Varroa mite evolution: a neglected aspect of worldwide bee collapses?

While ectoparasitic Varroa mites cause minimal damage to their co-evolved ancestral host, the eastern honey bee (Apis cerana), they devastate their novel host, the western honey bee (Apis mellifera). Over several decades, the host switch caused worldwide population collapses, threatening global food security. Varroa management strategies have focused on breeding bees for tolerance. But, can Varroa overcome these counter-adaptations in a classic coevolutionary arms race? Despite increasing evidence for Varroa genetic diversity and evolvability, this eventuality has largely been neglected. We therefore suggest a more holistic paradigm for studying this host-parasite interaction, one in which \u27Varroa-tolerant\u27 bee traits should be viewed as a shared phenotype resulting from Varroa and honey bee interaction

Vector-virus interaction affects viral loads and co-occurrence

BackgroundVector-borne viral diseases threaten human and wildlife worldwide. Vectors are often viewed as a passive syringe injecting the virus. However, to survive, replicate and spread, viruses must manipulate vector biology. While most vector-borne viral research focuses on vectors transmitting a single virus, in reality, vectors often carry diverse viruses. Yet how viruses affect the vectors remains poorly understood. Here, we focused on the varroa mite (Varroa destructor), an emergent parasite that can carry over 20 honey bee viruses, and has been responsible for colony collapses worldwide, as well as changes in global viral populations. Co-evolution of the varroa and the viral community makes it possible to investigate whether viruses affect vector gene expression and whether these interactions affect viral epidemiology.ResultsUsing a large set of available varroa transcriptomes, we identified how abundances of individual viruses affect the vector’s transcriptional network. We found no evidence of competition between viruses, but rather that some virus abundances are positively correlated. Furthermore, viruses that are found together interact with the vector’s gene co-expression modules in similar ways, suggesting that interactions with the vector affect viral epidemiology. We experimentally validated this observation by silencing candidate genes using RNAi and found that the reduction in varroa gene expression was accompanied by a change in viral load.ConclusionsCombined, the meta-transcriptomic analysis and experimental results shed light on the mechanism by which viruses interact with each other and with their vector to shape the disease course

Evolutionarily diverse origins of deformed wing viruses in western honey bees

Novel transmission routes can allow infectious diseases to spread, often with devastating consequences. Ectoparasitic varroa mites vector a diversity of RNA viruses, having switched hosts from the eastern to western honey bees (Apis cerana to Apis mellifera). They provide an opportunity to explore how novel transmission routes shape disease epidemiology. As the principal driver of the spread of deformed wing viruses (mainly DWV-A and DWV-B), varroa infestation has also driven global honey bee health declines. The more virulent DWV-B strain has been replacing the original DWV-A strain in many regions over the past two decades. Yet, how these viruses originated and spread remains poorly understood. Here, we use a phylogeographic analysis based on whole-genome data to reconstruct the origins and demography of DWV spread. We found that, rather than reemerging in western honey bees after varroa switched hosts, as suggested by previous work, DWV-A most likely originated in East Asia and spread in the mid-20th century. It also showed a massive population size expansion following the varroa host switch. By contrast, DWV-B was most likely acquired more recently from a source outside East Asia and appears absent from the original varroa host. These results highlight the dynamic nature of viral adaptation, whereby a vector's host switch can give rise to competing and increasingly virulent disease pandemics. The evolutionary novelty and rapid global spread of these host-virus interactions, together with observed spillover into other species, illustrate how increasing globalization poses urgent threats to biodiversity and food security

Evolutionarily diverse origins of deformed wing viruses in western honey bees

Novel transmission routes can allow infectious diseases to spread, often with devastating consequences. Ectoparasitic varroa mites vector a diversity of RNA viruses, having switched hosts from the eastern to western honey bees (Apis cerana to Apis mellifera). They provide an opportunity to explore how novel transmission routes shape disease epidemiology. As the principal driver of the spread of deformed wing viruses (mainly DWV-A and DWV-B), varroa infestation has also driven global honey bee health declines. The more virulent DWV-B strain has been replacing the original DWV-A strain in many regions over the past two decades. Yet, how these viruses originated and spread remains poorly understood. Here, we use a phylogeographic analysis based on whole-genome data to reconstruct the origins and demography of DWV spread. We found that, rather than reemerging in western honey bees after varroa switched hosts, as suggested by previous work, DWV-A most likely originated in East Asia and spread in the mid-20th century. It also showed a massive population size expansion following the varroa host switch. By contrast, DWV-B was most likely acquired more recently from a source outside East Asia and appears absent from the original varroa host. These results highlight the dynamic nature of viral adaptation, whereby a vector's host switch can give rise to competing and increasingly virulent disease pandemics. The evolutionary novelty and rapid global spread of these host-virus interactions, together with observed spillover into other species, illustrate how increasing globalization poses urgent threats to biodiversity and food security

Varroa mite evolution: a neglected aspect of worldwide bee collapses?

While ectoparasitic Varroa mites cause minimal damage to their co-evolved ancestral host, the eastern honey bee (Apis cerana), they devastate their novel host, the western honey bee (Apis mellifera). Over several decades, the host switch caused worldwide population collapses, threatening global food security. Varroa management strategies have focused on breeding bees for tolerance. But, can Varroa overcome these counter-adaptations in a classic coevolutionary arms race? Despite increasing evidence for Varroa genetic diversity and evolvability, this eventuality has largely been neglected. We therefore suggest a more holistic paradigm for studying this host-parasite interaction, one in which ‘Varroa-tolerant’ bee traits should be viewed as a shared phenotype resulting from Varroa and honey bee interactionASM was supported by a Future Fellowship from the Australian Research Council (FT160100178

The effect of DEET on chemosensing of the honey bee and its parasite Varroa destructor

International audienceVarroa destructor Anderson and Trueman (Acari: Varroidae) is an obligatory ectoparasitic mite of honey bees. In view of limited success in mite control, the use of synthetic repellent was evaluated. The objective of the present study was to investigate the effect of common arthropod repellent N,N-diethyl-m-toluamide (DEET) on the chemosensing of the V. destructor and its hosts, the European honey bee (Apis mellifera L.), by electrophysiological and behavioural bioassays. In electrophysiological assays, the nurse headspace served as a positive stimulus for the V. destructor foreleg, whereas a queen headspace was used as a positive stimulus for honey bee antennae. Two effects of DEET on V. destructor host chemosensing were evaluated: short-term inhibition and long-term inhibition. The inhibition observed in the presence of DEET simultaneously with a positive stimulus was termed “short term inhibition”, while inhibition that occurred following the administration of the compound alone was termed “long term inhibition”. In V. destructor, DEET served as a long-term inhibitor to the response of the chemosensory organ to nurse bee headspace volatiles, whereas in honey bee, it caused short-term inhibition of antenna response to queen volatiles. Consistent with electrophysiological studies, DEET significantly inhibited host choice of mites, whereas even a 10 times higher dose did not alter honey bee behaviours (e.g. antennating, grooming, fanning etc.) or worker attraction to a queen. These data suggest that DEET may selectively disrupt the honey bee chemosensing of V. destructor

Can We Disrupt the Sensing of Honey Bees by the Bee Parasite Varroa destructor?

Background The ectoparasitic mite, Varroa destructor, is considered to be one of the most significant threats to apiculture around the world. Chemical cues are known to play a significant role in the host-finding behavior of Varroa. The mites distinguish between bees from different task groups, and prefer nurses over foragers. We examined the possibility of disrupting the Varroa – honey bee interaction by targeting the mite\u27s olfactory system. In particular, we examined the effect of volatile compounds, ethers of cis 5-(2′-hydroxyethyl) cyclopent-2-en-1-ol or of dihydroquinone, resorcinol or catechol. We tested the effect of these compounds on the Varroa chemosensory organ by electrophysiology and on behavior in a choice bioassay. The electrophysiological studies were conducted on the isolated foreleg. In the behavioral bioassay, the mite\u27s preference between a nurse and a forager bee was evaluated. Principal findings We found that in the presence of some compounds, the response of the Varroa chemosensory organ to honey bee headspace volatiles significantly decreased. This effect was dose dependent and, for some of the compounds, long lasting (>1 min). Furthermore, disruption of the Varroa volatile detection was accompanied by a reversal of the mite\u27s preference from a nurse to a forager bee. Long-term inhibition of the electrophysiological responses of mites to the tested compounds was a good predictor for an alteration in the mite\u27s host preference. Conclusions These data indicate the potential of the selected compounds to disrupt the Varroa - honey bee associations, thus opening new avenues for Varroa control