combine high portion of most genomes, are mostly limited due to the incomplete characterization of these genome elements. We report here a genome wide analysis of the large tandem repeats (TR) found in the mouse genome assemblies.</p> <p>Results</p> <p>Using a bioinformatics approach, we identified large TR with array size more than 3 kb in two mouse whole genome shotgun (WGS) assemblies. Large TR were classified based on sequence similarity, chromosome position, monomer length, array variability, and GC content; we identified four superfamilies, eight families, and 62 subfamilies - including 60 not previously described. 1) The superfamily of centromeric minor satellite is only found in the unassembled part of the reference genome. 2) The pericentromeric major satellite is the most abundant superfamily and reveals high order repeat structure. 3) Transposable elements related superfamily contains two families. 4) The superfamily of heterogeneous tandem repeats includes four families. One family is found only in the WGS, while two families represent tandem repeats with either single or multi locus location. Despite multi locus location, TRPC-21A-MM is placed into a separated family due to its abundance, strictly pericentromeric location, and resemblance to big human satellites.</p> <p>To confirm our data, we next performed <it>in situ </it>hybridization with three repeats from distinct families. TRPC-21A-MM probe hybridized to chromosomes 3 and 17, multi locus TR-22A-MM probe hybridized to ten chromosomes, and single locus TR-54B-MM probe hybridized with the long loops that emerge from chromosome ends. In addition to <it>in silico </it>predicted several extra-chromosomes were positive for TR by <it>in situ </it>analysis, potentially indicating inaccurate genome assembly of the heterochromatic genome regions.</p> <p>Conclusions</p> <p>Chromosome-specific TR had been predicted for mouse but no reliable cytogenetic probes were available before. We report new analysis that identified <it>in silico </it>and confirmed <it>in situ </it>3/17 chromosome-specific probe TRPC-21-MM. Thus, the new classification had proven to be useful tool for continuation of genome study, while annotated TR can be the valuable source of cytogenetic probes for chromosome recognition.</p

ИССЛЕДОВАНИЕ СТЕКЛОПЛАСТИКОВЫХ ТРУБ С РАЗНОЙ ТРАНСВЕРСАЛЬНО-ИЗОТРОПНОЙ СТРУКТУРОЙ ДЛЯ РАЗНЫХ УСЛОВИЙ ЭКСПЛУАТАЦИИ

Виявлено складнощі у проектуванні склопластикових труб з анізотропною структурою, які використовують у системах лісового, міського та сільського хазяйств у зв'язку з різними умовами навантаження та розподілу навантаження у повздовжньому та поперечному (кільцевому) напрямах. Зумовлено вибір отримання склопластикових труб методом косошарого поздовжньо-поперечного намотування, як найперспективнішого з можливістю контролювання трансверсально-ізотропної структури склопластикових виробів круглого перетину. Проаналізовано кількість наповнювача, який укладається у повздовжньому напрямі, та кількість наповнювача, який укладається у поперечному напрямку, та їх співвідношення залежно від трансверсально-ізотропної структури склопластикових труб. За результатами експериментального дослідження наведено дані про фізико-механічні показники, а саме руйнівну напругу у повздовжньому та поперечному напрямах, склопластикових виробів та їх залежність від трансверсально-ізотропної структури, а саме від кількості та напряму, в якому вкладається армувальний матеріал. Встановлено можливість регулювання фізико-механічних властивостей у повздовжньому та поперечному напрямах, їх комбінації відповідно до навантаження за різних умов експлуатації.Glass-plastic pipes are widely used in the field of forestry, municipal services and agriculture, in irrigation and melioration systems, collector-drainage networks, technological and main pipelines of fire protection systems and water supply systems, for transportation of chemical aggressive environments and oil products, for gas pipelines and sewage systems, water-raising columns and others. A significant number of operating conditions involves the utilization of fiberglass pipes with different physical and mechanical characteristics. The method of cross-fibred longitudinal-circumferential winding allows receiving products with a different transversal-isotropic structure. The study has established the influence of this structure on the physical and mechanical properties of fiberglass pipes – the failure stress at tension in the longitudinal and transverse directions. The authors have indicated the dependence of the amount of the filler in the transversal and axial directions, their ratio on the transversally isotropic structure. Based on the results of study, the authors have found the dependence of the failure stress at tension in the longitudinal and transverse directions on the amount and ratio of the filler in the transversal and axial directions. The obtained data permits predicting and simulating construction fiberglass plastics with the necessary characteristics of the failure stress at tension in the longitudinal and transverse directions, or their combinations by changing the transversal-isotropic structure for each individual pipeline with certain operating conditions. The adjustment of the combination of physical and mechanical properties in longitudinal and transverse directions makes it possible to choose a wall thickness of the product that would correspond to the minimum permissible value in both directions, and not only in one of them. A further study of the dependence of the failure compression and bending stress and their elastic modulus will allow assuming that all physical and mechanical properties have a similar dependence on the transversally isotropic system and bring new data for more detailed design and manufacture of pipelines with different loading conditions.Установлены сложности при проектировании стеклопластиковых труб с анизотропной структурой, которые используются в системах лесного, городского и сельского хозяйств, в связи с разными условиями и распределения нагрузки в продольном и поперечном (кольцевом) направлениях. Обусловлен выбор производства стеклопластиковых труб методом косослойной продольно-поперечной намотки, как самого перспективного с возможностью контролирования трансверсально-изотропной структуры стеклопластиковых изделий круглого сечения. Проанализировано количество наполнителя, уложенного в продольном направлении, и количество наполнителя уложенного в поперечном направлении, и их соотношение в зависимости от трансверсально-изотропной структуры стеклопластиковых труб. По результатам экспериментальных исследований приведены данные о физико-механических показателях, а именно разрушающем напряжении в продольном и поперечном направлениях, стеклопластиковых изделий и их зависимость от трансверсально-изотропной структуры, а именно от количества и направления, в котором уложен армирующий материал. Установлена возможность регулирования физико-механических свойств в продольном и поперечном направлениях, их комбинацию соответственно к нагрузке при разных условиях эксплуатации

Development and characterization of polyclonal antibodies against the linker region of the telomere-binding protein TRF2

Background: TRF2 (telomeric repeat binding factor 2) is an essential component of the telomere-binding protein complex shelterin. TRF2 induces the formation of a special structure of telomeric DNA and counteracts activation of DNA damage-response pathways telomeres. TRF2 has a poorly characterized linker region (udTRF2) between its homodimerization and DNA-binding domains. Some lines of evidence have shown that this region could be involved in TRF2 interaction with nuclear lamina. Results: In this study, the fragment of the TERF2 gene encoding udTRF2 domain of telomere-binding protein TRF2 was produced by PCR and cloned into the pET32a vector. The resulting plasmid pET32a-udTRF2 was used for the expression of the recombinant udTRF2 in E. coli RosettaBlue (DE3). The protein was isolated and purified using ammonium sulfate precipitation followed by ion-exchange chromatography. The purified recombinant protein udTRF2 was injected into guinea pigs to generate polyclonal antibodies. The ability of anti-udTRF2 antibodies to bind endogenous TRF2 in human skin fibroblasts was tested by western blotting and immunofluorescent staining. Conclusions: In this study, the recombinant protein udTRF2 and antibodies to it were generated. Both protein and antibodies will provide a useful tool for investigation of the functions of the udTRF2 domain and its role in the interaction between TRF2 and nuclear lamina. Keywords: Chromosomes, Molecular cloning, Nuclear lamina, Nucleoprotein complexes, Polyclonal antibodies, Recombinant polypeptide, Shelterin, Telomere-binding protein TRF2, Telomeres, Telomeric DNA, TTAGGG repeat

Human chromosome 1 satellite 3 DNA is decondensed, demethylated and transcribed in senescent cells and in A431 epithelial carcinoma cells

Constitutive heterochromatin mainly consists of different classes of satellite DNAs and is defined as a transcriptionally inactive chromatin that remains compact throughout the cell cycle. The aim of this work was to investigate the level of condensation, methylation and transcriptional status of centromeric (alphoid DNA) and pericentromeric satellites (human satellite 3, HS3) in tissues (lymphocytes, placenta cells) and in cultured primary (MRC5, VH-10, AT2Sp) and malignant (A431) cells. We found that alphoid DNA remained condensed and heavily methylated in all the cell types. The HS3 of chromosome 1 (HS3-1) but not of chromosome 9 (HS3-9) was strongly decondensed and demethylated in A431 cells. The same observation was made for aged embryonic lung (MRC5) and juvenile foreskin (VH-10) fibroblasts obtained at late passages (32nd and 23rd, respectively). Decondensation was also found in ataxia telangiectasia AT2Sp fibroblasts at the 16th passage. One of the manifestations of the disease is premature aging. The level of HS3-1 decondensation was higher in aged primary fibroblasts as compared to A431. The HS3-1 extended into the territory of neighbouring chromosomes. An RT-PCR product was detected in A431 and senescent MRC5 fibroblasts using primers specific for HS3-1. The RNA was polyadenylated and transcribed from the reverse chain. Our results demonstrate the involvement of satellite DNA in associations between human chromosomes and intermingling of chromosome territories. The invading satellite DNA can undergo decondensation to a certain level. This process is accompanied by demethylation and transcription