Kajian Morfologi, Kepatogenan Dan Molekular Pencilan Fusarium Daripada Orkid

Sebanyak 32 pencilan Fusarium telah dipencilkan dari orkid Dendrobium (29 pencilan) dan Oncidium (3 pencilan) yang menunjukkan gejala reput akar dan reput batang. A total of 32 Fusarium spp were isolated from Dendrobium (29 isolates) and Oncidium (3 isolates) showing symptoms of root and stem rot. Another eight isolates were obtained from Fusarium stock cultures of USM

Kajian Morfologi, Kepatogenan Dan Molekular Pencilan Fusarium Daripada Orkid [SB741.F9 N974 2007 f rb].

Sebanyak 32 pencilan Fusarium telah dipencilkan dari orkid Dendrobium (29 pencilan) dan Oncidium (3 pencilan) yang menunjukkan gejala reput akar dan reput batang. Lapan pencilan Fusarium yang dipencilkan dari orkid juga turut diperolehi dari Himpunan Kultur Fusarium USM. A total of 32 Fusarium spp were isolated from Dendrobium (29 isolates) and Oncidium (3 isolates) showing symptoms of root and stem rot. Another eight isolates were obtained from Fusarium stock cultures of US

Morus alba L. : creating miles of smiles / Nurhayati Mohamad Zain … [et al.]

The aims of the study are to determine the antimicrobial and antibiofilm activities of M. alba leaves extract against Streptococcus mutans (S.mutans). Method: The antimicrobial activity was evaluated using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The antibiofilm potential of extract was evaluated using biofilm inhibition assay and biofilm eradication assay. Oradex mouthwash with 0.12% CHX was used as a positive control. All experiments were performed in triplicate and repeated four times independently. Data were analysed using SPSS software version 23. A Kruskal-Wallis test followed by post-hoc Mann-Whitney U test was applied and level of significance was set at P < 0.001. Results: Morus alba (M.alba) leaves extract showed antimicrobial activity against S.mutans with MIC and MBC, 25mg/ml and 50mg/ml respectively. The adherence of bacteria on extract treated surface (0.5x MIC) was significantly reduced with adherence inhibition percentage of 72.5% compared to positive control CHX 0.12% (63%). At 0.5x MIC concentration, the extract also disrupted preformed biofilms with eradication percentage of 52.87%. Conclusion: The results suggest that M. alba leaves extract represents an untapped source of local plant with antibiofilm activity against S.mutans that could be a resource in the development of therapeutic natural products in managing dental caries

The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus

The antimicrobial and antibiofilm activities of sweet basil essential oil (SBEO) against oral microorganisms, Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus) were determined in this study. The antimicrobial activities were evaluated using the disk diffusion method (DDM), where Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were tested against both bacteria. Different formulations of SBEO (microemulsion, emulsion, water) were tested for biofilm dispersion assay on 24 h of preformed biofilm. Commercial chlorhexidine (CHX) 0.12% w/v was used as a positive control. The scanning electron microscope (SEM) was used to observe the changes on the treated surface. The data were analyzed using SPSS Version 27.0. A Kruskal-Wallis test followed by a Post-hoc Mann-Whitney U test was applied, and the level of significance was set at p<0.05. All formulations of SBEO showed antimicrobial activities against the tested microorganisms. Exposure to 2.5% (v/v) microemulsion for two min exhibited 42.56% and 32.10% (p<0.001) of biofilm dispersion for S. mutans and S. aureus, respectively. The SEM micrographs revealed the number of microorganisms on the treated group surface reduced compared to the negative controls. SBEO exerts an antimicrobial and antibiofilm effect on S. mutans and S. aureus. This finding suggests that the SBEO microemulsion has the potential to control planktonic S. mutans and S. aureus and their biofilm formation in the oral environment

The antimicrobial and antibiofilm potential of sweet basil essential oil on Streptococcus mutans and Staphylococcus aureus

The antimicrobial and antibiofilm activities of sweet basil essential oil (SBEO) against oral microorganisms, Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus) were determined in this study. The antimicrobial activities were evaluated using the disk diffusion method (DDM), where Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were tested against both bacteria. Different formulations of SBEO (microemulsion, emulsion, water) were tested for biofilm dispersion assay on 24 h of preformed biofilm. Commercial chlorhexidine (CHX) 0.12% w/v was used as a positive control. The scanning electron microscope (SEM) was used to observe the changes on the treated surface. The data were analyzed using SPSS Version 27.0. A Kruskal-Wallis test followed by a Post-hoc Mann-Whitney U test was applied, and the level of significance was set at p<0.05. All formulations of SBEO showed antimicrobial activities against the tested microorganisms. Exposure to 2.5% (v/v) microemulsion for two min exhibited 42.56% and 32.10% (p<0.001) of biofilm dispersion for S. mutans and S. aureus, respectively. The SEM micrographs revealed the number of microorganisms on the treated group surface reduced compared to the negative controls. SBEO exerts an antimicrobial and antibiofilm effect on S. mutans and S. aureus. This finding suggests that the SBEO microemulsion has the potential to control planktonic S. mutans and S. aureus and their biofilm formation in the oral environment