7,392 research outputs found
Differentiation and Regenerative Capacities of Human Odontoma-Derived Mesenchymal Cells
Regenerating human tooth ex vivo and biological repair of dental caries are hampered by non-viable odontogenic stem cells that can regenerate different tooth components. Odontoma is a developmental dental anomaly that may contain putative post-natal stem cells with the ability to differentiate and regenerate in vivo new dental structures that may include enamel, dentin, cementum and pulp tissues. We evaluated odontoma tissues from 14 patients and further isolated and characterized human odontoma-derived mesenchymal cells (HODCs) with neural stem cell and hard tissue regenerative properties from a group of complex odontoma from 1 of 14 patients. Complex odontoma was more common (9 of 14) than compound type and females (9 of 14) were more affected than males in our set of patients. HODCs were highly proliferative like dental pulp stem cells (DPSCs) but demonstrated stronger neural immunophenotype than both DPSCs and mandible bone marrow stromal cells (BMSCs) by expressing higher levels of nestin, Sox 2 and βIII-tubulin. When transplanted with hydroxyapatite/tricalcium phosphate into immunocompromised mice, HODCs differentiated and regenerated calcified hard tissues in vivo that were morphologically and quantitatively comparable to those generated by DPSCs and BMSCs. When transplanted with polycaprolactone (biodegradable carrier), HODCs differentiated to form new predentin on the surface of a dentin platform. Newly formed predentin contained numerous distinct dentinal tubules and an apparent dentin-pulp arrangement. HODCs represent unique odontogenic progenitors that readily commit to formation of dental hard tissues
SU(2) symmetry in a Hubbard model with spin-orbit coupling
We study the underlying symmetry in a spin-orbit coupled tight-binding model
with Hubbard interaction. It is shown that, in the absence of the on-site
interaction, the system possesses the SU(2) symmetry arising from the
timereversal symmetry. The influence of the on-site interaction on the symmetry
depends on the topology of the networks: The SU(2) symmetry is shown to be the
spin rotation symmetry of a simply-connected lattice, so it still holds in the
presence of the Hubbard correlation. In contrary, the on-site interaction
breaks the SU(2) symmetry of a multi-connected lattice.Comment: 5 pages, 2 figure
A Novel Meatoplasty Method in Canal Wall Down Tympanomastoidectomy: A Perichondrial Posterior Fixation Technique
ObjectivesAlthough it is well recognized that a small meatus after canal wall down (CWD) tympanomastoidectomy can cause a lifetime problem, unsatisfactory results are frequently encountered. We herein introduce a novel technique, perichondrial posterior fixation (PPF), to maintain a wide external auditory canal (EAC), to minimize postoperative wound infection due to the smaller dead space and to improve the posterior auricular cosmetic outcome.MethodsA total of 73 patients who underwent CWD tympanomastoidectomy were included. Interventions are CWD tympanomastoidectomy with the PPF technique. Review of the medical records and evaluation of the postoperative size of the meatus and the extent of the cavum conchal cartilage buried within the mastoid cavity by taking digital photographs.ResultsThirty males and 43 females were included and the mean age was 44.1 yr (range, 6 to 66 yr). The mean follow-up duration was 26 months (range, 12 to 56 months). All ears maintained a clean and large external meatus. The posterior auricular cavum conchal cartilage was successfully prevented from being buried into the mastoid cavity in all ears. The extent of cartilage buried into the mastoid cavity was much reduced compared to the conventional technique.ConclusionThe PPF technique, which is a novel meatoplasty technique in CWD tympanomastoidectomy, seems to be effective in maintaining a large external meatus and improving the cosmetic outcome with minimal risk of complications
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killerFLIP: a novel lytic peptide specifically inducing cancer cell death
One of the objectives in the development of effective cancer therapy is induction of tumor-selective cell death. Toward this end, we have identified a small peptide that, when introduced into cells via a TAT cell-delivery system, shows a remarkably potent cytoxicity in a variety of cancer cell lines and inhibits tumor growth in vivo, whereas sparing normal cells and tissues. This fusion peptide was named killerFLIP as its sequence was derived from the C-terminal domain of c-FLIP, an anti-apoptotic protein. Using structure activity analysis, we determined the minimal bioactive core of killerFLIP, namely killerFLIP-E. Structural analysis of cells using electron microscopy demonstrated that killerFLIP-E triggers cell death accompanied by rapid (within minutes) plasma membrane permeabilization. Studies of the structure of the active core of killerFLIP (-E) indicated that it possesses amphiphilic properties and self-assembles into micellar structures in aqueous solution. The biochemical properties of killerFLIP are comparable to those of cationic lytic peptides, which participate in defense against pathogens and have also demonstrated anticancer properties. We show that the pro-cell death effects of killerFLIP are independent of its sequence similarity with c-FLIPL as killerFLIP-induced cell death was largely apoptosis and necroptosis independent. A killerFLIP-E variant containing a scrambled c-FLIPL motif indeed induced similar cell death, suggesting the importance of the c-FLIPL residues but not of their sequence. Thus, we report the discovery of a promising synthetic peptide with novel anticancer activity in vitro and in vivo
Interaction between TATA-Binding Protein (TBP) and Multiprotein Bridging Factor-1 (MBF1) from the Filamentous Insect Pathogenic Fungus Beauveria bassiana
TATA-binding protein (TBP) is a ubiquitous component of eukaryotic transcription factors that acts to nucleate assembly and position pre-initiation complexes. Multiprotein bridging factor 1 (MBF1) is thought to interconnect TBP with gene specific transcriptional activators, modulating transcriptional networks in response to specific signal and developmental programs. The insect pathogen, Beauveria bassiana, is a cosmopolitan fungus found in most ecosystems where it acts as an important regulator of insect populations and can form intimate associations with certain plants. In order to gain a better understanding of the function of MBF1 in filamentous fungi, its interaction with TBP was demonstrated. The MBF1 and TBP homologs in B. bassiana were cloned and purified from a heterologous E. coli expression system. Whereas purified BbTBP was shown to be able to bind oligonucleotide sequences containing the TATA-motif (Kd ≈ 1.3 nM) including sequences derived from the promoters of the B. bassiana chitinase and protease genes. In contrast, BbMBF1 was unable to bind to these same target sequences. However, the formation of a ternary complex between BbMBF1, BbTBP, and a TATA-containing target DNA sequence was seen in agarose gel electrophoretic mobility shift assays (EMSA). These data indicate that BbMBF1 forms direct interactions with BbTBP, and that the complex is capable of binding to DNA sequences containing TATA-motifs, confirming that BbTBP can link BbMBF1 to target sequences as part of the RNA transcriptional machinery in fungi
The hadron-quark phase transition in dense matter and neutron stars
We study the hadron-quark phase transition in the interior of neutron stars
(NS's). We calculate the equation of state (EOS) of hadronic matter using the
Brueckner-Bethe-Goldstone formalism with realistic two-body and three-body
forces, as well as a relativistic mean field model. For quark matter we employ
the MIT bag model constraining the bag constant by using the indications coming
from the recent experimental results obtained at the CERN SPS on the formation
of a quark-gluon plasma. We find necessary to introduce a density dependent bag
parameter, and the corresponding consistent thermodynamical formalism. We
calculate the structure of NS interiors with the EOS comprising both phases,
and we find that the NS maximum masses fall in a relatively narrow interval,
. The precise value of the
maximum mass turns out to be only weakly correlated with the value of the
energy density at the assumed transition point in nearly symmetric nuclear
matter.Comment: 25 pages, Revtex4, 16 figures included as postscrip
Rms-flux relation of Cyg X-1 with RXTE: dipping and nondipping cases
The rms (root mean square) variability is the parameter for understanding the
emission temporal properties of X-ray binaries (XRBs) and active galactic
nuclei (AGN).
The rms-flux relation with Rossi X-ray Timing Explorer (RXTE) data for the
dips and nondip of black hole Cyg X-1 has been investigated in this paper. Our
results show that there exist the linear rms-flux relations in the frequency
range 0.1-10 Hz for the dipping light curve. Moreover, this linear relation
still remains during the nondip regime, but with the steeper slope than that of
the dipping case in the low energy band. For the high energy band, the slopes
of the dipping and nondipping cases are hardly constant within errors. The
explanations of the results have been made by means of the ``Propagating
Perturbation'' model of Lyubarskii (1997).Comment: 15 pages, 12 figures, Accepted for publication in Astrophysics &
Space Scienc
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